5f1k - Revision history

OCA at 08:40, 12 July 2023

OCA — Wed, 12 Jul 2023 08:40:17 GMT

←Older revision		Revision as of 08:40, 12 July 2023
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	<StructureSection load='5f1k' size='340' side='right'caption='[[5f1k]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5f1k' size='340' side='right'caption='[[5f1k]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5f1k]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus glama~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F1K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5F1K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5f1k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F1K FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CD38 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=9606 HUMAN])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ADP~~-~~ribosyl_cyclase/cyclic_ADP-ribose_hydrolase ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase]~~, ~~with EC number~~ [~~http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.~~2.~~2.6~~ 3~~.2.2.6] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f1k OCA], [https://pdbe.org/5f1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f1k RCSB], [https://www.ebi.ac.uk/pdbsum/5f1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f1k ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f1k OCA], [~~http~~://pdbe.org/5f1k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5f1k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5f1k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5f1k ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CD38_HUMAN CD38_HUMAN]] Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.	+	[https://www.uniprot.org/uniprot/CD38_HUMAN CD38_HUMAN] Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Camelus~~ glama]]	+	[[Category: Lama glama]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hao, Q]]	+	[[Category: Hao Q]]
-	[[Category: Zhang, H]]	+	[[Category: Zhang H]]
-	~~[[Category: Adp-ribosyl cyclase]]~~	+
-	~~[[Category: Calcium signaling]]~~	+
-	~~[[Category: Cd38]]~~	+
-	~~[[Category: Cyclic adp-ribose]]~~	+
-	~~[[Category: Hydrolase-immune system complex]]~~	+
-	~~[[Category: Mu1053]]~~	+
-	~~[[Category: Nanobody]]~~	+
-	~~[[Category: X-crystallography~~]]	+

OCA at 07:55, 29 April 2020

OCA — Wed, 29 Apr 2020 07:55:21 GMT

←Older revision		Revision as of 07:55, 29 April 2020
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	==human CD38 in complex with nanobody MU1053==		==human CD38 in complex with nanobody MU1053==
-	<StructureSection load='5f1k' size='340' side='right' caption='[[5f1k]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5f1k' size='340' side='right'caption='[[5f1k]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5f1k]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F1K OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5F1K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5f1k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F1K OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5F1K FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ADP-ribosyl_cyclase/cyclic_ADP-ribose_hydrolase ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.6 3.2.2.6] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CD38 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f1k OCA], [http://pdbe.org/5f1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f1k RCSB], [http://www.ebi.ac.uk/pdbsum/5f1k PDBsum]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ADP-ribosyl_cyclase/cyclic_ADP-ribose_hydrolase ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.6 3.2.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f1k OCA], [http://pdbe.org/5f1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f1k RCSB], [http://www.ebi.ac.uk/pdbsum/5f1k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f1k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase]]		[[Category: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase]]
		+	[[Category: Camelus glama]]
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hao, Q]]		[[Category: Hao, Q]]
	[[Category: Zhang, H]]		[[Category: Zhang, H]]

OCA at 15:15, 20 June 2016

OCA — Mon, 20 Jun 2016 15:15:36 GMT

←Older revision		Revision as of 15:15, 20 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5f1k is ~~ON HOLD until Paper~~ Publication	+	==human CD38 in complex with nanobody MU1053==
		+	<StructureSection load='5f1k' size='340' side='right' caption='[[5f1k]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5f1k]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F1K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F1K FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ADP-ribosyl_cyclase/cyclic_ADP-ribose_hydrolase ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.6 3.2.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f1k OCA], [http://pdbe.org/5f1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f1k RCSB], [http://www.ebi.ac.uk/pdbsum/5f1k PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CD38_HUMAN CD38_HUMAN]] Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CD38, as a cell surface antigen is highly expressed in several hematologic malignancies including multiple myeloma (MM) and has been proven to be a good target for immunotherapy of the disease. CD38 is also a signaling enzyme responsible for the metabolism of two novel calcium messenger molecules. To be able to target this multifunctional protein, we generated a series of nanobodies against CD38 with high affinities. Crystal structures of the complexes of CD38 with the nanobodies were solved, identifying three separate epitopes on the carboxyl domain. Chromobodies, engineered by tagging the nanobody with fluorescence proteins, provide fast, simple and versatile tools for quantifying CD38 expression. Results confirmed that CD38 was highly expressed in malignant MM cells compared with normal white blood cells. The immunotoxin constructed by splicing the nanobody with a bacterial toxin, PE38 shows highly selective cytotoxicity against patient-derived MM cells as well as the cell lines, with half maximal effective concentration reaching as low as 10(-11) molar. The effectiveness of the immunotoxin can be further increased by stimulating CD38 expression using retinoid acid. These results set the stage for the development of clinical therapeutics as well as diagnostic screening for myeloma.

-	~~Authors:~~ Zhang, H., Hao, Q.	+	Immuno-targeting the multifunctional CD38 using nanobody.,Li T, Qi S, Unger M, Hou YN, Deng QW, Liu J, Lam CM, Wang XW, Xin D, Zhang P, Koch-Nolte F, Hao Q, Zhang H, Lee HC, Zhao YJ Sci Rep. 2016 Jun 2;6:27055. doi: 10.1038/srep27055. PMID:27251573<ref>PMID:27251573</ref>

-	~~Description~~: ~~human CD38 in complex with nanobody MU1053~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5f1k" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase]]
	[[Category: Hao, Q]]		[[Category: Hao, Q]]
	[[Category: Zhang, H]]		[[Category: Zhang, H]]
		+	[[Category: Adp-ribosyl cyclase]]
		+	[[Category: Calcium signaling]]
		+	[[Category: Cd38]]
		+	[[Category: Cyclic adp-ribose]]
		+	[[Category: Hydrolase-immune system complex]]
		+	[[Category: Mu1053]]
		+	[[Category: Nanobody]]
		+	[[Category: X-crystallography]]

OCA: Protected "5f1k" [edit=sysop:move=sysop]

OCA — Wed, 09 Dec 2015 12:29:07 GMT

Protected "5f1k" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:29, 9 December 2015

OCA: New page: '''Unreleased structure''' The entry 5f1k is ON HOLD until Paper Publication Authors: Zhang, H., Hao, Q. Description: human CD38 in complex with nanobody MU1053 [[Category: Unreleased ...

OCA — Wed, 09 Dec 2015 12:29:05 GMT

New page: '''Unreleased structure''' The entry 5f1k is ON HOLD until Paper Publication Authors: Zhang, H., Hao, Q. Description: human CD38 in complex with nanobody MU1053 [[Category: Unreleased ...

New page

'''Unreleased structure'''

The entry 5f1k is ON HOLD until Paper Publication

Authors: Zhang, H., Hao, Q.

Description: human CD38 in complex with nanobody MU1053
[[Category: Unreleased Structures]]
[[Category: Hao, Q]]
[[Category: Zhang, H]]