5f0e - Revision history

OCA at 08:38, 12 July 2023

OCA — Wed, 12 Jul 2023 08:38:15 GMT

←Older revision		Revision as of 08:38, 12 July 2023
Line 3:		Line 3:
	<StructureSection load='5f0e' size='340' side='right'caption='[[5f0e]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='5f0e' size='340' side='right'caption='[[5f0e]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5f0e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F0E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5F0E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5f0e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F0E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.74Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.84 3.2.1.84] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f0e OCA], [~~http~~://pdbe.org/5f0e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5f0e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5f0e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5f0e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f0e OCA], [https://pdbe.org/5f0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f0e RCSB], [https://www.ebi.ac.uk/pdbsum/5f0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f0e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/GANAB_MOUSE GANAB_MOUSE]] Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. [[http://www.uniprot.org/uniprot/GLU2B_MOUSE GLU2B_MOUSE]] Regulatory subunit of glucosidase II.<ref>PMID:9148925</ref>	+	[https://www.uniprot.org/uniprot/GLU2B_MOUSE GLU2B_MOUSE] Regulatory subunit of glucosidase II.<ref>PMID:9148925</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hydrolase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Alonzi, D S~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Caputo, A T~~]]	+	[[Category: Alonzi DS]]
-	[[Category: ~~Kiappes, J L~~]]	+	[[Category: Caputo AT]]
-	[[Category: ~~Roversi, P~~]]	+	[[Category: Kiappes JL]]
-	[[Category: ~~Zitzmann, N~~]]	+	[[Category: Roversi P]]
-	[[Category: ~~Enzyme glycosyl hydrolase gh31 quality control exoglycosidase~~]]	+	[[Category: Zitzmann N]]

OCA at 07:53, 29 April 2020

OCA — Wed, 29 Apr 2020 07:53:42 GMT

←Older revision		Revision as of 07:53, 29 April 2020
Line 1:		Line 1:

	==Murine endoplasmic reticulum alpha-glucosidase II==		==Murine endoplasmic reticulum alpha-glucosidase II==
-	<StructureSection load='5f0e' size='340' side='right' caption='[[5f0e]], [[Resolution\|resolution]] 1.74Å' scene=''>	+	<StructureSection load='5f0e' size='340' side='right'caption='[[5f0e]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5f0e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F0E OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5F0E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5f0e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F0E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5F0E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.84 3.2.1.84] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.84 3.2.1.84] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f0e OCA], [http://pdbe.org/5f0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f0e RCSB], [http://www.ebi.ac.uk/pdbsum/5f0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f0e ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f0e OCA], [http://pdbe.org/5f0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f0e RCSB], [http://www.ebi.ac.uk/pdbsum/5f0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f0e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5f0e" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5f0e" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Alpha-glucosidase 3D structures\|Alpha-glucosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
		+	[[Category: Large Structures]]
	[[Category: Alonzi, D S]]		[[Category: Alonzi, D S]]
	[[Category: Caputo, A T]]		[[Category: Caputo, A T]]

OCA at 01:14, 16 November 2017

OCA — Thu, 16 Nov 2017 01:14:14 GMT

←Older revision		Revision as of 01:14, 16 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5f0e" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5f0e" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Alpha-glucosidase\|Alpha-glucosidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:54, 17 August 2017

OCA — Thu, 17 Aug 2017 05:54:29 GMT

←Older revision		Revision as of 05:54, 17 August 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5f0e" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5f0e" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Alpha-glucosidase\|Alpha-glucosidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:32, 3 August 2016

OCA — Wed, 03 Aug 2016 20:32:14 GMT

←Older revision		Revision as of 20:32, 3 August 2016
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GANAB_MOUSE GANAB_MOUSE]] Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. [[http://www.uniprot.org/uniprot/GLU2B_MOUSE GLU2B_MOUSE]] Regulatory subunit of glucosidase II.<ref>PMID:9148925</ref>		[[http://www.uniprot.org/uniprot/GANAB_MOUSE GANAB_MOUSE]] Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. [[http://www.uniprot.org/uniprot/GLU2B_MOUSE GLU2B_MOUSE]] Regulatory subunit of glucosidase II.<ref>PMID:9148925</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The biosynthesis of enveloped viruses depends heavily on the host cell endoplasmic reticulum (ER) glycoprotein quality control (QC) machinery. This dependency exceeds the dependency of host glycoproteins, offering a window for the targeting of ERQC for the development of broad-spectrum antivirals. We determined small-angle X-ray scattering (SAXS) and crystal structures of the main ERQC enzyme, ER alpha-glucosidase II (alpha-GluII; from mouse), alone and in complex with key ligands of its catalytic cycle and antiviral iminosugars, including two that are in clinical trials for the treatment of dengue fever. The SAXS data capture the enzyme's quaternary structure and suggest a conformational rearrangement is needed for the simultaneous binding of a monoglucosylated glycan to both subunits. The X-ray structures with key catalytic cycle intermediates highlight that an insertion between the +1 and +2 subsites contributes to the enzyme's activity and substrate specificity, and reveal that the presence of d-mannose at the +1 subsite renders the acid catalyst less efficient during the cleavage of the monoglucosylated substrate. The complexes with iminosugar antivirals suggest that inhibitors targeting a conserved ring of aromatic residues between the alpha-GluII +1 and +2 subsites would have increased potency and selectivity, thus providing a template for further rational drug design.
		+
		+	Structures of mammalian ER alpha-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.,Caputo AT, Alonzi DS, Marti L, Reca IB, Kiappes JL, Struwe WB, Cross A, Basu S, Lowe ED, Darlot B, Santino A, Roversi P, Zitzmann N Proc Natl Acad Sci U S A. 2016 Jul 26. pii: 201604463. PMID:27462106<ref>PMID:27462106</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5f0e" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:38, 27 July 2016

OCA — Wed, 27 Jul 2016 15:38:47 GMT

←Older revision		Revision as of 15:38, 27 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5f0e is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Murine endoplasmic reticulum alpha-glucosidase II==
-		+	<StructureSection load='5f0e' size='340' side='right' caption='[[5f0e]], [[Resolution\|resolution]] 1.74Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5f0e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F0E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F0E FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.84 3.2.1.84] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f0e OCA], [http://pdbe.org/5f0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f0e RCSB], [http://www.ebi.ac.uk/pdbsum/5f0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f0e ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GANAB_MOUSE GANAB_MOUSE]] Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. [[http://www.uniprot.org/uniprot/GLU2B_MOUSE GLU2B_MOUSE]] Regulatory subunit of glucosidase II.<ref>PMID:9148925</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hydrolase]]
		+	[[Category: Alonzi, D S]]
		+	[[Category: Caputo, A T]]
		+	[[Category: Kiappes, J L]]
		+	[[Category: Roversi, P]]
		+	[[Category: Zitzmann, N]]
		+	[[Category: Enzyme glycosyl hydrolase gh31 quality control exoglycosidase]]

OCA at 10:25, 20 June 2016

OCA — Mon, 20 Jun 2016 10:25:26 GMT

←Older revision		Revision as of 10:25, 20 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5f0e is ON HOLD	+	The entry 5f0e is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 12:42, 15 May 2016

OCA — Sun, 15 May 2016 12:42:22 GMT

←Older revision		Revision as of 12:42, 15 May 2016
Line 3:		Line 3:
	The entry 5f0e is ON HOLD		The entry 5f0e is ON HOLD

-	Authors: ~~Caputo, A.T., Roversi, P., Alonzi, D.S., Kiappes, J.L., Zitzmann, N.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Zitzmann, N]]
-	[[Category: Alonzi, D.S]]
-	[[Category: Kiappes, J.L]]
-	[[Category: Caputo, A.T]]
-	[[Category: Roversi, P]]

OCA at 22:37, 10 May 2016

OCA — Tue, 10 May 2016 22:37:57 GMT

←Older revision		Revision as of 22:37, 10 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5f0e is ON HOLD ~~until Paper Publication~~	+	The entry 5f0e is ON HOLD

	Authors: Caputo, A.T., Roversi, P., Alonzi, D.S., Kiappes, J.L., Zitzmann, N.		Authors: Caputo, A.T., Roversi, P., Alonzi, D.S., Kiappes, J.L., Zitzmann, N.

OCA at 17:22, 30 December 2015

OCA — Wed, 30 Dec 2015 17:22:42 GMT

←Older revision		Revision as of 17:22, 30 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5f0e is ON HOLD	+	The entry 5f0e is ON HOLD until Paper Publication

	Authors: Caputo, A.T., Roversi, P., Alonzi, D.S., Kiappes, J.L., Zitzmann, N.		Authors: Caputo, A.T., Roversi, P., Alonzi, D.S., Kiappes, J.L., Zitzmann, N.