5f51 - Revision history

OCA at 08:46, 12 July 2023

OCA — Wed, 12 Jul 2023 08:46:47 GMT

←Older revision		Revision as of 08:46, 12 July 2023
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	<StructureSection load='5f51' size='340' side='right'caption='[[5f51]], [[Resolution\|resolution]] 2.53Å' scene=''>		<StructureSection load='5f51' size='340' side='right'caption='[[5f51]], [[Resolution\|resolution]] 2.53Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5f51]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Brua2 Brua2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F51 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5F51 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5f51]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus_2308 Brucella abortus 2308]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F51 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.53Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[5f4b\|5f4b~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>BAB1_1070 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~359391 BRUA2])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f51 OCA], [https://pdbe.org/5f51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f51 RCSB], [https://www.ebi.ac.uk/pdbsum/5f51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f51 ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/NAD(P)H_dehydrogenase_(quinone) NAD(P)H dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.2 1.6.5.2]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5f51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f51 OCA], [~~http~~://pdbe.org/5f51 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5f51 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5f51 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5f51 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NQOR_BRUA2 NQOR_BRUA2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Brua2~~]]	+	[[Category: Brucella abortus 2308]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Crosson, S]]	+	[[Category: Crosson S]]
-	[[Category: Czyz, D]]	+	[[Category: Czyz D]]
-	[[Category: Herrou, J]]	+	[[Category: Herrou J]]
-	[[Category: Kim~~, H S~~]]	+	[[Category: Kim HS]]
-	[[Category: Kim, Y]]	+	[[Category: Kim Y]]
-	[[Category: Willett~~, J W]]~~	+	[[Category: Willett JW]]
-	~~[[Category: Brucella abortus]]~~	+
-	~~[[Category: Nadh:quinone]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Wrba]]~~	+
-	~~[[Category: Wrpa~~]]	+

OCA at 16:09, 11 December 2019

OCA — Wed, 11 Dec 2019 16:09:01 GMT

←Older revision		Revision as of 16:09, 11 December 2019
Line 1:		Line 1:

	==Structure of B. abortus WrbA-related protein A (apo)==		==Structure of B. abortus WrbA-related protein A (apo)==
-	<StructureSection load='5f51' size='340' side='right' caption='[[5f51]], [[Resolution\|resolution]] 2.53Å' scene=''>	+	<StructureSection load='5f51' size='340' side='right'caption='[[5f51]], [[Resolution\|resolution]] 2.53Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5f51]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brua2 Brua2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F51 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5f51]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brua2 Brua2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F51 FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Brua2]]		[[Category: Brua2]]
		+	[[Category: Large Structures]]
	[[Category: Crosson, S]]		[[Category: Crosson, S]]
	[[Category: Czyz, D]]		[[Category: Czyz, D]]

OCA at 12:41, 6 November 2017

OCA — Mon, 06 Nov 2017 12:41:07 GMT

←Older revision		Revision as of 12:41, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5f51' size='340' side='right' caption='[[5f51]], [[Resolution\|resolution]] 2.53Å' scene=''>		<StructureSection load='5f51' size='340' side='right' caption='[[5f51]], [[Resolution\|resolution]] 2.53Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5f51]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F51 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5f51]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brua2 Brua2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F51 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5f4b\|5f4b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5f4b\|5f4b]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAB1_1070 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=359391 BRUA2])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(P)H_dehydrogenase_(quinone) NAD(P)H dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.2 1.6.5.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(P)H_dehydrogenase_(quinone) NAD(P)H dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.2 1.6.5.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f51 OCA], [http://pdbe.org/5f51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f51 RCSB], [http://www.ebi.ac.uk/pdbsum/5f51 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f51 OCA], [http://pdbe.org/5f51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f51 RCSB], [http://www.ebi.ac.uk/pdbsum/5f51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f51 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Brua2]]
	[[Category: Crosson, S]]		[[Category: Crosson, S]]
	[[Category: Czyz, D]]		[[Category: Czyz, D]]

OCA at 03:38, 10 March 2016

OCA — Thu, 10 Mar 2016 03:38:48 GMT

←Older revision		Revision as of 03:38, 10 March 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5f51 is ~~ON HOLD~~	+	==Structure of B. abortus WrbA-related protein A (apo)==
		+	<StructureSection load='5f51' size='340' side='right' caption='[[5f51]], [[Resolution\|resolution]] 2.53Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5f51]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F51 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5f4b\|5f4b]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(P)H_dehydrogenase_(quinone) NAD(P)H dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.2 1.6.5.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f51 OCA], [http://pdbe.org/5f51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f51 RCSB], [http://www.ebi.ac.uk/pdbsum/5f51 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The general stress response (GSR) system of the intracellular pathogen Brucella abortus controls transcription of approximately 100 genes in response to a range of stress cues. The core genetic regulatory components of the GSR are required for B. abortus survival under non-optimal growth conditions in vitro, and for maintenance of chronic infection in an in vivo mouse model. The functions of the majority of the genes in the GSR transcriptional regulon remain undefined. bab1_1070 is among the most highly regulated genes in this regulon: its transcription is activated 20-30 fold by the GSR system under oxidative conditions in vitro. We have solved crystal structures of Bab1_1070 and demonstrate that it forms a homotetrameric complex that resembles WrbA-type NADH:quinone oxidoreductases, which are members of the flavodoxin protein family. However, B. abortus WrbA-related protein (WrpA) does not bind flavin cofactors with high affinity and does not function as an NADH:quinone oxidoreductase in vitro. Soaking crystals with flavin mononucleotide (FMN) revealed a likely low-affinity binding site adjacent to the canonical WrbA flavin binding site. Deletion of wrpA (DeltawrpA) does not compromise cell survival under acute oxidative stress in vitro or attenuate infection in cell-based or mouse models. However, a DeltawrpA strain does elicit increased splenomegaly in a mouse model suggesting that WrpA modulates B. abortus interaction with its mammalian host. Despite high structural homology with canonical WrbA proteins, we propose that B. abortus WrpA represents a functionally-distinct member of the diverse flavodoxin family. IMPORTANCE: Brucella abortus is an etiological agent of brucellosis, which is among the most common zoonotic diseases worldwide. The general stress response (GSR) regulatory system of B. abortus controls transcription of approximately 100 genes and is required for maintenance of chronic infection in a murine model; the majority of GSR-regulated genes remain uncharacterized. We present in vitro and in vivo functional and structural analyses of WrpA, whose expression is strongly induced by GSR under oxidative conditions. Though WrpA is structurally related to NADH:quinone oxidoreductases, it does not bind redox cofactors in solution, nor does it exhibit oxidoreductase activity in vitro. However, WrpA does affect spleen inflammation in a murine infection model. Our data provide evidence that WrpA forms a new functional class of WrbA/flavodoxin-family proteins.

-	~~Authors: Herrou~~, J., Czyz~~, D.~~, Willett~~, J.W.~~, Kim, ~~H.S.~~, Kim, Y., Crosson, S.	+	WrpA is an atypical flavodoxin-family protein under regulatory control of the Brucella abortus general stress response system.,Herrou J, Czyz DM, Willett JW, Kim HS, Chhor G, Babnigg G, Kim Y, Crosson S J Bacteriol. 2016 Feb 8. pii: JB.00982-15. PMID:26858101<ref>PMID:26858101</ref>

-	~~Description: Structure~~ of B. ~~abortus WrbA-related protein A (apo)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Willett, J.W]]~~	+	<div class="pdbe-citations 5f51" style="background-color:#fffaf0;"></div>
-	~~[[Category: Herrou, J]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Crosson, S]]		[[Category: Crosson, S]]
-	[[Category: Kim, H.S]]
-	[[Category: Kim, Y]]
	[[Category: Czyz, D]]		[[Category: Czyz, D]]
		+	[[Category: Herrou, J]]
		+	[[Category: Kim, H S]]
		+	[[Category: Kim, Y]]
		+	[[Category: Willett, J W]]
		+	[[Category: Brucella abortus]]
		+	[[Category: Nadh:quinone]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Wrba]]
		+	[[Category: Wrpa]]

OCA: Protected "5f51" [edit=sysop:move=sysop]

OCA — Wed, 09 Dec 2015 12:38:45 GMT

Protected "5f51" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:38, 9 December 2015

OCA: New page: '''Unreleased structure''' The entry 5f51 is ON HOLD Authors: Herrou, J., Czyz, D., Willett, J.W., Kim, H.S., Kim, Y., Crosson, S. Description: Structure of B. abortus WrbA-related pro...

OCA — Wed, 09 Dec 2015 12:38:44 GMT

New page: '''Unreleased structure''' The entry 5f51 is ON HOLD Authors: Herrou, J., Czyz, D., Willett, J.W., Kim, H.S., Kim, Y., Crosson, S. Description: Structure of B. abortus WrbA-related pro...

New page

'''Unreleased structure'''

The entry 5f51 is ON HOLD

Authors: Herrou, J., Czyz, D., Willett, J.W., Kim, H.S., Kim, Y., Crosson, S.

Description: Structure of B. abortus WrbA-related protein A (apo)
[[Category: Unreleased Structures]]
[[Category: Willett, J.W]]
[[Category: Herrou, J]]
[[Category: Crosson, S]]
[[Category: Kim, H.S]]
[[Category: Kim, Y]]
[[Category: Czyz, D]]