5fhr - Revision history

OCA at 06:51, 19 July 2023

OCA — Wed, 19 Jul 2023 06:51:35 GMT

←Older revision		Revision as of 06:51, 19 July 2023
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	<StructureSection load='5fhr' size='340' side='right'caption='[[5fhr]], [[Resolution\|resolution]] 1.63Å' scene=''>		<StructureSection load='5fhr' size='340' side='right'caption='[[5fhr]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fhr]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FHR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FHR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fhr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FHR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=DNC:3,5-DINITROCATECHOL'>DNC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.63Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Comt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=DNC:3,5-DINITROCATECHOL'>DNC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catechol_O-methyltransferase Catechol O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.6 2.1.1.6] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fhr OCA], [https://pdbe.org/5fhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fhr RCSB], [https://www.ebi.ac.uk/pdbsum/5fhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fhr ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fhr OCA], [~~http~~://pdbe.org/5fhr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fhr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fhr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fhr ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/COMT_RAT COMT_RAT]] Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.	+	[https://www.uniprot.org/uniprot/COMT_RAT COMT_RAT] Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
-	[[Category: Catechol O-methyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Levy, C~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Methyltransferase regioselectivity]]~~	+	[[Category: Levy C]]
-	~~[[Category: Transferase~~]]	+

OCA at 07:20, 6 May 2020

OCA — Wed, 06 May 2020 07:20:24 GMT

←Older revision		Revision as of 07:20, 6 May 2020
Line 1:		Line 1:

	==Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol==		==Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol==
-	<StructureSection load='5fhr' size='340' side='right' caption='[[5fhr]], [[Resolution\|resolution]] 1.63Å' scene=''>	+	<StructureSection load='5fhr' size='340' side='right'caption='[[5fhr]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fhr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FHR OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FHR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fhr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FHR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FHR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=DNC:3,5-DINITROCATECHOL'>DNC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=DNC:3,5-DINITROCATECHOL'>DNC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Comt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catechol_O-methyltransferase Catechol O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.6 2.1.1.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catechol_O-methyltransferase Catechol O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.6 2.1.1.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fhr OCA], [http://pdbe.org/5fhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fhr RCSB], [http://www.ebi.ac.uk/pdbsum/5fhr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fhr OCA], [http://pdbe.org/5fhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fhr RCSB], [http://www.ebi.ac.uk/pdbsum/5fhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fhr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5fhr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fhr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Catechol O-methyltransferase 3D structures\|Catechol O-methyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buffalo rat]]
	[[Category: Catechol O-methyltransferase]]		[[Category: Catechol O-methyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Levy, C]]		[[Category: Levy, C]]
	[[Category: Methyltransferase regioselectivity]]		[[Category: Methyltransferase regioselectivity]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:14, 26 February 2016

OCA — Fri, 26 Feb 2016 10:14:53 GMT

←Older revision		Revision as of 10:14, 26 February 2016
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		+
	==Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol==		==Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol==
	<StructureSection load='5fhr' size='340' side='right' caption='[[5fhr]], [[Resolution\|resolution]] 1.63Å' scene=''>		<StructureSection load='5fhr' size='340' side='right' caption='[[5fhr]], [[Resolution\|resolution]] 1.63Å' scene=''>

OCA at 15:37, 3 February 2016

OCA — Wed, 03 Feb 2016 15:37:28 GMT

←Older revision		Revision as of 15:37, 3 February 2016
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol==
		+	<StructureSection load='5fhr' size='340' side='right' caption='[[5fhr]], [[Resolution\|resolution]] 1.63Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fhr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FHR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FHR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=DNC:3,5-DINITROCATECHOL'>DNC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catechol_O-methyltransferase Catechol O-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.6 2.1.1.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fhr OCA], [http://pdbe.org/5fhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fhr RCSB], [http://www.ebi.ac.uk/pdbsum/5fhr PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/COMT_RAT COMT_RAT]] Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Catechol-O-methyltransferase (COMT), an important therapeutic target in the treatment of Parkinson's disease, is also being developed for biocatalytic processes, including vanillin production, although lack of regioselectivity has precluded its more widespread application. By using structural and mechanistic information, regiocomplementary COMT variants were engineered that deliver either meta- or para-methylated catechols. X-ray crystallography further revealed how the active-site residues and quaternary structure govern regioselectivity. Finally, analogues of AdoMet are accepted by the regiocomplementary COMT mutants and can be used to prepare alkylated catechols, including ethyl vanillin.

-	~~The entry 5fhr is ON HOLD~~	+	Effects of Active-Site Modification and Quaternary Structure on the Regioselectivity of Catechol-O-Methyltransferase.,Law BJ, Bennett MR, Thompson ML, Levy C, Shepherd SA, Leys D, Micklefield J Angew Chem Int Ed Engl. 2016 Jan 21. doi: 10.1002/anie.201508287. PMID:26797714<ref>PMID:26797714</ref>

-	~~Authors: Levy~~, C.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of Y200L mutant of Rat Catechol~~-O-~~Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol~~	+	<div class="pdbe-citations 5fhr" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Catechol O-methyltransferase]]
	[[Category: Levy, C]]		[[Category: Levy, C]]
		+	[[Category: Methyltransferase regioselectivity]]
		+	[[Category: Transferase]]

OCA: Protected "5fhr" [edit=sysop:move=sysop]

OCA — Wed, 30 Dec 2015 17:58:33 GMT

Protected "5fhr" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:58, 30 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fhr is ON HOLD Authors: Levy, C. Description: Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5...

OCA — Wed, 30 Dec 2015 17:58:32 GMT

New page: '''Unreleased structure''' The entry 5fhr is ON HOLD Authors: Levy, C. Description: Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5...

New page

'''Unreleased structure'''

The entry 5fhr is ON HOLD

Authors: Levy, C.

Description: Crystal structure of Y200L mutant of Rat Catechol-O-Methyltransferase in complex with AdoMet and 3,5-dinitrocatechol
[[Category: Unreleased Structures]]
[[Category: Levy, C]]