5fjv - Revision history

OCA at 06:54, 19 July 2023

OCA — Wed, 19 Jul 2023 06:54:22 GMT

←Older revision		Revision as of 06:54, 19 July 2023
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	<StructureSection load='5fjv' size='340' side='right'caption='[[5fjv]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='5fjv' size='340' side='right'caption='[[5fjv]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fjv]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fjv]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FJV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPJ:EPIBATIDINE'>EPJ</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fjv OCA], [~~http~~://pdbe.org/5fjv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fjv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fjv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fjv ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPJ:EPIBATIDINE'>EPJ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fjv OCA], [https://pdbe.org/5fjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fjv RCSB], [https://www.ebi.ac.uk/pdbsum/5fjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fjv ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/ACHA2_HUMAN ACHA2_HUMAN]] Autosomal dominant nocturnal frontal lobe epilepsy. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/ACHA2_HUMAN ACHA2_HUMAN] Autosomal dominant nocturnal frontal lobe epilepsy. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ACHA2_HUMAN ACHA2_HUMAN]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.	+	[https://www.uniprot.org/uniprot/ACHA2_HUMAN ACHA2_HUMAN] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 5fjv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fjv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acetyl choline receptor 3D structures\|Acetyl choline receptor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chroni-Tzartou, D]]	+	[[Category: Chroni-Tzartou D]]
-	[[Category: Giastas, P]]	+	[[Category: Giastas P]]
-	[[Category: Kouvatsos, N]]	+	[[Category: Kouvatsos N]]
-	[[Category: Tzartos~~, S J]]~~	+	[[Category: Tzartos SJ]]
-	~~[[Category: Acetylcholine-binding protein]]~~	+
-	~~[[Category: Agonist]]~~	+
-	~~[[Category: Alpha2]]~~	+
-	~~[[Category: Epibatidine]]~~	+
-	~~[[Category: Extracellular domain]]~~	+
-	~~[[Category: Nachr]]~~	+
-	~~[[Category: Nicotinic acetylcholine receptor~~]]	+

OCA at 07:23, 6 May 2020

OCA — Wed, 06 May 2020 07:23:01 GMT

←Older revision		Revision as of 07:23, 6 May 2020
Line 1:		Line 1:

	==Crystal structure of the extracellular domain of alpha2 nicotinic acetylcholine receptor in pentameric assembly==		==Crystal structure of the extracellular domain of alpha2 nicotinic acetylcholine receptor in pentameric assembly==
-	<StructureSection load='5fjv' size='340' side='right' caption='[[5fjv]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='5fjv' size='340' side='right'caption='[[5fjv]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fjv]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fjv]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FJV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPJ:EPIBATIDINE'>EPJ</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPJ:EPIBATIDINE'>EPJ</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fjv OCA], [http://pdbe.org/5fjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fjv RCSB], [http://www.ebi.ac.uk/pdbsum/5fjv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fjv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fjv OCA], [http://pdbe.org/5fjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fjv RCSB], [http://www.ebi.ac.uk/pdbsum/5fjv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fjv ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Chroni-Tzartou, D]]		[[Category: Chroni-Tzartou, D]]
	[[Category: Giastas, P]]		[[Category: Giastas, P]]

OCA at 05:10, 9 September 2016

OCA — Fri, 09 Sep 2016 05:10:51 GMT

←Older revision		Revision as of 05:10, 9 September 2016
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ACHA2_HUMAN ACHA2_HUMAN]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.		[[http://www.uniprot.org/uniprot/ACHA2_HUMAN ACHA2_HUMAN]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In this study we report the X-ray crystal structure of the extracellular domain (ECD) of the human neuronal alpha2 nicotinic acetylcholine receptor (nAChR) subunit in complex with the agonist epibatidine at 3.2 A. Interestingly, alpha2 was crystallized as a pentamer, revealing the intersubunit interactions in a wild type neuronal nAChR ECD and the full ligand binding pocket conferred by two adjacent alpha subunits. The pentameric assembly presents the conserved structural scaffold observed in homologous proteins, as well as distinctive features, providing unique structural information of the binding site between principal and complementary faces. Structure-guided mutagenesis and electrophysiological data confirmed the presence of the alpha2(+)/alpha2(-) binding site on the heteromeric low sensitivity alpha2beta2 nAChR and validated the functional importance of specific residues in alpha2 and beta2 nAChR subunits. Given the pathological importance of the alpha2 nAChR subunit and the high sequence identity with alpha4 (78%) and other neuronal nAChR subunits, our findings offer valuable information for modeling several nAChRs and ultimately for structure-based design of subtype specific drugs against the nAChR associated diseases.
		+
		+	Crystal structure of a human neuronal nAChR extracellular domain in pentameric assembly: Ligand-bound alpha2 homopentamer.,Kouvatsos N, Giastas P, Chroni-Tzartou D, Poulopoulou C, Tzartos SJ Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9635-40. doi:, 10.1073/pnas.1602619113. Epub 2016 Aug 4. PMID:27493220<ref>PMID:27493220</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5fjv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:24, 10 August 2016

OCA — Wed, 10 Aug 2016 16:24:13 GMT

←Older revision		Revision as of 16:24, 10 August 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5fjv is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the extracellular domain of alpha2 nicotinic acetylcholine receptor in pentameric assembly==
-		+	<StructureSection load='5fjv' size='340' side='right' caption='[[5fjv]], [[Resolution\|resolution]] 3.20Å' scene=''>
-	~~Authors~~: ~~Giastas, P~~.~~, Kouvatsos, N~~., ~~Chroni~~-~~Tzartou~~, D., ~~Tzartos~~, S.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5fjv]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FJV FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of~~ the ~~extracellular domain of alpha2 nicotinic acetylcholine receptor~~ in ~~pentameric assembly~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPJ:EPIBATIDINE'>EPJ</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fjv OCA], [http://pdbe.org/5fjv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fjv RCSB], [http://www.ebi.ac.uk/pdbsum/5fjv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fjv ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/ACHA2_HUMAN ACHA2_HUMAN]] Autosomal dominant nocturnal frontal lobe epilepsy. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACHA2_HUMAN ACHA2_HUMAN]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
		+	__TOC__
		+	</StructureSection>
	[[Category: Chroni-Tzartou, D]]		[[Category: Chroni-Tzartou, D]]
	[[Category: Giastas, P]]		[[Category: Giastas, P]]
	[[Category: Kouvatsos, N]]		[[Category: Kouvatsos, N]]
-	[[Category: Tzartos, S.J]]	+	[[Category: Tzartos, S J]]
		+	[[Category: Acetylcholine-binding protein]]
		+	[[Category: Agonist]]
		+	[[Category: Alpha2]]
		+	[[Category: Epibatidine]]
		+	[[Category: Extracellular domain]]
		+	[[Category: Nachr]]
		+	[[Category: Nicotinic acetylcholine receptor]]

OCA at 06:03, 14 July 2016

OCA — Thu, 14 Jul 2016 06:03:24 GMT

←Older revision		Revision as of 06:03, 14 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fjv is ON HOLD	+	The entry 5fjv is ON HOLD until Paper Publication

	Authors: Giastas, P., Kouvatsos, N., Chroni-Tzartou, D., Tzartos, S.J.		Authors: Giastas, P., Kouvatsos, N., Chroni-Tzartou, D., Tzartos, S.J.

OCA: Protected "5fjv" [edit=sysop:move=sysop]

OCA — Thu, 22 Oct 2015 04:39:05 GMT

Protected "5fjv" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:39, 22 October 2015

OCA: New page: '''Unreleased structure''' The entry 5fjv is ON HOLD Authors: Giastas, P., Kouvatsos, N., Chroni-Tzartou, D., Tzartos, S.J. Description: Crystal structure of the extracellular domain o...

OCA — Thu, 22 Oct 2015 04:39:04 GMT

New page: '''Unreleased structure''' The entry 5fjv is ON HOLD Authors: Giastas, P., Kouvatsos, N., Chroni-Tzartou, D., Tzartos, S.J. Description: Crystal structure of the extracellular domain o...

New page

'''Unreleased structure'''

The entry 5fjv is ON HOLD

Authors: Giastas, P., Kouvatsos, N., Chroni-Tzartou, D., Tzartos, S.J.

Description: Crystal structure of the extracellular domain of alpha2 nicotinic acetylcholine receptor in pentameric assembly
[[Category: Unreleased Structures]]
[[Category: Chroni-Tzartou, D]]
[[Category: Giastas, P]]
[[Category: Kouvatsos, N]]
[[Category: Tzartos, S.J]]