5fnz - Revision history

OCA at 07:00, 19 July 2023

OCA — Wed, 19 Jul 2023 07:00:39 GMT

←Older revision		Revision as of 07:00, 19 July 2023
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	<StructureSection load='5fnz' size='340' side='right'caption='[[5fnz]], [[Resolution\|resolution]] 2.52Å' scene=''>		<StructureSection load='5fnz' size='340' side='right'caption='[[5fnz]], [[Resolution\|resolution]] 2.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fnz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacterium_ammoniagenes"_cooke_and_keith_1927 "bacterium~~ ammoniagenes~~" cooke and keith 1927~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FNZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fnz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_ammoniagenes Corynebacterium ammoniagenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FNZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.52Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5fo0\|5fo0]]~~, ~~[[5fo1\|5fo1]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnz OCA], [~~http~~://pdbe.org/5fnz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fnz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fnz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnz OCA], [https://pdbe.org/5fnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fnz RCSB], [https://www.ebi.ac.uk/pdbsum/5fnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RIBF_CORAM RIBF_CORAM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium~~ ammoniagenes ~~cooke and keith 1927~~]]	+	[[Category: Corynebacterium ammoniagenes]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Herguedas, B]]	+	[[Category: Herguedas B]]
-	[[Category: Martinez-Julvez, M]]	+	[[Category: Martinez-Julvez M]]
-	[[Category: Milagros, M]]	+	[[Category: Milagros M]]
-	~~[[Category: Atp- binding]]~~	+
-	~~[[Category: Multifunctional enzyme]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Nucleotidyltransferase]]~~	+
-	~~[[Category: Riboflavin kinase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:27, 6 May 2020

OCA — Wed, 06 May 2020 07:27:48 GMT

←Older revision		Revision as of 07:27, 6 May 2020
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	==F206W mutant of FAD synthetase from Corynebacterium ammoniagenes==		==F206W mutant of FAD synthetase from Corynebacterium ammoniagenes==
-	<StructureSection load='5fnz' size='340' side='right' caption='[[5fnz]], [[Resolution\|resolution]] 2.52Å' scene=''>	+	<StructureSection load='5fnz' size='340' side='right'caption='[[5fnz]], [[Resolution\|resolution]] 2.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5fnz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5FNZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5fnz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_ammoniagenes"_cooke_and_keith_1927 "bacterium ammoniagenes" cooke and keith 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FNZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fo0\|5fo0]], [[5fo1\|5fo1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fo0\|5fo0]], [[5fo1\|5fo1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnz OCA], [http://pdbe.org/5fnz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fnz RCSB], [http://www.ebi.ac.uk/pdbsum/5fnz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnz OCA], [http://pdbe.org/5fnz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fnz RCSB], [http://www.ebi.ac.uk/pdbsum/5fnz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacterium ammoniagenes cooke and keith 1927]]
		+	[[Category: Large Structures]]
	[[Category: Herguedas, B]]		[[Category: Herguedas, B]]
	[[Category: Martinez-Julvez, M]]		[[Category: Martinez-Julvez, M]]

OCA at 06:37, 5 April 2017

OCA — Wed, 05 Apr 2017 06:37:05 GMT

←Older revision		Revision as of 06:37, 5 April 2017
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnz OCA], [http://pdbe.org/5fnz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fnz RCSB], [http://www.ebi.ac.uk/pdbsum/5fnz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnz OCA], [http://pdbe.org/5fnz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fnz RCSB], [http://www.ebi.ac.uk/pdbsum/5fnz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnz ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bifunctional FAD synthetases (FADSs) fold in two independent modules; The C-terminal riboflavin kinase (RFK) catalyzes the RFK activity, while the N-terminal FMN-adenylyltransferase (FMNAT) exhibits the FMNAT activity. The search for macromolecular interfaces in the Corynebacterium ammoniagenes FADS (CaFADS) crystal structure predicts a dimer of trimers organization. Within each trimer, a head-to-tail arrangement causes the RFK and FMNAT catalytic sites of the two neighboring protomers to approach, in agreement with active site residues of one module influencing the activity at the other. We analyze the relevance of the CaFADS head-to-tail macromolecular interfaces to stabilization of assemblies, catalysis and ligand binding. With this aim, we evaluate the effect of point mutations in loop L1c-FlapI, loop L6c, and helix alpha1c of the RFK module (positions K202, E203, F206, D298, V300, E301 and L304), regions at the macromolecular interface between two protomers within the trimer. Although none of the studied residues is critical in the formation and dissociation of assemblies, residues at L1c-FlapI and helix alpha1c particularly modulate quaternary architecture, as well as ligand binding and kinetic parameters involved with RFK and FMNAT activities. These data support the influence of transient oligomeric structures on substrate accommodation and catalysis at both CaFADS active sites.
		+
		+	The trimer interface in the quaternary structure of the bifunctional prokaryotic FAD synthetase from Corynebacterium ammoniagenes.,Serrano A, Sebastian M, Arilla-Luna S, Baquedano S, Herguedas B, Velazquez-Campoy A, Martinez-Julvez M, Medina M Sci Rep. 2017 Mar 24;7(1):404. doi: 10.1038/s41598-017-00402-6. PMID:28341845<ref>PMID:28341845</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5fnz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 18:31, 10 December 2016

OCA — Sat, 10 Dec 2016 18:31:53 GMT

←Older revision		Revision as of 18:31, 10 December 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5fnz is ~~ON HOLD until Paper Publication~~	+	==F206W mutant of FAD synthetase from Corynebacterium ammoniagenes==
-		+	<StructureSection load='5fnz' size='340' side='right' caption='[[5fnz]], [[Resolution\|resolution]] 2.52Å' scene=''>
-	~~Authors~~: ~~Martinez~~-~~Julvez~~, M., ~~Herguedas~~, B., ~~Milagros~~, M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5fnz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FNZ FirstGlance]. <br>
-	~~Description~~: ~~F206W mutant of FAD synthetase from Corynebacterium ammoniagenes~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fo0\|5fo0]], [[5fo1\|5fo1]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fnz OCA], [http://pdbe.org/5fnz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fnz RCSB], [http://www.ebi.ac.uk/pdbsum/5fnz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fnz ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Herguedas, B]]
		+	[[Category: Martinez-Julvez, M]]
	[[Category: Milagros, M]]		[[Category: Milagros, M]]
-	[[Category: ~~Martinez~~-~~Julvez, M~~]]	+	[[Category: Atp- binding]]
-	[[Category: ~~Herguedas, B~~]]	+	[[Category: Multifunctional enzyme]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Nucleotidyltransferase]]
		+	[[Category: Riboflavin kinase]]
		+	[[Category: Transferase]]

OCA at 15:22, 2 December 2015

OCA — Wed, 02 Dec 2015 15:22:47 GMT

←Older revision		Revision as of 15:22, 2 December 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5fnz is ON HOLD	+	The entry 5fnz is ON HOLD until Paper Publication

	Authors: Martinez-Julvez, M., Herguedas, B., Milagros, M.		Authors: Martinez-Julvez, M., Herguedas, B., Milagros, M.

OCA: Protected "5fnz" [edit=sysop:move=sysop]

OCA — Tue, 01 Dec 2015 03:43:03 GMT

Protected "5fnz" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:43, 1 December 2015

OCA: New page: '''Unreleased structure''' The entry 5fnz is ON HOLD Authors: Martinez-Julvez, M., Herguedas, B., Milagros, M. Description: F206W mutant of FAD synthetase from Corynebacterium ammoniag...

OCA — Tue, 01 Dec 2015 03:43:02 GMT

New page: '''Unreleased structure''' The entry 5fnz is ON HOLD Authors: Martinez-Julvez, M., Herguedas, B., Milagros, M. Description: F206W mutant of FAD synthetase from Corynebacterium ammoniag...

New page

'''Unreleased structure'''

The entry 5fnz is ON HOLD

Authors: Martinez-Julvez, M., Herguedas, B., Milagros, M.

Description: F206W mutant of FAD synthetase from Corynebacterium ammoniagenes
[[Category: Unreleased Structures]]
[[Category: Milagros, M]]
[[Category: Martinez-Julvez, M]]
[[Category: Herguedas, B]]