5fj6 - Revision history

OCA at 06:53, 19 July 2023

OCA — Wed, 19 Jul 2023 06:53:29 GMT

←Older revision		Revision as of 06:53, 19 July 2023
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	<SX load='5fj6' size='340' side='right' viewer='molstar' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>		<SX load='5fj6' size='340' side='right' viewer='molstar' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fj6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FJ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fj6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FJ6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 7.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5fj5\|5fj5]], [[5fj7\|5fj7]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj6 OCA], [~~http~~://pdbe.org/5fj6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fj6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fj6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fj6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj6 OCA], [https://pdbe.org/5fj6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fj6 RCSB], [https://www.ebi.ac.uk/pdbsum/5fj6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fj6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RDRP_BPPH6 RDRP_BPPH6]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Huiskonen~~, J T~~]]	+	[[Category: Pseudomonas virus phi6]]
-	[[Category: Ilca, S]]	+	[[Category: Huiskonen JT]]
-	[[Category: Kotecha, A]]	+	[[Category: Ilca S]]
-	[[Category: Poranen~~, M P~~]]	+	[[Category: Kotecha A]]
-	[[Category: Stuart~~, D I~~]]	+	[[Category: Poranen MP]]
-	[[Category: Sun, X]]	+	[[Category: Stuart DI]]
-	~~[[Category: Bacteriophage phi6]]~~	+	[[Category: Sun X]]
-	~~[[Category: P2]]~~	+
-	~~[[Category: Polymerase]]~~	+
-	~~[[Category: Polymerase complex]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 19:49, 10 April 2020

OCA — Fri, 10 Apr 2020 19:49:00 GMT

←Older revision		Revision as of 19:49, 10 April 2020
Line 3:		Line 3:
	<SX load='5fj6' size='340' side='right' viewer='molstar' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>		<SX load='5fj6' size='340' side='right' viewer='molstar' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fj6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FJ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fj6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FJ6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fj5\|5fj5]], [[5fj7\|5fj7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fj5\|5fj5]], [[5fj7\|5fj7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj6 OCA], [http://pdbe.org/5fj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fj6 RCSB], [http://www.ebi.ac.uk/pdbsum/5fj6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fj6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj6 OCA], [http://pdbe.org/5fj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fj6 RCSB], [http://www.ebi.ac.uk/pdbsum/5fj6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fj6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:13, 6 March 2020

OCA — Fri, 06 Mar 2020 18:13:08 GMT

←Older revision		Revision as of 18:13, 6 March 2020
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	==Structure of the P2 polymerase inside in vitro assembled bacteriophage phi6 polymerase complex==		==Structure of the P2 polymerase inside in vitro assembled bacteriophage phi6 polymerase complex==
-	<~~StructureSection~~ load='5fj6' size='340' side='right' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>	+	<SX load='5fj6' size='340' side='right' viewer='molstar' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5fj6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FJ6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5fj6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FJ6 FirstGlance]. <br>
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 5fj6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fj6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
		+	[[Category: Large Structures]]
	[[Category: Huiskonen, J T]]		[[Category: Huiskonen, J T]]
	[[Category: Ilca, S]]		[[Category: Ilca, S]]

OCA at 04:13, 4 August 2017

OCA — Fri, 04 Aug 2017 04:13:17 GMT

←Older revision		Revision as of 04:13, 4 August 2017
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fj5\|5fj5]], [[5fj7\|5fj7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fj5\|5fj5]], [[5fj7\|5fj7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj6 OCA], [http://pdbe.org/5fj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fj6 RCSB], [http://www.ebi.ac.uk/pdbsum/5fj6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj6 OCA], [http://pdbe.org/5fj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fj6 RCSB], [http://www.ebi.ac.uk/pdbsum/5fj6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fj6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:37, 11 May 2016

OCA — Wed, 11 May 2016 05:37:36 GMT

←Older revision		Revision as of 05:37, 11 May 2016
Line 1:		Line 1:
		+
	==Structure of the P2 polymerase inside in vitro assembled bacteriophage phi6 polymerase complex==		==Structure of the P2 polymerase inside in vitro assembled bacteriophage phi6 polymerase complex==
	<StructureSection load='5fj6' size='340' side='right' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>		<StructureSection load='5fj6' size='340' side='right' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>

OCA at 19:47, 30 November 2015

OCA — Mon, 30 Nov 2015 19:47:10 GMT

←Older revision		Revision as of 19:47, 30 November 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of the P2 polymerase inside in vitro assembled bacteriophage phi6 polymerase complex==
		+	<StructureSection load='5fj6' size='340' side='right' caption='[[5fj6]], [[Resolution\|resolution]] 7.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fj6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FJ6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fj5\|5fj5]], [[5fj7\|5fj7]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fj6 OCA], [http://pdbe.org/5fj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fj6 RCSB], [http://www.ebi.ac.uk/pdbsum/5fj6 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Electron cryomicroscopy can yield near-atomic resolution structures of highly ordered macromolecular complexes. Often however some subunits bind in a flexible manner, have different symmetry from the rest of the complex, or are present in sub-stoichiometric amounts, limiting the attainable resolution. Here we report a general method for the localized three-dimensional reconstruction of such subunits. After determining the particle orientations, local areas corresponding to the subunits can be extracted and treated as single particles. We demonstrate the method using three examples including a flexible assembly and complexes harbouring subunits with either partial occupancy or mismatched symmetry. Most notably, the method allows accurate fitting of the monomeric RNA-dependent RNA polymerase bound at the threefold axis of symmetry inside a viral capsid, revealing for the first time its exact orientation and interactions with the capsid proteins. Localized reconstruction is expected to provide novel biological insights in a range of challenging biological systems.

-	~~The entry 5fj6 is ON HOLD until Paper Publication~~	+	Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes.,Ilca SL, Kotecha A, Sun X, Poranen MM, Stuart DI, Huiskonen JT Nat Commun. 2015 Nov 4;6:8843. doi: 10.1038/ncomms9843. PMID:26534841<ref>PMID:26534841</ref>

-	~~Authors: ILCA~~, S.~~, KOTECHA, A., SUN, X., PORANEN, M.P., STUART, D.I., HUISKONEN, J.T~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of the P2 polymerase inside in vitro assembled bacteriophage phi6 polymerase complex~~	+	<div class="pdbe-citations 5fj6" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Huiskonen, J T]]
		+	[[Category: Ilca, S]]
	[[Category: Kotecha, A]]		[[Category: Kotecha, A]]
-	~~[[Category: Huiskonen, J.T]]~~	+	[[Category: Poranen, M P]]
-	[[Category: Poranen, M.P]]	+	[[Category: Stuart, D I]]
-	~~[[Category: Ilca, S~~]]	+
-	[[Category: Stuart, D.I]]	+
	[[Category: Sun, X]]		[[Category: Sun, X]]
		+	[[Category: Bacteriophage phi6]]
		+	[[Category: P2]]
		+	[[Category: Polymerase]]
		+	[[Category: Polymerase complex]]
		+	[[Category: Transcription]]

OCA at 04:37, 22 October 2015

OCA — Thu, 22 Oct 2015 04:37:18 GMT

←Older revision		Revision as of 04:37, 22 October 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fj6 is ON HOLD	+	The entry 5fj6 is ON HOLD until Paper Publication

	Authors: ILCA, S., KOTECHA, A., SUN, X., PORANEN, M.P., STUART, D.I., HUISKONEN, J.T.		Authors: ILCA, S., KOTECHA, A., SUN, X., PORANEN, M.P., STUART, D.I., HUISKONEN, J.T.

OCA: Protected "5fj6" [edit=sysop:move=sysop]

OCA — Fri, 16 Oct 2015 03:05:40 GMT

Protected "5fj6" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:05, 16 October 2015

OCA: New page: '''Unreleased structure''' The entry 5fj6 is ON HOLD Authors: ILCA, S., KOTECHA, A., SUN, X., PORANEN, M.P., STUART, D.I., HUISKONEN, J.T. Description: Structure of the P2 polymerase i...

OCA — Fri, 16 Oct 2015 03:05:39 GMT

New page: '''Unreleased structure''' The entry 5fj6 is ON HOLD Authors: ILCA, S., KOTECHA, A., SUN, X., PORANEN, M.P., STUART, D.I., HUISKONEN, J.T. Description: Structure of the P2 polymerase i...

New page

'''Unreleased structure'''

The entry 5fj6 is ON HOLD

Authors: ILCA, S., KOTECHA, A., SUN, X., PORANEN, M.P., STUART, D.I., HUISKONEN, J.T.

Description: Structure of the P2 polymerase inside in vitro assembled bacteriophage phi6 polymerase complex
[[Category: Unreleased Structures]]
[[Category: Kotecha, A]]
[[Category: Huiskonen, J.T]]
[[Category: Poranen, M.P]]
[[Category: Ilca, S]]
[[Category: Stuart, D.I]]
[[Category: Sun, X]]