5fmz - Revision history

OCA at 06:58, 19 July 2023

OCA — Wed, 19 Jul 2023 06:58:30 GMT

←Older revision		Revision as of 06:58, 19 July 2023
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	<StructureSection load='5fmz' size='340' side='right'caption='[[5fmz]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='5fmz' size='340' side='right'caption='[[5fmz]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fmz]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Influenza_b_virus_~~(b/~~memphis~~/13/03) Influenza b virus (b/~~memphis~~/13/03)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FMZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FMZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fmz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_B_virus_(B/Memphis/13/2003) Influenza B virus (B/Memphis/13/2003)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FMZ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5fml\|5fml]], [[5fmm\|5fmm]], [[5fmq\|5fmq]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA~~-~~directed_RNA_polymerase RNA-directed RNA polymerase]~~, ~~with EC number~~ [~~http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fmz OCA], [https://pdbe.org/5fmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fmz RCSB], [https://www.ebi.ac.uk/pdbsum/5fmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fmz ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fmz OCA], [~~http~~://pdbe.org/5fmz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fmz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fmz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fmz ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5V8Z9_9INFB Q5V8Z9_9INFB]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: RNA-directed RNA polymerase]]~~	+	[[Category: Cusack S]]
-	[[Category: Cusack, S]]	+	[[Category: Guilligay D]]
-	[[Category: Guilligay, D]]	+
-	~~[[Category: Heterotrimer]]~~	+
-	~~[[Category: Influenza b virus rna-dependent rna polymerase]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Viral rna]]~~	+
-	~~[[Category: Vrna 5' end~~]]	+

OCA at 07:26, 6 May 2020

OCA — Wed, 06 May 2020 07:26:50 GMT

←Older revision		Revision as of 07:26, 6 May 2020
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		+
	==Crystal structure of Influenza B polymerase with bound 5' vRNA==		==Crystal structure of Influenza B polymerase with bound 5' vRNA==
-	<StructureSection load='5fmz' size='340' side='right' caption='[[5fmz]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='5fmz' size='340' side='right'caption='[[5fmz]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fmz]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FMZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FMZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fmz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_b_virus_(b/memphis/13/03) Influenza b virus (b/memphis/13/03)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FMZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FMZ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fml\|5fml]], [[5fmm\|5fmm]], [[5fmq\|5fmq]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fml\|5fml]], [[5fmm\|5fmm]], [[5fmq\|5fmq]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fmz OCA], [http://pdbe.org/5fmz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fmz RCSB], [http://www.ebi.ac.uk/pdbsum/5fmz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fmz OCA], [http://pdbe.org/5fmz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fmz RCSB], [http://www.ebi.ac.uk/pdbsum/5fmz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fmz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5fmz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fmz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: RNA-directed RNA polymerase]]		[[Category: RNA-directed RNA polymerase]]
	[[Category: Cusack, S]]		[[Category: Cusack, S]]

OCA at 20:10, 13 January 2016

OCA — Wed, 13 Jan 2016 20:10:52 GMT

←Older revision		Revision as of 20:10, 13 January 2016
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-	'''~~Unreleased~~ structure'''	+	==Crystal structure of Influenza B polymerase with bound 5' vRNA==
		+	<StructureSection load='5fmz' size='340' side='right' caption='[[5fmz]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5fmz]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FMZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FMZ FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fml\|5fml]], [[5fmm\|5fmm]], [[5fmq\|5fmq]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fmz OCA], [http://pdbe.org/5fmz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fmz RCSB], [http://www.ebi.ac.uk/pdbsum/5fmz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Influenza virus polymerase transcribes or replicates the segmented RNA genome (vRNA) into respectively viral mRNA or full-length copies and initiates RNA synthesis by binding the conserved 3' and 5' vRNA ends (the promoter). In recent structures of promoter-bound polymerase, the cap-binding and endonuclease domains are configured for cap snatching, which generates capped transcription primers. Here, we present a FluB polymerase structure with a bound complementary cRNA 5' end that exhibits a major rearrangement of the subdomains within the C-terminal two-thirds of PB2 (PB2-C). Notably, the PB2 nuclear localization signal (NLS)-containing domain translocates approximately 90 A to bind to the endonuclease domain. FluA PB2-C alone and RNA-free FluC polymerase are similarly arranged. Biophysical and cap-dependent endonuclease assays show that in solution the polymerase explores different conformational distributions depending on which RNA is bound. The inherent flexibility of the polymerase allows it to adopt alternative conformations that are likely important during polymerase maturation into active progeny RNPs.

-	~~The entry 5fmz is ON HOLD until Paper Publication~~	+	Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains.,Thierry E, Guilligay D, Kosinski J, Bock T, Gaudon S, Round A, Pflug A, Hengrung N, El Omari K, Baudin F, Hart DJ, Beck M, Cusack S Mol Cell. 2016 Jan 7;61(1):125-37. doi: 10.1016/j.molcel.2015.11.016. Epub 2015, Dec 17. PMID:26711008<ref>PMID:26711008</ref>

-	~~Authors: Guilligay~~, D.~~, Cusack,~~ S.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of Influenza B polymerase with bound 5' vRNA~~	+	<div class="pdbe-citations 5fmz" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: RNA-directed RNA polymerase]]
	[[Category: Cusack, S]]		[[Category: Cusack, S]]
	[[Category: Guilligay, D]]		[[Category: Guilligay, D]]
		+	[[Category: Heterotrimer]]
		+	[[Category: Influenza b virus rna-dependent rna polymerase]]
		+	[[Category: Transcription]]
		+	[[Category: Viral rna]]
		+	[[Category: Vrna 5' end]]

OCA: Protected "5fmz" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 03:38:53 GMT

Protected "5fmz" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:38, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fmz is ON HOLD until Paper Publication Authors: Guilligay, D., Cusack, S. Description: Crystal structure of Influenza B polymerase with bound 5' v...

OCA — Tue, 01 Dec 2015 03:38:52 GMT

New page: '''Unreleased structure''' The entry 5fmz is ON HOLD until Paper Publication Authors: Guilligay, D., Cusack, S. Description: Crystal structure of Influenza B polymerase with bound 5' v...

New page

'''Unreleased structure'''

The entry 5fmz is ON HOLD until Paper Publication

Authors: Guilligay, D., Cusack, S.

Description: Crystal structure of Influenza B polymerase with bound 5' vRNA
[[Category: Unreleased Structures]]
[[Category: Cusack, S]]
[[Category: Guilligay, D]]