5fd7 - Revision history

OCA at 06:46, 19 July 2023

OCA — Wed, 19 Jul 2023 06:46:53 GMT

←Older revision		Revision as of 06:46, 19 July 2023
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	<StructureSection load='5fd7' size='340' side='right'caption='[[5fd7]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5fd7' size='340' side='right'caption='[[5fd7]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fd7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FD7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fd7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FD7 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SNF7~~, ~~DID1, VPS32, YLR025W (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=559292 Baker's yeast])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fd7 OCA], [~~http~~://pdbe.org/5fd7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fd7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fd7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fd7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fd7 OCA], [https://pdbe.org/5fd7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fd7 RCSB], [https://www.ebi.ac.uk/pdbsum/5fd7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fd7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SNF7_YEAST SNF7_YEAST]] Required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Appears to sequester MVB cargo. Recruits BRO1, which in turn recruits DOA4, which deubiquitinates cargos before their enclosure within MVB vesicles.<ref>PMID:11559748</ref> <ref>PMID:12194857</ref>	+	[https://www.uniprot.org/uniprot/SNF7_YEAST SNF7_YEAST] Required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Appears to sequester MVB cargo. Recruits BRO1, which in turn recruits DOA4, which deubiquitinates cargos before their enclosure within MVB vesicles.<ref>PMID:11559748</ref> <ref>PMID:12194857</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Borbat~~, P P~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Buchkovich~~, N J~~]]	+	[[Category: Borbat PP]]
-	[[Category: Emr~~, S D~~]]	+	[[Category: Buchkovich NJ]]
-	[[Category: Freed~~, J H~~]]	+	[[Category: Emr SD]]
-	[[Category: Fromme~~, J C~~]]	+	[[Category: Freed JH]]
-	[[Category: Henne~~, W M~~]]	+	[[Category: Fromme JC]]
-	[[Category: Mao, Y]]	+	[[Category: Henne WM]]
-	[[Category: Tang, S]]	+	[[Category: Mao Y]]
-	~~[[Category: Active]]~~	+	[[Category: Tang S]]
-	~~[[Category: Core]]~~	+
-	~~[[Category: Escrt]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Snf7~~]]	+

OCA at 11:37, 25 December 2019

OCA — Wed, 25 Dec 2019 11:37:38 GMT

←Older revision		Revision as of 11:37, 25 December 2019
Line 1:		Line 1:

	==X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation A)==		==X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation A)==
-	<StructureSection load='5fd7' size='340' side='right' caption='[[5fd7]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5fd7' size='340' side='right'caption='[[5fd7]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5fd7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FD7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5fd7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FD7 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Borbat, P P]]		[[Category: Borbat, P P]]
	[[Category: Buchkovich, N J]]		[[Category: Buchkovich, N J]]

OCA at 17:13, 15 November 2017

OCA — Wed, 15 Nov 2017 17:13:22 GMT

←Older revision		Revision as of 17:13, 15 November 2017
Line 1:		Line 1:
		+
	==X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation A)==		==X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation A)==
	<StructureSection load='5fd7' size='340' side='right' caption='[[5fd7]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5fd7' size='340' side='right' caption='[[5fd7]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fd7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FD7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fd7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FD7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fd7 OCA], [http://pdbe.org/5fd7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fd7 RCSB], [http://www.ebi.ac.uk/pdbsum/5fd7 PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SNF7, DID1, VPS32, YLR025W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fd7 OCA], [http://pdbe.org/5fd7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fd7 RCSB], [http://www.ebi.ac.uk/pdbsum/5fd7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fd7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
	[[Category: Borbat, P P]]		[[Category: Borbat, P P]]
	[[Category: Buchkovich, N J]]		[[Category: Buchkovich, N J]]

OCA at 19:33, 30 December 2015

OCA — Wed, 30 Dec 2015 19:33:12 GMT

←Older revision		Revision as of 19:33, 30 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation A)==
		+	<StructureSection load='5fd7' size='340' side='right' caption='[[5fd7]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fd7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FD7 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fd7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fd7 OCA], [http://pdbe.org/5fd7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fd7 RCSB], [http://www.ebi.ac.uk/pdbsum/5fd7 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SNF7_YEAST SNF7_YEAST]] Required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Appears to sequester MVB cargo. Recruits BRO1, which in turn recruits DOA4, which deubiquitinates cargos before their enclosure within MVB vesicles.<ref>PMID:11559748</ref> <ref>PMID:12194857</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The endosomal sorting complexes required for transport (ESCRTs) constitute hetero-oligomeric machines that catalyze multiple topologically similar membrane-remodeling processes. Although ESCRT-III subunits polymerize into spirals, how individual ESCRT-III subunits are activated and assembled together into a membrane-deforming filament remains unknown. Here, we determine X-ray crystal structures of the most abundant ESCRT-III subunit Snf7 in its active conformation. Using pulsed dipolar electron spin resonance spectroscopy (PDS), we show that Snf7 activation requires a prominent conformational rearrangement to expose protein-membrane and protein-protein interfaces. This promotes the assembly of Snf7 arrays with ~30A periodicity into a membrane-sculpting filament. Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block ESCRT function. The architecture of the activated and membrane-bound Snf7 polymer provides crucial insights into the spatially unique ESCRT-III-mediated membrane remodeling.

-	~~The entry 5fd7 is ON HOLD~~	+	Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments.,Tang S, Henne WM, Borbat PP, Buchkovich NJ, Freed JH, Mao Y, Fromme JC, Emr SD Elife. 2015 Dec 15;4. pii: e12548. doi: 10.7554/eLife.12548. PMID:26670543<ref>PMID:26670543</ref>

-	~~Authors: Tang~~, S.~~, Henne, W~~.~~M.,~~ Borbat, P.P., Buchkovich, N.J~~., Mao, Y., Fromme, J.C.,~~ Emr, S.D.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation A)~~	+	<div class="pdbe-citations 5fd7" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: ~~Emr~~, ~~S.D~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Borbat, P P]]
		+	[[Category: Buchkovich, N J]]
		+	[[Category: Emr, S D]]
		+	[[Category: Freed, J H]]
		+	[[Category: Fromme, J C]]
		+	[[Category: Henne, W M]]
	[[Category: Mao, Y]]		[[Category: Mao, Y]]
-	[[Category: Borbat, P.P]]
	[[Category: Tang, S]]		[[Category: Tang, S]]
-	[[Category: ~~Fromme, J.C~~]]	+	[[Category: Active]]
-	[[Category: ~~Buchkovich, N.J~~]]	+	[[Category: Core]]
-	[[Category: ~~Henne, W.M~~]]	+	[[Category: Escrt]]
		+	[[Category: Protein transport]]
		+	[[Category: Snf7]]

OCA: Protected "5fd7" [edit=sysop:move=sysop]

OCA — Wed, 23 Dec 2015 11:29:27 GMT

Protected "5fd7" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:29, 23 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fd7 is ON HOLD Authors: Tang, S., Henne, W.M., Borbat, P.P., Buchkovich, N.J., Mao, Y., Fromme, J.C., Emr, S.D. Description: X-ray Crystal Structu...

OCA — Wed, 23 Dec 2015 11:29:25 GMT

New page: '''Unreleased structure''' The entry 5fd7 is ON HOLD Authors: Tang, S., Henne, W.M., Borbat, P.P., Buchkovich, N.J., Mao, Y., Fromme, J.C., Emr, S.D. Description: X-ray Crystal Structu...

New page

'''Unreleased structure'''

The entry 5fd7 is ON HOLD

Authors: Tang, S., Henne, W.M., Borbat, P.P., Buchkovich, N.J., Mao, Y., Fromme, J.C., Emr, S.D.

Description: X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation A)
[[Category: Unreleased Structures]]
[[Category: Emr, S.D]]
[[Category: Mao, Y]]
[[Category: Borbat, P.P]]
[[Category: Tang, S]]
[[Category: Fromme, J.C]]
[[Category: Buchkovich, N.J]]
[[Category: Henne, W.M]]