5g18 - Revision history

OCA at 13:35, 26 July 2023

OCA — Wed, 26 Jul 2023 13:35:01 GMT

←Older revision		Revision as of 13:35, 26 July 2023
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	<StructureSection load='5g18' size='340' side='right'caption='[[5g18]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='5g18' size='340' side='right'caption='[[5g18]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g18]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G18 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5G18 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g18]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G18 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZR:2-({[(1Z)-1-(2-AMINO-1,3-THIAZOL-4-YL)-2-OXO-2-{[(2S,3S)-1-OXO-3-(SULFOAMINO)BUTAN-2-YL]AMINO}ETHYLIDENE]AMINO}OXY)-2-METHYLPROPANOIC+ACID'>AZR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZR:2-({[(1Z)-1-(2-AMINO-1,3-THIAZOL-4-YL)-2-OXO-2-{[(2S,3S)-1-OXO-3-(SULFOAMINO)BUTAN-2-YL]AMINO}ETHYLIDENE]AMINO}OXY)-2-METHYLPROPANOIC+ACID'>AZR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5g18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g18 OCA], [~~http~~://pdbe.org/5g18 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5g18 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5g18 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5g18 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g18 OCA], [https://pdbe.org/5g18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g18 RCSB], [https://www.ebi.ac.uk/pdbsum/5g18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g18 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BLT1_ECOLX BLT1_ECOLX] Has strong cefotaxime-hydrolyzing activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Coates, L]]	+	[[Category: Coates L]]
-	[[Category: Cooper~~, J B~~]]	+	[[Category: Cooper JB]]
-	[[Category: Ginell~~, S L~~]]	+	[[Category: Ginell SL]]
-	[[Category: Parks~~, J M~~]]	+	[[Category: Parks JM]]
-	[[Category: Vandavasi~~, V G~~]]	+	[[Category: Vandavasi VG]]
-	[[Category: Weiss~~, K L]]~~	+	[[Category: Weiss KL]]
-	~~[[Category: Beta lactamase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Neutron]]~~	+
-	~~[[Category: Toho~~]]	+

OCA at 07:37, 23 October 2019

OCA — Wed, 23 Oct 2019 07:37:27 GMT

←Older revision		Revision as of 07:37, 23 October 2019
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	==Direct Observation of Active-site Protonation States in a Class A beta lactamase with a monobactam substrate==		==Direct Observation of Active-site Protonation States in a Class A beta lactamase with a monobactam substrate==
-	<StructureSection load='5g18' size='340' side='right' caption='[[5g18]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='5g18' size='340' side='right'caption='[[5g18]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g18]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G18 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G18 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZR:2-({[(1Z)-1-(2-AMINO-1,3-THIAZOL-4-YL)-2-OXO-2-{[(2S,3S)-1-OXO-3-(SULFOAMINO)BUTAN-2-YL]AMINO}ETHYLIDENE]AMINO}OXY)-2-METHYLPROPANOIC+ACID'>AZR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZR:2-({[(1Z)-1-(2-AMINO-1,3-THIAZOL-4-YL)-2-OXO-2-{[(2S,3S)-1-OXO-3-(SULFOAMINO)BUTAN-2-YL]AMINO}ETHYLIDENE]AMINO}OXY)-2-METHYLPROPANOIC+ACID'>AZR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
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	</div>		</div>
	<div class="pdbe-citations 5g18" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5g18" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Coates, L]]		[[Category: Coates, L]]
	[[Category: Cooper, J B]]		[[Category: Cooper, J B]]

OCA at 19:46, 9 December 2016

OCA — Fri, 09 Dec 2016 19:46:53 GMT

←Older revision		Revision as of 19:46, 9 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5g18 is ~~ON HOLD until Paper~~ Publication	+	==Direct Observation of Active-site Protonation States in a Class A beta lactamase with a monobactam substrate==
		+	<StructureSection load='5g18' size='340' side='right' caption='[[5g18]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5g18]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G18 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZR:2-({[(1Z)-1-(2-AMINO-1,3-THIAZOL-4-YL)-2-OXO-2-{[(2S,3S)-1-OXO-3-(SULFOAMINO)BUTAN-2-YL]AMINO}ETHYLIDENE]AMINO}OXY)-2-METHYLPROPANOIC+ACID'>AZR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g18 OCA], [http://pdbe.org/5g18 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g18 RCSB], [http://www.ebi.ac.uk/pdbsum/5g18 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g18 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The monobactam antibiotic aztreonam is used to treat cystic fibrosis patients with chronic pulmonary infections colonized by Pseudomonas aeruginosa strains expressing CTX-M extended-spectrum beta-lactamases. The protonation states of active site residues that are responsible for hydrolysis have been determined previously for the apo form of a CTX-M beta-lactamase, but not for a monobactam acyl-enzyme intermediate. Here we used neutron and high-resolution X-ray crystallography to probe the mechanism by which CTX-M extended-spectrum beta-lactamases hydrolyze monobactam antibiotics. In these first reported structures of a class A beta-lactamase in an acyl-enzyme complex with aztreonam we directly observed most of the hydrogen atoms (as deuterium) within the active site. Although Lys 234 is fully protonated in the acyl-intermediate, we find that Lys 73 is neutral. These findings are consistent with Lys 73 being able to serve as a general base during the acylation part of the catalytic mechanism, as previously proposed. SIGNIFICANCE: Since their initial discovery beta-lactams have constantly remained the most widely prescribed class of antibiotics due to their excellent safety, efficacy coupled with their high availability. The emergence and dissemination of bacterial resistance via beta-lactamase enzymes to this class of drug is now a serious problem in the clinic, often leaving few treatment options for infections resulting from multiple drug resistant superbugs. Using high resolution X-ray and neutron diffraction we have experimentally located key hydrogen atoms and been able to probe the exact mechanism by which extended-spectrum beta-lactamases are able to hydrolyze monobactam antibiotics.

-	~~Authors:~~ Vandavasi, ~~V.G.~~, Weiss~~, K.L.~~, Parks~~, J.M.~~, Cooper~~, J.B.~~, Ginell~~, S.L.~~, Coates, L.	+	Active Site Protonation States in an Acyl-enzyme Intermediate of a Class A beta-lactamase with a Monobactam Substrate.,Vandavasi VG, Langan PS, Weiss KL, Parks JM, Cooper JB, Ginell SL, Coates L Antimicrob Agents Chemother. 2016 Oct 24. pii: AAC.01636-16. PMID:27795378<ref>PMID:27795378</ref>

-	~~Description: Direct Observation~~ of ~~Active~~-~~site Protonation States in a Class A beta lactamase with a monobactam substrate~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5g18" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-lactamase]]
	[[Category: Coates, L]]		[[Category: Coates, L]]
-	[[Category: ~~Parks~~, J.M]]	+	[[Category: Cooper, J B]]
-	[[Category: ~~Cooper~~, J.B]]	+	[[Category: Ginell, S L]]
-	[[Category: Vandavasi, V.G]]	+	[[Category: Parks, J M]]
-	[[Category: Weiss, K.L]]	+	[[Category: Vandavasi, V G]]
-	[[Category: ~~Ginell, S.L~~]]	+	[[Category: Weiss, K L]]
		+	[[Category: Beta lactamase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Neutron]]
		+	[[Category: Toho]]

OCA at 12:09, 2 November 2016

OCA — Wed, 02 Nov 2016 12:09:42 GMT

←Older revision		Revision as of 12:09, 2 November 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5g18 is ON HOLD	+	The entry 5g18 is ON HOLD until Paper Publication

	Authors: Vandavasi, V.G., Weiss, K.L., Parks, J.M., Cooper, J.B., Ginell, S.L., Coates, L.		Authors: Vandavasi, V.G., Weiss, K.L., Parks, J.M., Cooper, J.B., Ginell, S.L., Coates, L.

OCA: Protected "5g18" [edit=sysop:move=sysop]

OCA — Tue, 10 May 2016 23:20:25 GMT

Protected "5g18" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:20, 10 May 2016

OCA: New page: '''Unreleased structure''' The entry 5g18 is ON HOLD Authors: Vandavasi, V.G., Weiss, K.L., Parks, J.M., Cooper, J.B., Ginell, S.L., Coates, L. Description: Direct Observation of Activ...

OCA — Tue, 10 May 2016 23:20:24 GMT

New page: '''Unreleased structure''' The entry 5g18 is ON HOLD Authors: Vandavasi, V.G., Weiss, K.L., Parks, J.M., Cooper, J.B., Ginell, S.L., Coates, L. Description: Direct Observation of Activ...

New page

'''Unreleased structure'''

The entry 5g18 is ON HOLD

Authors: Vandavasi, V.G., Weiss, K.L., Parks, J.M., Cooper, J.B., Ginell, S.L., Coates, L.

Description: Direct Observation of Active-site Protonation States in a Class A beta lactamase with a monobactam substrate
[[Category: Unreleased Structures]]
[[Category: Coates, L]]
[[Category: Parks, J.M]]
[[Category: Cooper, J.B]]
[[Category: Vandavasi, V.G]]
[[Category: Weiss, K.L]]
[[Category: Ginell, S.L]]