5g27 - Revision history

OCA at 13:35, 26 July 2023

OCA — Wed, 26 Jul 2023 13:35:51 GMT

←Older revision		Revision as of 13:35, 26 July 2023
Line 3:		Line 3:
	<StructureSection load='5g27' size='340' side='right'caption='[[5g27]], [[Resolution\|resolution]] 1.61Å' scene=''>		<StructureSection load='5g27' size='340' side='right'caption='[[5g27]], [[Resolution\|resolution]] 1.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g27]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bpt4 Bpt4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G27 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5G27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g27]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G27 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.61Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5g27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g27 OCA], [~~http~~://pdbe.org/5g27 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5g27 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5g27 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5g27 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g27 OCA], [https://pdbe.org/5g27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g27 RCSB], [https://www.ebi.ac.uk/pdbsum/5g27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g27 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>	+	[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5g27" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5g27" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysin 3D structures\|Lysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpt4~~]]	+	[[Category: Escherichia virus T4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Consentius P]]
-	[[Category: Consentius, P]]	+	[[Category: Gohlke U]]
-	[[Category: Gohlke, U]]	+	[[Category: Heinemann U]]
-	[[Category: Heinemann, U]]	+	[[Category: Kaupp M]]
-	[[Category: Kaupp, M]]	+	[[Category: Loll B]]
-	[[Category: Loll, B]]	+	[[Category: Mueller R]]
-	[[Category: Mueller, R]]	+	[[Category: Risse T]]
-	[[Category: Risse, T]]	+
-	~~[[Category: Electron]]~~	+
-	~~[[Category: Epr]]~~	+
-	~~[[Category: Esr]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Labelling]]~~	+
-	~~[[Category: Paramagnetic]]~~	+
-	~~[[Category: Resonance]]~~	+
-	~~[[Category: Spin]]~~	+
-	~~[[Category: T4 lysozyme~~]]	+

OCA at 06:35, 13 May 2020

OCA — Wed, 13 May 2020 06:35:56 GMT

←Older revision		Revision as of 06:35, 13 May 2020
Line 1:		Line 1:

	==Structure of Spin-labelled T4 lysozyme mutant L118C-R1 at Room Temperature==		==Structure of Spin-labelled T4 lysozyme mutant L118C-R1 at Room Temperature==
-	<StructureSection load='5g27' size='340' side='right' caption='[[5g27]], [[Resolution\|resolution]] 1.61Å' scene=''>	+	<StructureSection load='5g27' size='340' side='right'caption='[[5g27]], [[Resolution\|resolution]] 1.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g27]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G27 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5G27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g27]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G27 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5G27 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5g27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g27 OCA], [http://pdbe.org/5g27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g27 RCSB], [http://www.ebi.ac.uk/pdbsum/5g27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g27 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5g27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g27 OCA], [http://pdbe.org/5g27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g27 RCSB], [http://www.ebi.ac.uk/pdbsum/5g27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g27 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bpt4]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Consentius, P]]		[[Category: Consentius, P]]

OCA at 10:51, 10 December 2016

OCA — Sat, 10 Dec 2016 10:51:32 GMT

←Older revision		Revision as of 10:51, 10 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5g27 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of Spin-labelled T4 lysozyme mutant L118C-R1 at Room Temperature==
		+	<StructureSection load='5g27' size='340' side='right' caption='[[5g27]], [[Resolution\|resolution]] 1.61Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5g27]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G27 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g27 OCA], [http://pdbe.org/5g27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g27 RCSB], [http://www.ebi.ac.uk/pdbsum/5g27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g27 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins are dynamic molecules that can transiently adopt different conformational states. As the function of the system often depends critically on its conformational state a rigorous understanding of the correlation between structure, energetics and dynamics of the different accessible states is crucial. The biophysical characterization of such processes is, however, challenging as the excited states are often only marginally populated. We show that a combination of X-ray crystallography performed at 100 K as well as at room temperature and EPR spectroscopy on a spin-labeled single crystal allows to correlate the structures of the ground state and a thermally excited state with their thermodynamics using the variant 118R1 of T4 lysozyme as an example. In addition, it is shown that the surrounding solvent can significantly alter the energetic as well as the entropic contribution to the Gibbs free energy without major impact on the structure of both states.

-	~~Authors: Gohlke, U~~., Consentius, P., Loll, B., ~~Mueller~~, R., Kaupp, M~~., Heinemann, U.~~, Risse, T.	+	Tracking Transient Conformational States of T4 Lysozyme at Room Temperature Combining X-ray Crystallography and Site-Directed Spin Labeling.,Consentius P, Gohlke U, Loll B, Alings C, Muller R, Heinemann U, Kaupp M, Wahl M, Risse T J Am Chem Soc. 2016 Oct 5;138(39):12868-12875. Epub 2016 Sep 27. PMID:27673570<ref>PMID:27673570</ref>

-	~~Description: Structure~~ of ~~Spin~~-~~labelled T4 lysozyme mutant L118C~~-~~R1 at Room Temperature~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5g27" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lysozyme]]
	[[Category: Consentius, P]]		[[Category: Consentius, P]]
-	[[Category: ~~Mueller~~, ~~R]]~~	+	[[Category: Gohlke, U]]
-	~~[[Category: Loll, B~~]]	+
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]
	[[Category: Kaupp, M]]		[[Category: Kaupp, M]]
-	[[Category: ~~Gohlke~~, U]]	+	[[Category: Loll, B]]
		+	[[Category: Mueller, R]]
	[[Category: Risse, T]]		[[Category: Risse, T]]
		+	[[Category: Electron]]
		+	[[Category: Epr]]
		+	[[Category: Esr]]
		+	[[Category: Hydrolase]]
		+	[[Category: Labelling]]
		+	[[Category: Paramagnetic]]
		+	[[Category: Resonance]]
		+	[[Category: Spin]]
		+	[[Category: T4 lysozyme]]

OCA: Protected "5g27" [edit=sysop:move=sysop]

OCA — Tue, 10 May 2016 23:23:26 GMT

Protected "5g27" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:23, 10 May 2016

OCA: New page: '''Unreleased structure''' The entry 5g27 is ON HOLD until Paper Publication Authors: Gohlke, U., Consentius, P., Loll, B., Mueller, R., Kaupp, M., Heinemann, U., Risse, T. Description...

OCA — Tue, 10 May 2016 23:23:24 GMT

New page: '''Unreleased structure''' The entry 5g27 is ON HOLD until Paper Publication Authors: Gohlke, U., Consentius, P., Loll, B., Mueller, R., Kaupp, M., Heinemann, U., Risse, T. Description...

New page

'''Unreleased structure'''

The entry 5g27 is ON HOLD until Paper Publication

Authors: Gohlke, U., Consentius, P., Loll, B., Mueller, R., Kaupp, M., Heinemann, U., Risse, T.

Description: Structure of Spin-labelled T4 lysozyme mutant L118C-R1 at Room Temperature
[[Category: Unreleased Structures]]
[[Category: Consentius, P]]
[[Category: Mueller, R]]
[[Category: Loll, B]]
[[Category: Heinemann, U]]
[[Category: Kaupp, M]]
[[Category: Gohlke, U]]
[[Category: Risse, T]]