5g4j - Revision history

OCA at 13:38, 26 July 2023

OCA — Wed, 26 Jul 2023 13:38:35 GMT

←Older revision		Revision as of 13:38, 26 July 2023
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	<StructureSection load='5g4j' size='340' side='right'caption='[[5g4j]], [[Resolution\|resolution]] 1.87Å' scene=''>		<StructureSection load='5g4j' size='340' side='right'caption='[[5g4j]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g4j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arthrobacter_aurescens_tc1 Arthrobacter~~ aurescens ~~tc1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G4J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5G4J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g4j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenarthrobacter_aurescens_TC1 Paenarthrobacter aurescens TC1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G4J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EXT:{5-HYDROXY-6-METHYL-4-[(E)-{[2-(PHOSPHONOOXY)ETHYL]IMINO}METHYL]PYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>EXT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.87Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g4i\|5g4i]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EXT:{5-HYDROXY-6-METHYL-4-[(E)-{[2-(PHOSPHONOOXY)ETHYL]IMINO}METHYL]PYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>EXT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ethanolamine-phosphate_phospho-lyase Ethanolamine-phosphate phospho-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.2 4.2.3.2] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g4j OCA], [https://pdbe.org/5g4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g4j RCSB], [https://www.ebi.ac.uk/pdbsum/5g4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g4j ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5g4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g4j OCA], [~~http~~://pdbe.org/5g4j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5g4j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5g4j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5g4j ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A1RDF1_PAEAT A1RDF1_PAEAT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arthrobacter aurescens tc1]]
-	[[Category: Ethanolamine-phosphate phospho-lyase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Cuetos, A~~]]	+	[[Category: Paenarthrobacter aurescens TC1]]
-	[[Category: ~~Grogan, G]]~~	+	[[Category: Cuetos A]]
-	~~[[Category: Sanchez, J Mangas]]~~	+	[[Category: Grogan G]]
-	~~[[Category: Tuan,~~ A N]]	+	[[Category: Mangas Sanchez J]]
-	[[Category: ~~Lyase~~]]	+	[[Category: Tuan AN]]
-	[[Category: ~~Phospholyase~~]]	+
-	[[Category: ~~Pyridoxal phosphate]]~~	+
-	~~[[Category: Transaminase~~]]	+

OCA at 06:38, 13 May 2020

OCA — Wed, 13 May 2020 06:38:41 GMT

←Older revision		Revision as of 06:38, 13 May 2020
Line 1:		Line 1:

	==Phospholyase A1RDF1 from Arthrobacter in complex with phosphoethanolamine==		==Phospholyase A1RDF1 from Arthrobacter in complex with phosphoethanolamine==
-	<StructureSection load='5g4j' size='340' side='right' caption='[[5g4j]], [[Resolution\|resolution]] 1.87Å' scene=''>	+	<StructureSection load='5g4j' size='340' side='right'caption='[[5g4j]], [[Resolution\|resolution]] 1.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G4J OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5G4J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g4j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_aurescens_tc1 Arthrobacter aurescens tc1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G4J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5G4J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EXT:{5-HYDROXY-6-METHYL-4-[(E)-{[2-(PHOSPHONOOXY)ETHYL]IMINO}METHYL]PYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>EXT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EXT:{5-HYDROXY-6-METHYL-4-[(E)-{[2-(PHOSPHONOOXY)ETHYL]IMINO}METHYL]PYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>EXT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g4i\|5g4i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g4i\|5g4i]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ethanolamine-phosphate_phospho-lyase Ethanolamine-phosphate phospho-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.2 4.2.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ethanolamine-phosphate_phospho-lyase Ethanolamine-phosphate phospho-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.2 4.2.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5g4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g4j OCA], [http://pdbe.org/5g4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g4j RCSB], [http://www.ebi.ac.uk/pdbsum/5g4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g4j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5g4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g4j OCA], [http://pdbe.org/5g4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g4j RCSB], [http://www.ebi.ac.uk/pdbsum/5g4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g4j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arthrobacter aurescens tc1]]
	[[Category: Ethanolamine-phosphate phospho-lyase]]		[[Category: Ethanolamine-phosphate phospho-lyase]]
		+	[[Category: Large Structures]]
	[[Category: Cuetos, A]]		[[Category: Cuetos, A]]
	[[Category: Grogan, G]]		[[Category: Grogan, G]]

OCA at 17:27, 19 October 2016

OCA — Wed, 19 Oct 2016 17:27:16 GMT

←Older revision		Revision as of 17:27, 19 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5g4j is ~~ON HOLD until Paper~~ Publication	+	==Phospholyase A1RDF1 from Arthrobacter in complex with phosphoethanolamine==
		+	<StructureSection load='5g4j' size='340' side='right' caption='[[5g4j]], [[Resolution\|resolution]] 1.87Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5g4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G4J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EXT:{5-HYDROXY-6-METHYL-4-[(E)-{[2-(PHOSPHONOOXY)ETHYL]IMINO}METHYL]PYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>EXT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g4i\|5g4i]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ethanolamine-phosphate_phospho-lyase Ethanolamine-phosphate phospho-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.2 4.2.3.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g4j OCA], [http://pdbe.org/5g4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g4j RCSB], [http://www.ebi.ac.uk/pdbsum/5g4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g4j ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PLP-dependent enzymes catalyze a remarkable diversity of chemical reactions in Nature. A1RDF1 from Arthrobacter aurescens TC1 is a Fold Type I, PLP-dependent enzyme in the Class III transaminase (TA) subgroup. Despite sharing 28% sequence identity with its closest structural homologs, including beta-alanine:pyruvate and gamma-amino butyrate:alpha-ketoglutarate TAs, A1RDF1 displayed no TA activity. Activity screening revealed the enzyme to possess phospholyase (E.C. 4.2.3.2) activity towards O-phosphoethanolamine (PEtN), an activity described previously for vertebrate enzymes such as human AGXT2L1, and for which no structure has yet been reported. In order to shed light on the distinctive features of PLP-dependent phospholyases, structures of A1RDF1 in complex with PLP (internal aldimine) and PLP-PEtN (external aldimine) were determined, revealing the basis of substrate binding and the structural factors that distinguish the enzyme from Class III homologs that display TA activity.

-	~~Authors: Cuetos, A~~., ~~Tuan,~~ A~~.N.~~, Mangas Sanchez, J., ~~Grogan~~, G.	+	Structural basis for phospholyase activity of a Class III transaminase homolog.,Cuetos A, Steffen-Munsberg F, Mangas Sanchez J, Frese A, Bornscheuer UT, Hohne M, Grogan G Chembiochem. 2016 Oct 6. doi: 10.1002/cbic.201600482. PMID:27709756<ref>PMID:27709756</ref>

-	~~Description~~: ~~Phospholyase A1RDF1 from Arthrobacter in complex with phosphoethanolamine~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5g4j" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ethanolamine-phosphate phospho-lyase]]
	[[Category: Cuetos, A]]		[[Category: Cuetos, A]]
-	[[Category: Mangas Sanchez, J]]
-	[[Category: Tuan, A.N]]
	[[Category: Grogan, G]]		[[Category: Grogan, G]]
		+	[[Category: Sanchez, J Mangas]]
		+	[[Category: Tuan, A N]]
		+	[[Category: Lyase]]
		+	[[Category: Phospholyase]]
		+	[[Category: Pyridoxal phosphate]]
		+	[[Category: Transaminase]]

OCA: Protected "5g4j" [edit=sysop:move=sysop]

OCA — Wed, 01 Jun 2016 11:39:57 GMT

Protected "5g4j" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:39, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5g4j is ON HOLD until Paper Publication Authors: Cuetos, A., Tuan, A.N., Mangas Sanchez, J., Grogan, G. Description: Phospholyase A1RDF1 from Arthr...

OCA — Wed, 01 Jun 2016 11:39:56 GMT

New page: '''Unreleased structure''' The entry 5g4j is ON HOLD until Paper Publication Authors: Cuetos, A., Tuan, A.N., Mangas Sanchez, J., Grogan, G. Description: Phospholyase A1RDF1 from Arthr...

New page

'''Unreleased structure'''

The entry 5g4j is ON HOLD until Paper Publication

Authors: Cuetos, A., Tuan, A.N., Mangas Sanchez, J., Grogan, G.

Description: Phospholyase A1RDF1 from Arthrobacter in complex with phosphoethanolamine
[[Category: Unreleased Structures]]
[[Category: Cuetos, A]]
[[Category: Mangas Sanchez, J]]
[[Category: Tuan, A.N]]
[[Category: Grogan, G]]