5fce - Revision history

OCA at 06:46, 19 July 2023

OCA — Wed, 19 Jul 2023 06:46:13 GMT

←Older revision		Revision as of 06:46, 19 July 2023
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	==The crystal structure of the ligand binding region of Serine-glutamate repeat protein A (SgrA) of Enterococcus faecium==		==The crystal structure of the ligand binding region of Serine-glutamate repeat protein A (SgrA) of Enterococcus faecium==
-	<StructureSection load='5fce' size='340' side='right' caption='[[5fce]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5fce' size='340' side='right'caption='[[5fce]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fce]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Enterococcus_faecium_atcc_baa-472~~ Enterococcus faecium ~~atcc baa-472~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FCE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FCE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fce]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium_DO Enterococcus faecium DO]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FCE FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.798Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~fms2, HMPREF0351_11523 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=333849 Enterococcus faecium ATCC BAA-472])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fce OCA], [~~http~~://pdbe.org/5fce PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fce RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fce PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fce ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fce OCA], [https://pdbe.org/5fce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fce RCSB], [https://www.ebi.ac.uk/pdbsum/5fce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fce ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q3Y373_ENTFD Q3Y373_ENTFD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus faecium ~~atcc baa-472~~]]	+	[[Category: Enterococcus faecium DO]]
-	[[Category: ~~Nagarajan, R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Ponnuraj, K~~]]	+	[[Category: Nagarajan R]]
-	[[Category: ~~Cell adhesion]]~~	+	[[Category: Ponnuraj K]]
-	~~[[Category: Flattened barrel~~]]	+

OCA at 07:31, 6 December 2017

OCA — Wed, 06 Dec 2017 07:31:42 GMT

←Older revision		Revision as of 07:31, 6 December 2017
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	<StructureSection load='5fce' size='340' side='right' caption='[[5fce]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5fce' size='340' side='right' caption='[[5fce]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fce]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FCE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fce]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium_atcc_baa-472 Enterococcus faecium atcc baa-472]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FCE FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fms2, HMPREF0351_11523 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=333849 Enterococcus faecium ATCC BAA-472])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fce OCA], [http://pdbe.org/5fce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fce RCSB], [http://www.ebi.ac.uk/pdbsum/5fce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fce ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fce OCA], [http://pdbe.org/5fce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fce RCSB], [http://www.ebi.ac.uk/pdbsum/5fce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fce ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Enterococcus faecium atcc baa-472]]
	[[Category: Nagarajan, R]]		[[Category: Nagarajan, R]]
	[[Category: Ponnuraj, K]]		[[Category: Ponnuraj, K]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Flattened barrel]]		[[Category: Flattened barrel]]

OCA at 02:21, 13 July 2016

OCA — Wed, 13 Jul 2016 02:21:33 GMT

←Older revision		Revision as of 02:21, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5fce is ~~ON HOLD until Paper~~ Publication	+	==The crystal structure of the ligand binding region of Serine-glutamate repeat protein A (SgrA) of Enterococcus faecium==
		+	<StructureSection load='5fce' size='340' side='right' caption='[[5fce]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fce]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FCE FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fce OCA], [http://pdbe.org/5fce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fce RCSB], [http://www.ebi.ac.uk/pdbsum/5fce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fce ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antimicrobial resistant and hospital-adapted Enterococcus faecium represents a clinical problem in immunocompromized patients in hospitals worldwide. Understanding the molecular pathogenesis of E. faecium infections may provide for novel therapies to treat or prevent infections. A potential target for novel treatment therapies is the serine-glutamate repeat containing protein A (SgrA), which is a cell wall-anchored LPxTG surface protein implicated in binding to fibrinogen and nidogen. Here we report the x-ray crystal structure of the N-terminal ligand binding domain of SgrA (rSgrA28-153 ) to a resolution of 1.79 A. The structure revealed a new protein fold with significant differences from previously characterized DEv-IgG- and inv-IgG-like folds of adhesive proteins known as microbial surface components recognizing adhesive matrix molecules. The structure contains a Lys-Asn-Glu triad with the potential to form a Lys-Asn isopeptide bond. However, even in the absence of a stabilizing intramolecular isopeptide bond, rSgrA28-153 exhibits remarkable properties like resistance to proteases and high thermal stability. The interaction of rSgrA28-153 with fibrinogen, nidogen, laminin and abiotic surfaces has been characterized and rSgrA28-153 binds to these molecules with high affinity. rSgrA28-153 also binds to the beta chain of fibrinogen and with high affinity and specificity. Strikingly, the presence of 29 surface-exposed hydrophobic amino acid residues likely play an important role in the selectivity of rSgrA28-153 to bind to different abiotic surfaces. The results obtained from this study have opened new avenues to explore and understand the role of this unique surface adhesin in the pathogenesis of catheter-related infections. This article is protected by copyright. All rights reserved.

-	~~Authors~~: ~~Ponnuraj, K~~., Nagarajan, R.	+	The crystal structure of the ligand binding region of Serine-glutamate repeat protein A (SgrA) of Enterococcus faecium reveals a new protein fold: functional characterization and insights into its adhesion function.,Nagarajan R, Hendrickx AP, Ponnuraj K FEBS J. 2016 Jun 23. doi: 10.1111/febs.13792. PMID:27334767<ref>PMID:27334767</ref>

-	~~Description: The crystal structure~~ of the ~~ligand binding region~~ of ~~Serine-glutamate repeat protein A (SgrA) of Enterococcus faecium~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Ponnuraj, K]]~~	+	<div class="pdbe-citations 5fce" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Nagarajan, R]]		[[Category: Nagarajan, R]]
		+	[[Category: Ponnuraj, K]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Flattened barrel]]

OCA: Protected "5fce" [edit=sysop:move=sysop]

OCA — Wed, 23 Dec 2015 11:26:49 GMT

Protected "5fce" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:26, 23 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fce is ON HOLD until Paper Publication Authors: Ponnuraj, K., Nagarajan, R. Description: The crystal structure of the ligand binding region of Ser...

OCA — Wed, 23 Dec 2015 11:26:48 GMT

New page: '''Unreleased structure''' The entry 5fce is ON HOLD until Paper Publication Authors: Ponnuraj, K., Nagarajan, R. Description: The crystal structure of the ligand binding region of Ser...

New page

'''Unreleased structure'''

The entry 5fce is ON HOLD until Paper Publication

Authors: Ponnuraj, K., Nagarajan, R.

Description: The crystal structure of the ligand binding region of Serine-glutamate repeat protein A (SgrA) of Enterococcus faecium
[[Category: Unreleased Structures]]
[[Category: Ponnuraj, K]]
[[Category: Nagarajan, R]]