5fgs - Revision history

OCA at 16:24, 8 November 2023

OCA — Wed, 08 Nov 2023 16:24:44 GMT

←Older revision		Revision as of 16:24, 8 November 2023
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	</div>		</div>
	<div class="pdbe-citations 5fgs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fgs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Pilin 3D structures\|Pilin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:50, 19 July 2023

OCA — Wed, 19 Jul 2023 06:50:30 GMT

←Older revision		Revision as of 06:50, 19 July 2023
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	<StructureSection load='5fgs' size='340' side='right'caption='[[5fgs]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5fgs' size='340' side='right'caption='[[5fgs]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fgs]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lacrg Lacrg~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FGS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FGS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fgs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacticaseibacillus_rhamnosus_GG Lacticaseibacillus rhamnosus GG]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FGS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5f44\|5f44]], [[5faa\|5faa]], [[5fgr\|5fgr]], [[5fie\|5fie]], [[5hbb\|5hbb]], [[5hdl\|5hdl]], [[5hts\|5hts]], [[5j4m\|5j4m]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">LRHM_0426 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=568703 LACRG])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fgs OCA], [https://pdbe.org/5fgs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fgs RCSB], [https://www.ebi.ac.uk/pdbsum/5fgs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fgs ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fgs OCA], [~~http~~://pdbe.org/5fgs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fgs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fgs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fgs ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lacrg~~]]	+	[[Category: Lacticaseibacillus rhamnosus GG]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chaurasia, P]]	+	[[Category: Chaurasia P]]
-	[[Category: Krishnan, V]]	+	[[Category: Krishnan V]]
-	~~[[Category: Ossowski, I von~~]]	+	[[Category: Palva A]]
-	[[Category: Palva, A]]	+	[[Category: Pratap S]]
-	[[Category: Pratap, S]]	+	[[Category: Von Ossowski I]]
-	[[Category: ~~Adhesin]]~~	+
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Isopeptide]]~~	+
-	~~[[Category: Pilin]]~~	+
-	~~[[Category: Probiotic]]~~	+
-	~~[[Category: Spaa]]~~	+
-	~~[[Category: Spacba pili~~]]	+

OCA at 07:18, 6 May 2020

OCA — Wed, 06 May 2020 07:18:52 GMT

←Older revision		Revision as of 07:18, 6 May 2020
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	==Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG - P21212 space group==		==Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG - P21212 space group==
-	<StructureSection load='5fgs' size='340' side='right' caption='[[5fgs]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5fgs' size='340' side='right'caption='[[5fgs]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fgs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FGS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FGS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fgs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacrg Lacrg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FGS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FGS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5f44\|5f44]], [[5faa\|5faa]], [[5fgr\|5fgr]], [[5fie\|5fie]], [[5hbb\|5hbb]], [[5hdl\|5hdl]], [[5hts\|5hts]], [[5j4m\|5j4m]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5f44\|5f44]], [[5faa\|5faa]], [[5fgr\|5fgr]], [[5fie\|5fie]], [[5hbb\|5hbb]], [[5hdl\|5hdl]], [[5hts\|5hts]], [[5j4m\|5j4m]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fgs OCA], [http://pdbe.org/5fgs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fgs RCSB], [http://www.ebi.ac.uk/pdbsum/5fgs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fgs ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LRHM_0426 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=568703 LACRG])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fgs OCA], [http://pdbe.org/5fgs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fgs RCSB], [http://www.ebi.ac.uk/pdbsum/5fgs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fgs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lacrg]]
		+	[[Category: Large Structures]]
	[[Category: Chaurasia, P]]		[[Category: Chaurasia, P]]
	[[Category: Krishnan, V]]		[[Category: Krishnan, V]]

OCA at 15:14, 26 July 2016

OCA — Tue, 26 Jul 2016 15:14:06 GMT

←Older revision		Revision as of 15:14, 26 July 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5fgs is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG - P21212 space group==
		+	<StructureSection load='5fgs' size='340' side='right' caption='[[5fgs]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fgs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FGS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5f44\|5f44]], [[5faa\|5faa]], [[5fgr\|5fgr]], [[5fie\|5fie]], [[5hbb\|5hbb]], [[5hdl\|5hdl]], [[5hts\|5hts]], [[5j4m\|5j4m]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fgs OCA], [http://pdbe.org/5fgs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fgs RCSB], [http://www.ebi.ac.uk/pdbsum/5fgs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fgs ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thus far, all solved structures of pilin-proteins comprising sortase-assembled pili are from pathogenic genera and species. Here, we present the first crystal structure of a pilin subunit (SpaA) from a non-pathogen host (Lactobacillus rhamnosus GG). SpaA consists of two tandem CnaB-type domains, each with an isopeptide bond and E-box motif. Intriguingly, while the isopeptide bond in the N-terminal domain forms between lysine and asparagine, the one in the C-terminal domain atypically involves aspartate. We also solved crystal structures of mutant proteins where residues implicated in forming isopeptide bonds were replaced. Expectedly, the E-box-substituted E139A mutant lacks an isopeptide bond in the N-terminal domain. However, the C-terminal E269A substitution gave two structures; one of both domains with their isopeptide bonds present, and another of only the N-terminal domain, but with an unformed isopeptide bond and significant conformational changes. This latter crystal structure has never been observed for any other Gram-positive pilin. Notably, the C-terminal isopeptide bond still forms in D295N-substituted SpaA, irrespective of E269 being present or absent. Although E-box mutations affect SpaA proteolytic and thermal stability, a cumulative effect perturbing normal pilus polymerization was unobserved. A model showing the polymerized arrangement of SpaA within the SpaCBA pilus is proposed.

-	~~Authors: Chaurasia~~, P., Pratap, S., von Ossowski, I., Palva, A., Krishnan, V.	+	New insights about pilus formation in gut-adapted Lactobacillus rhamnosus GG from the crystal structure of the SpaA backbone-pilin subunit.,Chaurasia P, Pratap S, von Ossowski I, Palva A, Krishnan V Sci Rep. 2016 Jun 28;6:28664. doi: 10.1038/srep28664. PMID:27349405<ref>PMID:27349405</ref>

-	~~Description: Crystal structure~~ of ~~C-terminal domain~~ of ~~shaft pilin spaA from Lactobacillus rhamnosus GG -P21212 space group~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Pratap, S]]~~	+	<div class="pdbe-citations 5fgs" style="background-color:#fffaf0;"></div>
-	~~[[Category: Krishnan, V]]~~	+	== References ==
-	~~[[Category: Von Ossowski, I]]~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Chaurasia, P]]		[[Category: Chaurasia, P]]
		+	[[Category: Krishnan, V]]
		+	[[Category: Ossowski, I von]]
	[[Category: Palva, A]]		[[Category: Palva, A]]
		+	[[Category: Pratap, S]]
		+	[[Category: Adhesin]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Isopeptide]]
		+	[[Category: Pilin]]
		+	[[Category: Probiotic]]
		+	[[Category: Spaa]]
		+	[[Category: Spacba pili]]

OCA at 22:55, 10 May 2016

OCA — Tue, 10 May 2016 22:55:36 GMT

←Older revision		Revision as of 22:55, 10 May 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fgs is ON HOLD	+	The entry 5fgs is ON HOLD until Paper Publication

	Authors: Chaurasia, P., Pratap, S., von Ossowski, I., Palva, A., Krishnan, V.		Authors: Chaurasia, P., Pratap, S., von Ossowski, I., Palva, A., Krishnan, V.

OCA: Protected "5fgs" [edit=sysop:move=sysop]

OCA — Wed, 30 Dec 2015 17:55:41 GMT

Protected "5fgs" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:55, 30 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fgs is ON HOLD Authors: Chaurasia, P., Pratap, S., von Ossowski, I., Palva, A., Krishnan, V. Description: Crystal structure of C-terminal domain o...

OCA — Wed, 30 Dec 2015 17:55:40 GMT

New page: '''Unreleased structure''' The entry 5fgs is ON HOLD Authors: Chaurasia, P., Pratap, S., von Ossowski, I., Palva, A., Krishnan, V. Description: Crystal structure of C-terminal domain o...

New page

'''Unreleased structure'''

The entry 5fgs is ON HOLD

Authors: Chaurasia, P., Pratap, S., von Ossowski, I., Palva, A., Krishnan, V.

Description: Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG -P21212 space group
[[Category: Unreleased Structures]]
[[Category: Pratap, S]]
[[Category: Krishnan, V]]
[[Category: Von Ossowski, I]]
[[Category: Chaurasia, P]]
[[Category: Palva, A]]