5fbs - Revision history

OCA at 06:45, 19 July 2023

OCA — Wed, 19 Jul 2023 06:45:40 GMT

←Older revision		Revision as of 06:45, 19 July 2023
Line 3:		Line 3:
	<StructureSection load='5fbs' size='340' side='right'caption='[[5fbs]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='5fbs' size='340' side='right'caption='[[5fbs]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fbs]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lismf Lismf~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FBS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fbs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_serotype_4b_str._F2365 Listeria monocytogenes serotype 4b str. F2365]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FBS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.59Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5fbt\|5fbt]]~~, ~~[[5fbu\|5fbu]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbs OCA], [~~http~~://pdbe.org/5fbs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fbs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fbs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fbs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbs OCA], [https://pdbe.org/5fbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fbs RCSB], [https://www.ebi.ac.uk/pdbsum/5fbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fbs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lismf~~]]	+	[[Category: Listeria monocytogenes serotype 4b str. F2365]]
-	[[Category: Anderson~~, W F]]~~	+	[[Category: Anderson WF]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Savchenko A]]
-	[[Category: Savchenko, A]]	+	[[Category: Skarina T]]
-	[[Category: Skarina, T]]	+	[[Category: Stogios PJ]]
-	[[Category: Stogios~~, P J~~]]	+	[[Category: Wawrzak Z]]
-	[[Category: Wawrzak, Z]]	+	[[Category: Yim V]]
-	[[Category: Yim, V]]	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Atp grasp domain]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Phosphohistidine domain]]~~	+
-	~~[[Category: Phosphotransferase]]~~	+
-	~~[[Category: Rifampin]]~~	+
-	~~[[Category: Rifamycin]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:09, 29 April 2020

OCA — Wed, 29 Apr 2020 08:09:29 GMT

←Older revision		Revision as of 08:09, 29 April 2020
Line 1:		Line 1:

	==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with ADP and magnesium==		==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with ADP and magnesium==
-	<StructureSection load='5fbs' size='340' side='right' caption='[[5fbs]], [[Resolution\|resolution]] 2.59Å' scene=''>	+	<StructureSection load='5fbs' size='340' side='right'caption='[[5fbs]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fbs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FBS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fbs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lismf Lismf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FBS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fbt\|5fbt]], [[5fbu\|5fbu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fbt\|5fbt]], [[5fbu\|5fbu]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbs OCA], [http://pdbe.org/5fbs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbs RCSB], [http://www.ebi.ac.uk/pdbsum/5fbs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbs OCA], [http://pdbe.org/5fbs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbs RCSB], [http://www.ebi.ac.uk/pdbsum/5fbs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fbs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lismf]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 15:49, 10 May 2016

OCA — Tue, 10 May 2016 15:49:23 GMT

←Older revision		Revision as of 15:49, 10 May 2016
Line 1:		Line 1:
		+
	==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with ADP and magnesium==		==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with ADP and magnesium==
	<StructureSection load='5fbs' size='340' side='right' caption='[[5fbs]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='5fbs' size='340' side='right' caption='[[5fbs]], [[Resolution\|resolution]] 2.59Å' scene=''>
Line 7:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbs OCA], [http://pdbe.org/5fbs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbs RCSB], [http://www.ebi.ac.uk/pdbsum/5fbs PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbs OCA], [http://pdbe.org/5fbs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbs RCSB], [http://www.ebi.ac.uk/pdbsum/5fbs PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rifampin (RIF) phosphotransferase (RPH) confers antibiotic resistance by conversion of RIF and ATP, to inactive phospho-RIF, AMP and Pi. Here we present the crystal structure of RPH from Listeria monocytogenes (RPH-Lm), which reveals that the enzyme is comprised of three domains: two substrate-binding domains (ATP-grasp and RIF-binding domains); and a smaller phosphate-carrying His swivel domain. Using solution small-angle X-ray scattering and mutagenesis, we reveal a mechanism where the swivel domain transits between the spatially distinct substrate-binding sites during catalysis. RPHs are previously uncharacterized dikinases that are widespread in environmental and pathogenic bacteria. These enzymes are members of a large unexplored group of bacterial enzymes with substrate affinities that have yet to be fully explored. Such an enzymatically complex mechanism of antibiotic resistance augments the spectrum of strategies used by bacteria to evade antimicrobial compounds.
		+
		+	Rifampin phosphotransferase is an unusual antibiotic resistance kinase.,Stogios PJ, Cox G, Spanogiannopoulos P, Pillon MC, Waglechner N, Skarina T, Koteva K, Guarne A, Savchenko A, Wright GD Nat Commun. 2016 Apr 22;7:11343. doi: 10.1038/ncomms11343. PMID:27103605<ref>PMID:27103605</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5fbs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
Line 14:		Line 26:
	[[Category: Skarina, T]]		[[Category: Skarina, T]]
	[[Category: Stogios, P J]]		[[Category: Stogios, P J]]
		+	[[Category: Wawrzak, Z]]
	[[Category: Yim, V]]		[[Category: Yim, V]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]

OCA at 20:36, 13 January 2016

OCA — Wed, 13 Jan 2016 20:36:03 GMT

←Older revision		Revision as of 20:36, 13 January 2016
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with ADP and magnesium==
-		+	<StructureSection load='5fbs' size='340' side='right' caption='[[5fbs]], [[Resolution\|resolution]] 2.59Å' scene=''>
-	~~The entry~~ 5fbs is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5fbs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FBS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FBS FirstGlance]. <br>
-	~~Authors~~: ~~Stogios~~, P.J.~~, Skarina, T~~., ~~Yim, V~~., ~~Savchenko, A~~., ~~Anderson, W~~.F., ~~Center for Structural Genomics of Infectious Diseases (CSGID)~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fbt\|5fbt]], [[5fbu\|5fbu]]</td></tr>
-	~~Description~~: ~~Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with ADP and magnesium~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fbs OCA], [http://pdbe.org/5fbs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fbs RCSB], [http://www.ebi.ac.uk/pdbsum/5fbs PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Anderson, W F]]
		+	[[Category: Structural genomic]]
	[[Category: Savchenko, A]]		[[Category: Savchenko, A]]
-	[[Category: Yim, V]]
-	[[Category: Stogios, P.J]]
	[[Category: Skarina, T]]		[[Category: Skarina, T]]
-	[[Category: ~~Anderson~~, ~~W.F~~]]	+	[[Category: Stogios, P J]]
-	[[Category: ~~Center For Structural Genomics Of Infectious Diseases (~~Csgid)]]	+	[[Category: Yim, V]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Atp grasp domain]]
		+	[[Category: Csgid]]
		+	[[Category: Phosphohistidine domain]]
		+	[[Category: Phosphotransferase]]
		+	[[Category: Rifampin]]
		+	[[Category: Rifamycin]]
		+	[[Category: Transferase]]

OCA: Protected "5fbs" [edit=sysop:move=sysop]

OCA — Wed, 23 Dec 2015 11:25:25 GMT

Protected "5fbs" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:25, 23 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fbs is ON HOLD Authors: Stogios, P.J., Skarina, T., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (...

OCA — Wed, 23 Dec 2015 11:25:24 GMT

New page: '''Unreleased structure''' The entry 5fbs is ON HOLD Authors: Stogios, P.J., Skarina, T., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (...

New page

'''Unreleased structure'''

The entry 5fbs is ON HOLD

Authors: Stogios, P.J., Skarina, T., Yim, V., Savchenko, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with ADP and magnesium
[[Category: Unreleased Structures]]
[[Category: Savchenko, A]]
[[Category: Yim, V]]
[[Category: Stogios, P.J]]
[[Category: Skarina, T]]
[[Category: Anderson, W.F]]
[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]