5g5c - Revision history

OCA at 13:39, 26 July 2023

OCA — Wed, 26 Jul 2023 13:39:27 GMT

←Older revision		Revision as of 13:39, 26 July 2023
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	<StructureSection load='5g5c' size='340' side='right'caption='[[5g5c]], [[Resolution\|resolution]] 1.18Å' scene=''>		<StructureSection load='5g5c' size='340' side='right'caption='[[5g5c]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g5c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_43587 Atcc 43587~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5G5C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g5c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G5C FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5g59\|5g59]]~~, [[~~5g5m~~\|~~5g5m~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.18Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5c OCA], [~~http~~://pdbe.org/5g5c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5g5c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5g5c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5c OCA], [https://pdbe.org/5g5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g5c RCSB], [https://www.ebi.ac.uk/pdbsum/5g5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5c ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8TZJ1_PYRFU Q8TZJ1_PYRFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 43587]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Reverter, D~~]]	+	[[Category: Pyrococcus furiosus]]
-	[[Category: ~~Varejao, N~~]]	+	[[Category: Reverter D]]
-	[[Category: ~~Esterase]]~~	+	[[Category: Varejao N]]
-	~~[[Category: Structural protein]]~~	+
-	~~[[Category: Themophilic~~]]	+

OCA at 06:39, 13 May 2020

OCA — Wed, 13 May 2020 06:39:38 GMT

←Older revision		Revision as of 06:39, 13 May 2020
Line 1:		Line 1:

	==Structure of the Pyrococcus furiosus Esterase Pf2001 with space group C2221==		==Structure of the Pyrococcus furiosus Esterase Pf2001 with space group C2221==
-	<StructureSection load='5g5c' size='340' side='right' caption='[[5g5c]], [[Resolution\|resolution]] 1.18Å' scene=''>	+	<StructureSection load='5g5c' size='340' side='right'caption='[[5g5c]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g5c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5C OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5G5C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g5c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5G5C FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g59\|5g59]], [[5g5m\|5g5m]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g59\|5g59]], [[5g5m\|5g5m]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5c OCA], [http://pdbe.org/5g5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g5c RCSB], [http://www.ebi.ac.uk/pdbsum/5g5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5c OCA], [http://pdbe.org/5g5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g5c RCSB], [http://www.ebi.ac.uk/pdbsum/5g5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5c ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 43587]]		[[Category: Atcc 43587]]
		+	[[Category: Large Structures]]
	[[Category: Reverter, D]]		[[Category: Reverter, D]]
	[[Category: Varejao, N]]		[[Category: Varejao, N]]

OCA at 08:05, 17 January 2018

OCA — Wed, 17 Jan 2018 08:05:43 GMT

←Older revision		Revision as of 08:05, 17 January 2018
Line 3:		Line 3:
	<StructureSection load='5g5c' size='340' side='right' caption='[[5g5c]], [[Resolution\|resolution]] 1.18Å' scene=''>		<StructureSection load='5g5c' size='340' side='right' caption='[[5g5c]], [[Resolution\|resolution]] 1.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5g5c]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G5C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5g5c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G5C FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g59\|5g59]], [[5g5m\|5g5m]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g59\|5g59]], [[5g5m\|5g5m]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5c OCA], [http://pdbe.org/5g5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g5c RCSB], [http://www.ebi.ac.uk/pdbsum/5g5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5c OCA], [http://pdbe.org/5g5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g5c RCSB], [http://www.ebi.ac.uk/pdbsum/5g5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5c ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lipases and esterases constitute a group of enzymes that catalyze the hydrolysis or synthesis of ester bonds. A major biotechnological interest corresponds to thermophilic esterases, due to their intrinsic stability at high temperatures. The Pf2001 esterase from Pyrococcus furiosus reaches its optimal activity between 70 degrees C and 80 degrees C. The crystal structure of the Pf2001 esterase shows two different conformations: monomer and dimer. The structures reveal important rearrangements in the "cap" subdomain between monomer and dimer, by the formation of an extensive intertwined helical interface. Moreover, the dimer interface is essential for the formation of the hydrophobic channel for substrate selectivity, as confirmed by mutagenesis and kinetic analysis. We also provide evidence for dimer formation at high temperatures, a process that correlates with its enzymatic activation. Thus, we propose a temperature-dependent activation mechanism of the Pf2001 esterase via dimerization that is necessary for the substrate channel formation in the active-site cleft.
		+
		+	Structural Mechanism for the Temperature-Dependent Activation of the Hyperthermophilic Pf2001 Esterase.,Varejao N, De-Andrade RA, Almeida RV, Anobom CD, Foguel D, Reverter D Structure. 2017 Dec 28. pii: S0969-2126(17)30403-3. doi:, 10.1016/j.str.2017.12.004. PMID:29307486<ref>PMID:29307486</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5g5c" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 43587]]
	[[Category: Reverter, D]]		[[Category: Reverter, D]]
	[[Category: Varejao, N]]		[[Category: Varejao, N]]

OCA at 06:53, 6 October 2017

OCA — Fri, 06 Oct 2017 06:53:50 GMT

←Older revision		Revision as of 06:53, 6 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5g5c is ~~ON HOLD until Paper Publication~~	+	==Structure of the Pyrococcus furiosus Esterase Pf2001 with space group C2221==
-		+	<StructureSection load='5g5c' size='340' side='right' caption='[[5g5c]], [[Resolution\|resolution]] 1.18Å' scene=''>
-	~~Authors~~: ~~Varejao~~, N., ~~Reverter~~, D.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5g5c]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G5C FirstGlance]. <br>
-	~~Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group C2221~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5g59\|5g59]], [[5g5m\|5g5m]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5c OCA], [http://pdbe.org/5g5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g5c RCSB], [http://www.ebi.ac.uk/pdbsum/5g5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5c ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Reverter, D]]
	[[Category: Varejao, N]]		[[Category: Varejao, N]]
-	[[Category: ~~Reverter, D~~]]	+	[[Category: Esterase]]
		+	[[Category: Structural protein]]
		+	[[Category: Themophilic]]

OCA at 06:34, 10 May 2017

OCA — Wed, 10 May 2017 06:34:00 GMT

←Older revision		Revision as of 06:34, 10 May 2017
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	Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group C2221		Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group C2221
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Reverter, D]]
	[[Category: Varejao, N]]		[[Category: Varejao, N]]
		+	[[Category: Reverter, D]]

OCA at 11:06, 20 June 2016

OCA — Mon, 20 Jun 2016 11:06:15 GMT

←Older revision		Revision as of 11:06, 20 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5g5c is ON HOLD	+	The entry 5g5c is ON HOLD until Paper Publication

	Authors: Varejao, N., Reverter, D.		Authors: Varejao, N., Reverter, D.

OCA: Protected "5g5c" [edit=sysop:move=sysop]

OCA — Wed, 01 Jun 2016 11:42:48 GMT

Protected "5g5c" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:42, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5g5c is ON HOLD Authors: Varejao, N., Reverter, D. Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group C2221 [[Categ...

OCA — Wed, 01 Jun 2016 11:42:47 GMT

New page: '''Unreleased structure''' The entry 5g5c is ON HOLD Authors: Varejao, N., Reverter, D. Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group C2221 [[Categ...

New page

'''Unreleased structure'''

The entry 5g5c is ON HOLD

Authors: Varejao, N., Reverter, D.

Description: Structure of the Pyrococcus Furiosus Esterase Pf2001 with space group C2221
[[Category: Unreleased Structures]]
[[Category: Reverter, D]]
[[Category: Varejao, N]]