5euc - Revision history

OCA at 08:24, 12 July 2023

OCA — Wed, 12 Jul 2023 08:24:40 GMT

←Older revision		Revision as of 08:24, 12 July 2023
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	<StructureSection load='5euc' size='340' side='right'caption='[[5euc]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5euc' size='340' side='right'caption='[[5euc]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5euc]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Trycc Trycc~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EUC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5EUC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5euc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi_strain_CL_Brener Trypanosoma cruzi strain CL Brener]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EUC FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Tc00.1047053509693.70 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=353153 TRYCC])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5euc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5euc OCA], [~~http~~://pdbe.org/5euc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5euc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5euc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5euc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5euc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5euc OCA], [https://pdbe.org/5euc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5euc RCSB], [https://www.ebi.ac.uk/pdbsum/5euc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5euc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4DRC4_TRYCC Q4DRC4_TRYCC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Trycc~~]]	+	[[Category: Trypanosoma cruzi strain CL Brener]]
-	[[Category: Cousido-Siah, A]]	+	[[Category: Cousido-Siah A]]
-	[[Category: Delfino~~, J M~~]]	+	[[Category: Delfino JM]]
-	[[Category: Mitschler, A]]	+	[[Category: Mitschler A]]
-	[[Category: Podjarny, A]]	+	[[Category: Podjarny A]]
-	[[Category: Santos, J]]	+	[[Category: Santos J]]
-	[[Category: Valsecchi~~, W M]]~~	+	[[Category: Valsecchi WM]]
-	~~[[Category: Bisphosphonate]]~~	+
-	~~[[Category: Disorder c-terminal region]]~~	+
-	~~[[Category: Enzymatic activity modulation]]~~	+
-	~~[[Category: Hprt]]~~	+
-	~~[[Category: Phosphoribosyltransferase]]~~	+
-	~~[[Category: Proteolysis]]~~	+
-	~~[[Category: Quaternary structure]]~~	+
-	~~[[Category: Reversible oligomerization]]~~	+
-	~~[[Category: Stability]]~~	+
-	~~[[Category: T. cruzi]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:45, 29 April 2020

OCA — Wed, 29 Apr 2020 07:45:03 GMT

←Older revision		Revision as of 07:45, 29 April 2020
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	==The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase==		==The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase==
-	<StructureSection load='5euc' size='340' side='right' caption='[[5euc]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='5euc' size='340' side='right'caption='[[5euc]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5euc]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EUC OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EUC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5euc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycc Trycc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EUC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EUC FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5euc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5euc OCA], [http://pdbe.org/5euc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5euc RCSB], [http://www.ebi.ac.uk/pdbsum/5euc PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tc00.1047053509693.70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=353153 TRYCC])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5euc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5euc OCA], [http://pdbe.org/5euc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5euc RCSB], [http://www.ebi.ac.uk/pdbsum/5euc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5euc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Trycc]]
	[[Category: Cousido-Siah, A]]		[[Category: Cousido-Siah, A]]
	[[Category: Delfino, J M]]		[[Category: Delfino, J M]]

OCA at 20:32, 11 May 2016

OCA — Wed, 11 May 2016 20:32:51 GMT

←Older revision		Revision as of 20:32, 11 May 2016
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-	'''Unreleased structure'''

-	The ~~entry~~ 5euc is ~~ON HOLD until Paper~~ Publication	+	==The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase==
		+	<StructureSection load='5euc' size='340' side='right' caption='[[5euc]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5euc]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EUC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EUC FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5euc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5euc OCA], [http://pdbe.org/5euc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5euc RCSB], [http://www.ebi.ac.uk/pdbsum/5euc PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hypoxanthine phosphoribosyl transferase from Trypanosoma cruzi (TcHPRT) is a critical enzyme for the survival of the parasite. This work demonstrates that the full-length form in solution adopts a stable and enzymatically active tetrameric form, exhibiting large inter-subunit surfaces. Although this protein irreversibly aggregates during unfolding, oligomerization is reversible and can be modulated by low concentrations of urea. When the C-terminal region, which is predicted as a disordered stretch, is excised by proteolysis, TcHPRT adopts a dimeric state, suggesting that the C-terminal region acts as a main guide for the quaternary arrangement. These results are in agreement with X-ray crystallographic data presented in this work. On the other hand, the C-terminal region exhibits a modulatory role on the enzyme, as attested by the enhanced activity observed for the dimeric form. Bisphosphonates act as substrate-mimetics, uncovering long-range communications among the active sites. All in all, this work contributes to establish new ways applicable to the design of novel inhibitors that could eventually result in new drugs against parasitic diseases.

-	~~Authors: Valsecchi, W.M~~., Cousido-Siah, A., Mitschler, A., Podjarny, A~~., Delfino~~, J.M., ~~Santos, J~~.	+	The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase.,Valsecchi WM, Cousido-Siah A, Defelipe LA, Mitschler A, Podjarny A, Santos J, Delfino JM Biochim Biophys Acta. 2016 Jun;1864(6):655-66. doi: 10.1016/j.bbapap.2016.03.005., Epub 2016 Mar 10. PMID:26969784<ref>PMID:26969784</ref>

-	~~Description: The role~~ of the ~~C-terminal region on the oligomeric state and enzymatic activity~~ of ~~Trypanosoma cruzi hypoxanthine phosphoribosyl transferase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Podjarny~~, A]]	+	<div class="pdbe-citations 5euc" style="background-color:#fffaf0;"></div>
-	[[Category: Delfino, J~~.M]]~~	+	== References ==
-	~~[[Category: Valsecchi, W.~~M]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cousido-Siah, A]]
		+	[[Category: Delfino, J M]]
	[[Category: Mitschler, A]]		[[Category: Mitschler, A]]
		+	[[Category: Podjarny, A]]
	[[Category: Santos, J]]		[[Category: Santos, J]]
-	[[Category: ~~Cousido-Siah~~, A]]	+	[[Category: Valsecchi, W M]]
		+	[[Category: Bisphosphonate]]
		+	[[Category: Disorder c-terminal region]]
		+	[[Category: Enzymatic activity modulation]]
		+	[[Category: Hprt]]
		+	[[Category: Phosphoribosyltransferase]]
		+	[[Category: Proteolysis]]
		+	[[Category: Quaternary structure]]
		+	[[Category: Reversible oligomerization]]
		+	[[Category: Stability]]
		+	[[Category: T. cruzi]]
		+	[[Category: Transferase]]

OCA at 12:15, 16 December 2015

OCA — Wed, 16 Dec 2015 12:15:58 GMT

←Older revision		Revision as of 12:15, 16 December 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5euc is ON HOLD	+	The entry 5euc is ON HOLD until Paper Publication

	Authors: Valsecchi, W.M., Cousido-Siah, A., Mitschler, A., Podjarny, A., Delfino, J.M., Santos, J.		Authors: Valsecchi, W.M., Cousido-Siah, A., Mitschler, A., Podjarny, A., Delfino, J.M., Santos, J.

OCA: Protected "5euc" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 03:22:47 GMT

Protected "5euc" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:22, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5euc is ON HOLD Authors: Valsecchi, W.M., Cousido-Siah, A., Mitschler, A., Podjarny, A., Delfino, J.M., Santos, J. Description: The role of the C-t...

OCA — Tue, 01 Dec 2015 03:22:46 GMT

New page: '''Unreleased structure''' The entry 5euc is ON HOLD Authors: Valsecchi, W.M., Cousido-Siah, A., Mitschler, A., Podjarny, A., Delfino, J.M., Santos, J. Description: The role of the C-t...

New page

'''Unreleased structure'''

The entry 5euc is ON HOLD

Authors: Valsecchi, W.M., Cousido-Siah, A., Mitschler, A., Podjarny, A., Delfino, J.M., Santos, J.

Description: The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase
[[Category: Unreleased Structures]]
[[Category: Podjarny, A]]
[[Category: Delfino, J.M]]
[[Category: Valsecchi, W.M]]
[[Category: Mitschler, A]]
[[Category: Santos, J]]
[[Category: Cousido-Siah, A]]