5g31 - Revision history

OCA at 13:37, 26 July 2023

OCA — Wed, 26 Jul 2023 13:37:01 GMT

←Older revision		Revision as of 13:37, 26 July 2023
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	<StructureSection load='5g31' size='340' side='right'caption='[[5g31]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5g31' size='340' side='right'caption='[[5g31]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g31]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Litopenaeus_vannamei Litopenaeus~~ vannamei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G31 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5G31 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g31]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penaeus_vannamei Penaeus vannamei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G31 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5g2z\|5g2z]], [[5g30\|5g30]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [https://pdbe.org/5g31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [https://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [~~http~~://pdbe.org/5g31 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B1PWB9_PENVA B1PWB9_PENVA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Litopenaeus~~ vannamei]]	+	[[Category: Penaeus vannamei]]
-	~~[[Category: Thioredoxin-disulfide reductase~~]]	+	[[Category: Campos-Acevedo AA]]
-	[[Category: Campos-Acevedo~~, A A~~]]	+	[[Category: Rudino-Pinera E]]
-	[[Category: Rudino-Pinera, E]]	+
-	~~[[Category: Disulfide bond]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Shrimp]]~~	+
-	~~[[Category: Thioredoxin~~]]	+

OCA at 06:37, 13 May 2020

OCA — Wed, 13 May 2020 06:37:11 GMT

←Older revision		Revision as of 06:37, 13 May 2020
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	==Crystallographic structure of mutant C73S of thioredoxin from Litopenaeus vannamei==		==Crystallographic structure of mutant C73S of thioredoxin from Litopenaeus vannamei==
-	<StructureSection load='5g31' size='340' side='right' caption='[[5g31]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5g31' size='340' side='right'caption='[[5g31]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g31]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G31 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5G31 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g31]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Litopenaeus_vannamei Litopenaeus vannamei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G31 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5G31 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g2z\|5g2z]], [[5g30\|5g30]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g2z\|5g2z]], [[5g30\|5g30]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [http://pdbe.org/5g31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [http://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [http://pdbe.org/5g31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [http://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Litopenaeus vannamei]]
	[[Category: Thioredoxin-disulfide reductase]]		[[Category: Thioredoxin-disulfide reductase]]
	[[Category: Campos-Acevedo, A A]]		[[Category: Campos-Acevedo, A A]]
	[[Category: Rudino-Pinera, E]]		[[Category: Rudino-Pinera, E]]
	[[Category: Disulfide bond]]		[[Category: Disulfide bond]]
-	[[Category: Litopenaeus vannamei]]
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Shrimp]]		[[Category: Shrimp]]
	[[Category: Thioredoxin]]		[[Category: Thioredoxin]]

OCA at 06:18, 19 April 2017

OCA — Wed, 19 Apr 2017 06:18:03 GMT

←Older revision		Revision as of 06:18, 19 April 2017
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [http://pdbe.org/5g31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [http://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [http://pdbe.org/5g31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [http://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The quaternary structure of the redox protein thioredoxin (Trx) has been debated. For bacterial Trx, there is no question regarding its monomeric state. In humans and other eukaryotes, the presence of a cysteine residue at the crystallographic symmetry axis points to the relevance of dimer formation in solution and in vivo. Crystallographic data for shrimp thioredoxin (LvTrx) obtained under different redox conditions reveal a dimeric arrangement mediated by a disulfide bond through residue Cys73 and other hydrophobic interactions located in the crystallographic interface, as reported for human Trx. Through the analysis of five mutants located at the crystallographic interface, this study provides structural and biochemical evidence for the existence in solution of monomeric and dimeric populations of wild-type LvTrx and five mutants. Based on the results of biochemical assays, SAXS studies and the crystallographic structures of three of the studied mutants (Cys73Ser, Asp60Ser and Trp31Ala), it is clear that the Cys73 residue is essential for dimerization. However, its mutation to Ser produces an enzyme which has similar redox activity in vitro to the wild type. A putative regulatory function of dimerization is proposed based on structural analysis. Nonetheless, the biological role of LvTrx dimerization needs to be experimentally unveiled. Additionally, the findings of this work reopen the discussion regarding the existence of similar behaviour in human thioredoxin, which shares a Cys at position 73 with LvTrx, a structural feature that is also present in some Trxs from vertebrates and crustaceans.
		+
		+	Is dimerization a common feature in thioredoxins? The case of thioredoxin from Litopenaeus vannamei.,Campos-Acevedo AA, Sotelo-Mundo RR, Perez J, Rudino-Pinera E Acta Crystallogr D Struct Biol. 2017 Apr 1;73(Pt 4):326-339. doi:, 10.1107/S2059798317002066. Epub 2017 Mar 31. PMID:28375144<ref>PMID:28375144</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5g31" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 21:14, 15 March 2017

OCA — Wed, 15 Mar 2017 21:14:35 GMT

←Older revision		Revision as of 21:14, 15 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5g31 is ~~ON HOLD until Paper Publication~~	+	==Crystallographic structure of mutant C73S of thioredoxin from Litopenaeus vannamei==
-		+	<StructureSection load='5g31' size='340' side='right' caption='[[5g31]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~: ~~Campos~~-~~Acevedo~~, A.A., ~~Rudino~~-~~Pinera~~, E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5g31]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G31 FirstGlance]. <br>
-	~~Description: Crystallographic structure of mutant C73S of thioredoxin from Litopenaeus vannamei~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g2z\|5g2z]], [[5g30\|5g30]]</td></tr>
-	[[Category: Campos-Acevedo, A.A]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [http://pdbe.org/5g31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [http://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Thioredoxin-disulfide reductase]]
		+	[[Category: Campos-Acevedo, A A]]
	[[Category: Rudino-Pinera, E]]		[[Category: Rudino-Pinera, E]]
		+	[[Category: Disulfide bond]]
		+	[[Category: Litopenaeus vannamei]]
		+	[[Category: Mutant]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Shrimp]]
		+	[[Category: Thioredoxin]]

OCA: Protected "5g31" [edit=sysop:move=sysop]

OCA — Tue, 10 May 2016 23:26:41 GMT

Protected "5g31" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:26, 10 May 2016

OCA: New page: '''Unreleased structure''' The entry 5g31 is ON HOLD until Paper Publication Authors: Campos-Acevedo, A.A., Rudino-Pinera, E. Description: Crystallographic structure of mutant C73S of ...

OCA — Tue, 10 May 2016 23:26:39 GMT

New page: '''Unreleased structure''' The entry 5g31 is ON HOLD until Paper Publication Authors: Campos-Acevedo, A.A., Rudino-Pinera, E. Description: Crystallographic structure of mutant C73S of ...

New page

'''Unreleased structure'''

The entry 5g31 is ON HOLD until Paper Publication

Authors: Campos-Acevedo, A.A., Rudino-Pinera, E.

Description: Crystallographic structure of mutant C73S of thioredoxin from Litopenaeus vannamei
[[Category: Unreleased Structures]]
[[Category: Campos-Acevedo, A.A]]
[[Category: Rudino-Pinera, E]]