5g3x - Revision history

OCA at 13:38, 26 July 2023

OCA — Wed, 26 Jul 2023 13:38:00 GMT

←Older revision		Revision as of 13:38, 26 July 2023
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	<StructureSection load='5g3x' size='340' side='right'caption='[[5g3x]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='5g3x' size='340' side='right'caption='[[5g3x]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g3x]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cpgv Cpgv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G3X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5G3X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g3x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cydia_pomonella_granulovirus Cydia pomonella granulovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G3X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5g3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g3x OCA], [~~http~~://pdbe.org/5g3x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5g3x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5g3x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5g3x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.66Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g3x OCA], [https://pdbe.org/5g3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g3x RCSB], [https://www.ebi.ac.uk/pdbsum/5g3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g3x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GRAN_GVCPM GRAN_GVCPM]] Component of the virus occlusion bodies, which are large proteinaceous structures, that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.	+	[https://www.uniprot.org/uniprot/GRAN_GVCPM GRAN_GVCPM] Component of the virus occlusion bodies, which are large proteinaceous structures, that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5g3x" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5g3x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Polyhedrin\|Polyhedrin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cpgv~~]]	+	[[Category: Cydia pomonella granulovirus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bunker~~, R D~~]]	+	[[Category: Bunker RD]]
-	[[Category: Chiu, E]]	+	[[Category: Chiu E]]
-	[[Category: Metcalf, P]]	+	[[Category: Metcalf P]]
-	~~[[Category: In vivo crystallization]]~~	+
-	~~[[Category: Nano crystallography]]~~	+
-	~~[[Category: Native crystal]]~~	+
-	~~[[Category: Viral protein]]~~	+
-	~~[[Category: Virus~~]]	+

OCA at 07:37, 23 October 2019

OCA — Wed, 23 Oct 2019 07:37:45 GMT

←Older revision		Revision as of 07:37, 23 October 2019
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	==Structure of recombinant granulovirus polyhedrin==		==Structure of recombinant granulovirus polyhedrin==
-	<StructureSection load='5g3x' size='340' side='right' caption='[[5g3x]], [[Resolution\|resolution]] 1.66Å' scene=''>	+	<StructureSection load='5g3x' size='340' side='right'caption='[[5g3x]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g3x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G3X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g3x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cpgv Cpgv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G3X FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g3x OCA], [http://pdbe.org/5g3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g3x RCSB], [http://www.ebi.ac.uk/pdbsum/5g3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g3x ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g3x OCA], [http://pdbe.org/5g3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g3x RCSB], [http://www.ebi.ac.uk/pdbsum/5g3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g3x ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cpgv]]
		+	[[Category: Large Structures]]
	[[Category: Bunker, R D]]		[[Category: Bunker, R D]]
	[[Category: Chiu, E]]		[[Category: Chiu, E]]

OCA at 07:41, 9 March 2017

OCA — Thu, 09 Mar 2017 07:41:19 GMT

←Older revision		Revision as of 07:41, 9 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5g3x is ~~ON HOLD~~ until ~~Paper~~ Publication	+	==Structure of recombinant granulovirus polyhedrin==
		+	<StructureSection load='5g3x' size='340' side='right' caption='[[5g3x]], [[Resolution\|resolution]] 1.66Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5g3x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G3X FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g3x OCA], [http://pdbe.org/5g3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g3x RCSB], [http://www.ebi.ac.uk/pdbsum/5g3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g3x ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GRAN_GVCPM GRAN_GVCPM]] Component of the virus occlusion bodies, which are large proteinaceous structures, that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To understand how molecules function in biological systems, new methods are required to obtain atomic resolution structures from biological material under physiological conditions. Intense femtosecond-duration pulses from X-ray free-electron lasers (XFELs) can outrun most damage processes, vastly increasing the tolerable dose before the specimen is destroyed. This in turn allows structure determination from crystals much smaller and more radiation sensitive than previously considered possible, allowing data collection from room temperature structures and avoiding structural changes due to cooling. Regardless, high-resolution structures obtained from XFEL data mostly use crystals far larger than 1 mum3 in volume, whereas the X-ray beam is often attenuated to protect the detector from damage caused by intense Bragg spots. Here, we describe the 2 A resolution structure of native nanocrystalline granulovirus occlusion bodies (OBs) that are less than 0.016 mum3 in volume using the full power of the Linac Coherent Light Source (LCLS) and a dose up to 1.3 GGy per crystal. The crystalline shell of granulovirus OBs consists, on average, of about 9,000 unit cells, representing the smallest protein crystals to yield a high-resolution structure by X-ray crystallography to date. The XFEL structure shows little to no evidence of radiation damage and is more complete than a model determined using synchrotron data from recombinantly produced, much larger, cryocooled granulovirus granulin microcrystals. Our measurements suggest that it should be possible, under ideal experimental conditions, to obtain data from protein crystals with only 100 unit cells in volume using currently available XFELs and suggest that single-molecule imaging of individual biomolecules could almost be within reach.

-	~~Authors: CHIU~~, E., ~~BUNKER~~, R.D., ~~METCALF~~, P.	+	Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser.,Gati C, Oberthuer D, Yefanov O, Bunker RD, Stellato F, Chiu E, Yeh SM, Aquila A, Basu S, Bean R, Beyerlein KR, Botha S, Boutet S, DePonte DP, Doak RB, Fromme R, Galli L, Grotjohann I, James DR, Kupitz C, Lomb L, Messerschmidt M, Nass K, Rendek K, Shoeman RL, Wang D, Weierstall U, White TA, Williams GJ, Zatsepin NA, Fromme P, Spence JC, Goldie KN, Jehle JA, Metcalf P, Barty A, Chapman HN Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2247-2252. doi:, 10.1073/pnas.1609243114. Epub 2017 Feb 15. PMID:28202732<ref>PMID:28202732</ref>

-	~~Description: Structure~~ of ~~recombinant granulovirus polyhedrin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5g3x" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bunker, R D]]
	[[Category: Chiu, E]]		[[Category: Chiu, E]]
-	[[Category: Bunker, R.D]]
	[[Category: Metcalf, P]]		[[Category: Metcalf, P]]
		+	[[Category: In vivo crystallization]]
		+	[[Category: Nano crystallography]]
		+	[[Category: Native crystal]]
		+	[[Category: Viral protein]]
		+	[[Category: Virus]]

OCA at 11:38, 1 June 2016

OCA — Wed, 01 Jun 2016 11:38:09 GMT

←Older revision		Revision as of 11:38, 1 June 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5g3x is ON HOLD	+	The entry 5g3x is ON HOLD until Paper Publication

	Authors: CHIU, E., BUNKER, R.D., METCALF, P.		Authors: CHIU, E., BUNKER, R.D., METCALF, P.

OCA: Protected "5g3x" [edit=sysop:move=sysop]

OCA — Sun, 15 May 2016 13:27:26 GMT

Protected "5g3x" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:27, 15 May 2016

OCA: New page: '''Unreleased structure''' The entry 5g3x is ON HOLD Authors: CHIU, E., BUNKER, R.D., METCALF, P. Description: Structure of recombinant granulovirus polyhedrin [[Category: Unreleased S...

OCA — Sun, 15 May 2016 13:27:25 GMT

New page: '''Unreleased structure''' The entry 5g3x is ON HOLD Authors: CHIU, E., BUNKER, R.D., METCALF, P. Description: Structure of recombinant granulovirus polyhedrin [[Category: Unreleased S...

New page

'''Unreleased structure'''

The entry 5g3x is ON HOLD

Authors: CHIU, E., BUNKER, R.D., METCALF, P.

Description: Structure of recombinant granulovirus polyhedrin
[[Category: Unreleased Structures]]
[[Category: Chiu, E]]
[[Category: Bunker, R.D]]
[[Category: Metcalf, P]]