5fek - Revision history

OCA at 06:48, 19 July 2023

OCA — Wed, 19 Jul 2023 06:48:18 GMT

←Older revision		Revision as of 06:48, 19 July 2023
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	<StructureSection load='5fek' size='340' side='right'caption='[[5fek]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5fek' size='340' side='right'caption='[[5fek]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fek]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FEK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FEK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fek]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FEK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Lysozyme Lysozyme]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fek OCA], [~~http~~://pdbe.org/5fek PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fek RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fek PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fek ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fek OCA], [https://pdbe.org/5fek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fek RCSB], [https://www.ebi.ac.uk/pdbsum/5fek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fek ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Tomizaki T]]
-	[[Category: Tomizaki, T]]	+	[[Category: Tsujino S]]
-	[[Category: Tsujino, S]]	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:15, 6 May 2020

OCA — Wed, 06 May 2020 07:15:18 GMT

←Older revision		Revision as of 07:15, 6 May 2020
Line 1:		Line 1:

	==Hen egg lysozyme at room temperature solved from 3600 diffraction images acquired by ultrasonic acoustic levitation method and processed by CrystFEL==		==Hen egg lysozyme at room temperature solved from 3600 diffraction images acquired by ultrasonic acoustic levitation method and processed by CrystFEL==
-	<StructureSection load='5fek' size='340' side='right' caption='[[5fek]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5fek' size='340' side='right'caption='[[5fek]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fek]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FEK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FEK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fek]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FEK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FEK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fek OCA], [http://pdbe.org/5fek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fek RCSB], [http://www.ebi.ac.uk/pdbsum/5fek PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fek OCA], [http://pdbe.org/5fek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fek RCSB], [http://www.ebi.ac.uk/pdbsum/5fek PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fek ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5fek" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fek" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Tomizaki, T]]		[[Category: Tomizaki, T]]
	[[Category: Tsujino, S]]		[[Category: Tsujino, S]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 22:56, 1 June 2016

OCA — Wed, 01 Jun 2016 22:56:51 GMT

←Older revision		Revision as of 22:56, 1 June 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5fek is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Hen egg lysozyme at room temperature solved from 3600 diffraction images acquired by ultrasonic acoustic levitation method and processed by CrystFEL==
		+	<StructureSection load='5fek' size='340' side='right' caption='[[5fek]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fek]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FEK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FEK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fek OCA], [http://pdbe.org/5fek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fek RCSB], [http://www.ebi.ac.uk/pdbsum/5fek PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the structure obtained by the conventional oscillation method for up to a 1.8-A resolution limit. In particular, the absence of protein crystal damage resulting from the acoustic levitation was carefully established. These results represent a key step towards a fully automated sample handling and measurement pipeline, which has promising prospects for a high acquisition rate and high sample efficiency for room temperature X-ray crystallography.

-	~~Authors: Tsujino~~, S., Tomizaki, T.	+	Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature.,Tsujino S, Tomizaki T Sci Rep. 2016 May 6;6:25558. doi: 10.1038/srep25558. PMID:27150272<ref>PMID:27150272</ref>

-	~~Description~~: ~~Hen egg lysozyme at room temperature solved from 3600 diffraction images acquired by ultrasonic acoustic levitation method and processed by CrystFEL~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5fek" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Lysozyme]]
	[[Category: Tomizaki, T]]		[[Category: Tomizaki, T]]
	[[Category: Tsujino, S]]		[[Category: Tsujino, S]]
		+	[[Category: Hydrolase]]

OCA at 17:50, 30 December 2015

OCA — Wed, 30 Dec 2015 17:50:37 GMT

←Older revision		Revision as of 17:50, 30 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fek is ON HOLD	+	The entry 5fek is ON HOLD until Paper Publication

	Authors: Tsujino, S., Tomizaki, T.		Authors: Tsujino, S., Tomizaki, T.

OCA: Protected "5fek" [edit=sysop:move=sysop]

OCA — Wed, 23 Dec 2015 11:33:11 GMT

Protected "5fek" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:33, 23 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fek is ON HOLD Authors: Tsujino, S., Tomizaki, T. Description: Hen egg lysozyme at room temperature solved from 3600 diffraction images acquired b...

OCA — Wed, 23 Dec 2015 11:33:10 GMT

New page: '''Unreleased structure''' The entry 5fek is ON HOLD Authors: Tsujino, S., Tomizaki, T. Description: Hen egg lysozyme at room temperature solved from 3600 diffraction images acquired b...

New page

'''Unreleased structure'''

The entry 5fek is ON HOLD

Authors: Tsujino, S., Tomizaki, T.

Description: Hen egg lysozyme at room temperature solved from 3600 diffraction images acquired by ultrasonic acoustic levitation method and processed by CrystFEL
[[Category: Unreleased Structures]]
[[Category: Tomizaki, T]]
[[Category: Tsujino, S]]