5fi1 - Revision history

OCA at 06:51, 19 July 2023

OCA — Wed, 19 Jul 2023 06:51:48 GMT

←Older revision		Revision as of 06:51, 19 July 2023
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	<StructureSection load='5fi1' size='340' side='right'caption='[[5fi1]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5fi1' size='340' side='right'caption='[[5fi1]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fi1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FI1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FI1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fi1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FI1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FI1 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5d27\|5d27]], [[5d3v\|5d3v]], [[5d3w\|5d3w]], [[5d3x\|5d3x]], [[5d3y\|5d3y]], [[5fi0\|5fi0]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.203Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">PREX1, KIAA1415 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN]), CDC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fi1 OCA], [https://pdbe.org/5fi1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fi1 RCSB], [https://www.ebi.ac.uk/pdbsum/5fi1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fi1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fi1 OCA], [~~http~~://pdbe.org/5fi1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fi1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fi1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fi1 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PREX1_HUMAN PREX1_HUMAN]] Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils. [[http://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN]] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>	+	[https://www.uniprot.org/uniprot/PREX1_HUMAN PREX1_HUMAN] Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cash~~, J N~~]]	+	[[Category: Cash JN]]
-	[[Category: Tesmer~~, J J.G]]~~	+	[[Category: Tesmer JJG]]
-	~~[[Category: Dbl homology domain]]~~	+
-	~~[[Category: Gtpase]]~~	+
-	~~[[Category: Pleckstrin homology domain]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Rhogef]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 09:04, 4 December 2019

OCA — Wed, 04 Dec 2019 09:04:20 GMT

←Older revision		Revision as of 09:04, 4 December 2019
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	==Crystal Structure of the P-Rex1 DH/PH tandem in complex with Cdc42==		==Crystal Structure of the P-Rex1 DH/PH tandem in complex with Cdc42==
-	<StructureSection load='5fi1' size='340' side='right' caption='[[5fi1]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='5fi1' size='340' side='right'caption='[[5fi1]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fi1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FI1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fi1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FI1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d27\|5d27]], [[5d3v\|5d3v]], [[5d3w\|5d3w]], [[5d3x\|5d3x]], [[5d3y\|5d3y]], [[5fi0\|5fi0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d27\|5d27]], [[5d3v\|5d3v]], [[5d3w\|5d3w]], [[5d3x\|5d3x]], [[5d3y\|5d3y]], [[5fi0\|5fi0]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PREX1, KIAA1415 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CDC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fi1 OCA], [http://pdbe.org/5fi1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fi1 RCSB], [http://www.ebi.ac.uk/pdbsum/5fi1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fi1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fi1 OCA], [http://pdbe.org/5fi1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fi1 RCSB], [http://www.ebi.ac.uk/pdbsum/5fi1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fi1 ProSAT]</span></td></tr>
	</table>		</table>
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5fi1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fi1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Cash, J N]]		[[Category: Cash, J N]]
	[[Category: Tesmer, J J.G]]		[[Category: Tesmer, J J.G]]

OCA at 04:44, 21 September 2017

OCA — Thu, 21 Sep 2017 04:44:11 GMT

←Older revision		Revision as of 04:44, 21 September 2017
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[5fi1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FI1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5fi1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FI1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d27\|5d27]], [[5d3v\|5d3v]], [[5d3w\|5d3w]], [[5d3x\|5d3x]], [[5d3y\|5d3y]], [[5fi0\|5fi0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d27\|5d27]], [[5d3v\|5d3v]], [[5d3w\|5d3w]], [[5d3x\|5d3x]], [[5d3y\|5d3y]], [[5fi0\|5fi0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fi1 OCA], [http://pdbe.org/5fi1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fi1 RCSB], [http://www.ebi.ac.uk/pdbsum/5fi1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fi1 OCA], [http://pdbe.org/5fi1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fi1 RCSB], [http://www.ebi.ac.uk/pdbsum/5fi1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fi1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:33, 1 June 2016

OCA — Wed, 01 Jun 2016 08:33:19 GMT

←Older revision		Revision as of 08:33, 1 June 2016
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PREX1_HUMAN PREX1_HUMAN]] Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils. [[http://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN]] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>		[[http://www.uniprot.org/uniprot/PREX1_HUMAN PREX1_HUMAN]] Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils. [[http://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN]] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger 1 (P-Rex1) is a Rho guanine nucleotide exchange factor synergistically activated by PIP3 and Gbetagamma that plays an important role in the metastasis of breast, prostate, and skin cancer, making it an attractive therapeutic target. However, the molecular mechanisms behind P-Rex1 regulation are poorly understood. We determined structures of the P-Rex1 pleckstrin homology (PH) domain bound to the headgroup of PIP3 and resolved that PIP3 binding to the PH domain is required for P-Rex1 activity in cells but not for membrane localization, which points to an allosteric activation mechanism by PIP3. We also determined structures of the P-Rex1 tandem Dbl homology/PH domains in complexes with two of its substrate GTPases, Rac1 and Cdc42. Collectively, this study provides important molecular insights into P-Rex1 regulation and tools for targeting the PIP3-binding pocket of P-Rex1 with a new generation of cancer chemotherapeutic agents.
		+
		+	Structural and Biochemical Characterization of the Catalytic Core of the Metastatic Factor P-Rex1 and Its Regulation by PtdIns(3,4,5)P3.,Cash JN, Davis EM, Tesmer JJ Structure. 2016 May 3;24(5):730-40. doi: 10.1016/j.str.2016.02.022. Epub 2016 Apr, 14. PMID:27150042<ref>PMID:27150042</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5fi1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:23, 10 May 2016

OCA — Tue, 10 May 2016 17:23:01 GMT

←Older revision		Revision as of 17:23, 10 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5fi1 is ~~ON HOLD until sometime in~~ the ~~future~~	+	==Crystal Structure of the P-Rex1 DH/PH tandem in complex with Cdc42==
-		+	<StructureSection load='5fi1' size='340' side='right' caption='[[5fi1]], [[Resolution\|resolution]] 3.20Å' scene=''>
-	~~Authors~~: ~~Cash~~, J.N., ~~Tesmer~~, J.J.G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5fi1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FI1 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5d27\|5d27]], [[5d3v\|5d3v]], [[5d3w\|5d3w]], [[5d3x\|5d3x]], [[5d3y\|5d3y]], [[5fi0\|5fi0]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fi1 OCA], [http://pdbe.org/5fi1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fi1 RCSB], [http://www.ebi.ac.uk/pdbsum/5fi1 PDBsum]</span></td></tr>
-	[[Category: Tesmer, J.J.G]]	+	</table>
-	[[Category: ~~Cash, J.N~~]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PREX1_HUMAN PREX1_HUMAN]] Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils. [[http://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN]] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cash, J N]]
		+	[[Category: Tesmer, J J.G]]
		+	[[Category: Dbl homology domain]]
		+	[[Category: Gtpase]]
		+	[[Category: Pleckstrin homology domain]]
		+	[[Category: Protein binding]]
		+	[[Category: Rhogef]]
		+	[[Category: Signaling protein]]

OCA at 15:12, 26 February 2016

OCA — Fri, 26 Feb 2016 15:12:51 GMT

←Older revision		Revision as of 15:12, 26 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fi1 is ON HOLD until ~~Paper Publication~~	+	The entry 5fi1 is ON HOLD until sometime in the future

	Authors: Cash, J.N., Tesmer, J.J.G.		Authors: Cash, J.N., Tesmer, J.J.G.

OCA at 14:33, 10 February 2016

OCA — Wed, 10 Feb 2016 14:33:24 GMT

←Older revision		Revision as of 14:33, 10 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fi1 is ON HOLD	+	The entry 5fi1 is ON HOLD until Paper Publication

	Authors: Cash, J.N., Tesmer, J.J.G.		Authors: Cash, J.N., Tesmer, J.J.G.

OCA: Protected "5fi1" [edit=sysop:move=sysop]

OCA — Wed, 30 Dec 2015 17:59:39 GMT

Protected "5fi1" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:59, 30 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fi1 is ON HOLD Authors: Cash, J.N., Tesmer, J.J.G. Description: Category: Unreleased Structures Category: Tesmer, J.J.G [[Category: Cash,...

OCA — Wed, 30 Dec 2015 17:59:38 GMT

New page: '''Unreleased structure''' The entry 5fi1 is ON HOLD Authors: Cash, J.N., Tesmer, J.J.G. Description: Category: Unreleased Structures Category: Tesmer, J.J.G [[Category: Cash,...

New page

'''Unreleased structure'''

The entry 5fi1 is ON HOLD

Authors: Cash, J.N., Tesmer, J.J.G.

Description:
[[Category: Unreleased Structures]]
[[Category: Tesmer, J.J.G]]
[[Category: Cash, J.N]]