5fkn - Revision history

OCA at 06:55, 19 July 2023

OCA — Wed, 19 Jul 2023 06:55:26 GMT

←Older revision		Revision as of 06:55, 19 July 2023
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	<StructureSection load='5fkn' size='340' side='right'caption='[[5fkn]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='5fkn' size='340' side='right'caption='[[5fkn]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fkn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FKN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fkn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FKN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fkk\|5fkk]], [[5fkl\|5fkl]], [[5fkm\|5fkm]], [[5fko\|5fko]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkn OCA], [~~http~~://pdbe.org/5fkn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fkn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fkn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkn OCA], [https://pdbe.org/5fkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fkn RCSB], [https://www.ebi.ac.uk/pdbsum/5fkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TETR4_ECOLX TETR4_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:

	==See Also==		==See Also==
-	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]	+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hinrichs, W]]	+	[[Category: Hinrichs W]]
-	[[Category: Palm~~, G J~~]]	+	[[Category: Palm GJ]]
-	[[Category: Schneider, J]]	+	[[Category: Schneider J]]
-	[[Category: Werten, S]]	+	[[Category: Werten S]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Tetr]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 12:59, 10 May 2019

OCA — Fri, 10 May 2019 12:59:12 GMT

←Older revision		Revision as of 12:59, 10 May 2019
Line 1:		Line 1:

	==TetR(D) T103A mutant in complex with anhydrotetracycline and magnesium, P4(3)2(1)2==		==TetR(D) T103A mutant in complex with anhydrotetracycline and magnesium, P4(3)2(1)2==
-	<StructureSection load='5fkn' size='340' side='right' caption='[[5fkn]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='5fkn' size='340' side='right'caption='[[5fkn]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fkn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FKN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fkn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FKN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fkk\|5fkk]], [[5fkl\|5fkl]], [[5fkm\|5fkm]], [[5fko\|5fko]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fkk\|5fkk]], [[5fkl\|5fkl]], [[5fkm\|5fkm]], [[5fko\|5fko]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkn OCA], [http://pdbe.org/5fkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fkn RCSB], [http://www.ebi.ac.uk/pdbsum/5fkn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkn OCA], [http://pdbe.org/5fkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fkn RCSB], [http://www.ebi.ac.uk/pdbsum/5fkn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkn ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5fkn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fkn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein\|Tetracycline repressor protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]
	[[Category: Palm, G J]]		[[Category: Palm, G J]]

OCA at 17:29, 10 May 2016

OCA — Tue, 10 May 2016 17:29:05 GMT

←Older revision		Revision as of 17:29, 10 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5fkn is ~~ON HOLD until Paper~~ Publication	+	==TetR(D) T103A mutant in complex with anhydrotetracycline and magnesium, P4(3)2(1)2==
		+	<StructureSection load='5fkn' size='340' side='right' caption='[[5fkn]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fkn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FKN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fkk\|5fkk]], [[5fkl\|5fkl]], [[5fkm\|5fkm]], [[5fko\|5fko]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkn OCA], [http://pdbe.org/5fkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fkn RCSB], [http://www.ebi.ac.uk/pdbsum/5fkn PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Induction of the tetracycline repressor (TetR) results from antibiotic-dependent changes in the relative positioning of the DNA-binding domains within the promoter-associated repressor dimer, but the key determinants of this allosteric effect remain poorly characterised. Intriguingly, previous mutational analyses of the tetracycline-interacting site revealed a lack of correlation between residual affinity and induction propensity, suggesting that some of the residues in contact with the antibiotic primarily act in ligand recognition and retention, whereas others are required to transmit the allosteric signal. Here, we provide a structural basis for these observations via crystallographic analysis of the point mutants N82A, H100A, T103A and E147A in complex with the inducer 5a,6-anhydrotetracycline. In conjunction with the available functional data, the four structures demonstrate that a trigger-like movement of the region between helices alpha6 and alpha7 towards and into the binding site plays a decisive role in the intramolecular communication process. In sharp contrast, residues lining the binding cavity proper have little or no influence on the allosteric mechanism as such. This nearly complete physical separation of ligand recognition and allostery will have allowed diverging TetR-like repressors to bind novel effectors while the existing induction mechanism remained intact. Consequently, the modularity described here may have been a key factor in the evolutionary success of the widespread and highly diversified repressor class. DATABASE: Structural data are available in the Protein Data Bank under the accession numbers 5FKK, 5FKL, 5FKM, 5FKN and 5FKO.

-	~~Authors: Werten~~, S., Schneider, J., Palm~~, G.J.~~, Hinrichs, W.	+	Modular organisation of inducer recognition and allostery in the tetracycline repressor.,Werten S, Schneider J, Palm GJ, Hinrichs W FEBS J. 2016 Mar 30. doi: 10.1111/febs.13723. PMID:27028290<ref>PMID:27028290</ref>

-	~~Description: TetR(D) T103A mutant in complex with anhydrotetracycline and magnesium~~, ~~P4(3)2(1)2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5fkn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]
-	[[Category: Palm, G.J]]	+	[[Category: Palm, G J]]
	[[Category: Schneider, J]]		[[Category: Schneider, J]]
	[[Category: Werten, S]]		[[Category: Werten, S]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Repressor]]
		+	[[Category: Tetr]]
		+	[[Category: Transcription]]

OCA: Protected "5fkn" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 03:31:07 GMT

Protected "5fkn" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:31, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fkn is ON HOLD until Paper Publication Authors: Werten, S., Schneider, J., Palm, G.J., Hinrichs, W. Description: TetR(D) T103A mutant in complex w...

OCA — Tue, 01 Dec 2015 03:31:06 GMT

New page: '''Unreleased structure''' The entry 5fkn is ON HOLD until Paper Publication Authors: Werten, S., Schneider, J., Palm, G.J., Hinrichs, W. Description: TetR(D) T103A mutant in complex w...

New page

'''Unreleased structure'''

The entry 5fkn is ON HOLD until Paper Publication

Authors: Werten, S., Schneider, J., Palm, G.J., Hinrichs, W.

Description: TetR(D) T103A mutant in complex with anhydrotetracycline and magnesium, P4(3)2(1)2
[[Category: Unreleased Structures]]
[[Category: Hinrichs, W]]
[[Category: Palm, G.J]]
[[Category: Schneider, J]]
[[Category: Werten, S]]