5g1n - Revision history

OCA at 13:35, 26 July 2023

OCA — Wed, 26 Jul 2023 13:35:17 GMT

←Older revision		Revision as of 13:35, 26 July 2023
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	<StructureSection load='5g1n' size='340' side='right'caption='[[5g1n]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5g1n' size='340' side='right'caption='[[5g1n]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g1n]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G1N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5G1N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g1n]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G1N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G1N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PAL:N-(PHOSPHONACETYL)-L-ASPARTIC+ACID'>PAL</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g1o\|5g1o]], [[5g1p\|5g1p]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PAL:N-(PHOSPHONACETYL)-L-ASPARTIC+ACID'>PAL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5g1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g1n OCA], [~~http~~://pdbe.org/5g1n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5g1n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5g1n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5g1n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g1n OCA], [https://pdbe.org/5g1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g1n RCSB], [https://www.ebi.ac.uk/pdbsum/5g1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g1n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PYR1_HUMAN PYR1_HUMAN]] This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).	+	[https://www.uniprot.org/uniprot/PYR1_HUMAN PYR1_HUMAN] This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5g1n" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5g1n" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[CAD protein 3D structures\|CAD protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Grande-Garcia, A]]	+	[[Category: Grande-Garcia A]]
-	[[Category: Moreno-Morcillo~~, M D~~]]	+	[[Category: Moreno-Morcillo MD]]
-	[[Category: Ramon-Maiques, S]]	+	[[Category: Ramon-Maiques S]]
-	[[Category: Ruiz-Ramos, A]]	+	[[Category: Ruiz-Ramos A]]
-	~~[[Category: Cad]]~~	+
-	~~[[Category: Carbamoyl phosphate synthetase]]~~	+
-	~~[[Category: Cooperativity]]~~	+
-	~~[[Category: De novo pyrimidine synthesis]]~~	+
-	~~[[Category: Dihydroorotase]]~~	+
-	~~[[Category: Transcarbamoylase]]~~	+
-	~~[[Category: Transcarbamylase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:34, 13 May 2020

OCA — Wed, 13 May 2020 06:34:54 GMT

←Older revision		Revision as of 06:34, 13 May 2020
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	==Aspartate transcarbamoylase domain of human CAD bound to PALA==		==Aspartate transcarbamoylase domain of human CAD bound to PALA==
-	<StructureSection load='5g1n' size='340' side='right' caption='[[5g1n]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5g1n' size='340' side='right'caption='[[5g1n]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g1n]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G1N OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5G1N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g1n]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G1N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5G1N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PAL:N-(PHOSPHONACETYL)-L-ASPARTIC+ACID'>PAL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PAL:N-(PHOSPHONACETYL)-L-ASPARTIC+ACID'>PAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g1o\|5g1o]], [[5g1p\|5g1p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g1o\|5g1o]], [[5g1p\|5g1p]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5g1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g1n OCA], [http://pdbe.org/5g1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g1n RCSB], [http://www.ebi.ac.uk/pdbsum/5g1n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g1n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5g1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g1n OCA], [http://pdbe.org/5g1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g1n RCSB], [http://www.ebi.ac.uk/pdbsum/5g1n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g1n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Grande-Garcia, A]]		[[Category: Grande-Garcia, A]]
	[[Category: Moreno-Morcillo, M D]]		[[Category: Moreno-Morcillo, M D]]

OCA at 02:26, 13 July 2016

OCA — Wed, 13 Jul 2016 02:26:30 GMT

←Older revision		Revision as of 02:26, 13 July 2016
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PAL:N-(PHOSPHONACETYL)-L-ASPARTIC+ACID'>PAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PAL:N-(PHOSPHONACETYL)-L-ASPARTIC+ACID'>PAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g1o\|5g1o]], [[5g1p\|5g1p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g1o\|5g1o]], [[5g1p\|5g1p]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g1n OCA], [http://pdbe.org/5g1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g1n RCSB], [http://www.ebi.ac.uk/pdbsum/5g1n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g1n OCA], [http://pdbe.org/5g1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g1n RCSB], [http://www.ebi.ac.uk/pdbsum/5g1n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g1n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 23:17, 23 June 2016

OCA — Thu, 23 Jun 2016 23:17:57 GMT

←Older revision		Revision as of 23:17, 23 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5g1n is ~~ON HOLD until Paper~~ Publication	+	==Aspartate transcarbamoylase domain of human CAD bound to PALA==
		+	<StructureSection load='5g1n' size='340' side='right' caption='[[5g1n]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5g1n]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G1N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PAL:N-(PHOSPHONACETYL)-L-ASPARTIC+ACID'>PAL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g1o\|5g1o]], [[5g1p\|5g1p]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g1n OCA], [http://pdbe.org/5g1n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g1n RCSB], [http://www.ebi.ac.uk/pdbsum/5g1n PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PYR1_HUMAN PYR1_HUMAN]] This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CAD, the multienzymatic protein that initiates and controls de novo synthesis of pyrimidines in animals, associates through its aspartate transcarbamoylase (ATCase) domain into particles of 1.5 MDa. Despite numerous structures of prokaryotic ATCases, we lack structural information on the ATCase domain of CAD. Here, we report the structure and functional characterization of human ATCase, confirming the overall similarity with bacterial homologs. Unexpectedly, human ATCase exhibits cooperativity effects that reduce the affinity for the anti-tumoral drug PALA. Combining structural, mutagenic, and biochemical analysis, we identified key elements for the necessary regulation and transmission of conformational changes leading to cooperativity between subunits. Mutation of one of these elements, R2024, was recently found to cause the first non-lethal CAD deficit. We reproduced this mutation in human ATCase and measured its effect, demonstrating that this arginine is part of a molecular switch that regulates the equilibrium between low- and high-affinity states for the ligands.

-	~~Authors:~~ Ruiz-Ramos, A., Grande-Garcia, A., Moreno-Morcillo, M~~.D.~~, Ramon-Maiques, S.	+	Structure and Functional Characterization of Human Aspartate Transcarbamoylase, the Target of the Anti-tumoral Drug PALA.,Ruiz-Ramos A, Velazquez-Campoy A, Grande-Garcia A, Moreno-Morcillo M, Ramon-Maiques S Structure. 2016 May 31. pii: S0969-2126(16)30079-X. doi:, 10.1016/j.str.2016.05.001. PMID:27265852<ref>PMID:27265852</ref>

-	~~Description: Aspartate transcarbamoylase domain~~ of ~~human CAD bound to PALA~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category: Moreno~~-~~Morcillo, M.D]]~~	+	<div class="pdbe-citations 5g1n" style="background-color:#fffaf0;"></div>
-	~~[[Category: Ruiz~~-~~Ramos, A]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Grande-Garcia, A]]		[[Category: Grande-Garcia, A]]
		+	[[Category: Moreno-Morcillo, M D]]
	[[Category: Ramon-Maiques, S]]		[[Category: Ramon-Maiques, S]]
		+	[[Category: Ruiz-Ramos, A]]
		+	[[Category: Cad]]
		+	[[Category: Carbamoyl phosphate synthetase]]
		+	[[Category: Cooperativity]]
		+	[[Category: De novo pyrimidine synthesis]]
		+	[[Category: Dihydroorotase]]
		+	[[Category: Transcarbamoylase]]
		+	[[Category: Transcarbamylase]]
		+	[[Category: Transferase]]

OCA: Protected "5g1n" [edit=sysop:move=sysop]

OCA — Tue, 10 May 2016 23:21:40 GMT

Protected "5g1n" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:21, 10 May 2016

OCA: New page: '''Unreleased structure''' The entry 5g1n is ON HOLD until Paper Publication Authors: Ruiz-Ramos, A., Grande-Garcia, A., Moreno-Morcillo, M.D., Ramon-Maiques, S. Description: Aspartate...

OCA — Tue, 10 May 2016 23:21:38 GMT

New page: '''Unreleased structure''' The entry 5g1n is ON HOLD until Paper Publication Authors: Ruiz-Ramos, A., Grande-Garcia, A., Moreno-Morcillo, M.D., Ramon-Maiques, S. Description: Aspartate...

New page

'''Unreleased structure'''

The entry 5g1n is ON HOLD until Paper Publication

Authors: Ruiz-Ramos, A., Grande-Garcia, A., Moreno-Morcillo, M.D., Ramon-Maiques, S.

Description: Aspartate transcarbamoylase domain of human CAD bound to PALA
[[Category: Unreleased Structures]]
[[Category: Moreno-Morcillo, M.D]]
[[Category: Ruiz-Ramos, A]]
[[Category: Grande-Garcia, A]]
[[Category: Ramon-Maiques, S]]