5gly - Revision history

OCA at 11:35, 2 August 2023

OCA — Wed, 02 Aug 2023 11:35:41 GMT

←Older revision		Revision as of 11:35, 2 August 2023
Line 3:		Line 3:
	<StructureSection load='5gly' size='340' side='right'caption='[[5gly]], [[Resolution\|resolution]] 1.58Å' scene=''>		<StructureSection load='5gly' size='340' side='right'caption='[[5gly]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gly]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thett Thett~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GLY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GLY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gly]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothielavioides_terrestris_NRRL_8126 Thermothielavioides terrestris NRRL 8126]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GLY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene>~~, ~~<scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(~~1~~- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.58Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5glx\|5glx]], [[5gm9\|5gm9]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene>, <scene name='pdbligand=PRD_900014:alpha-cellotriose'>PRD_900014</scene></td></tr>
-	<~~tr id~~='~~gene~~'><~~td class="sblockLbl"~~><~~b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"~~>~~THITE_2110957 ([http:~~/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=578455 THETT])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gly OCA], [https://pdbe.org/5gly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gly RCSB], [https://www.ebi.ac.uk/pdbsum/5gly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gly ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5gly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gly OCA], [~~http~~://pdbe.org/5gly PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gly RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gly PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gly ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G2QVH7_THETT G2QVH7_THETT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thett~~]]	+	[[Category: Thermothielavioides terrestris NRRL 8126]]
-	[[Category: Chen~~, C C~~]]	+	[[Category: Chen CC]]
-	[[Category: Gao, J]]	+	[[Category: Gao J]]
-	[[Category: Guo~~, R T~~]]	+	[[Category: Guo RT]]
-	[[Category: Liu~~, W D~~]]	+	[[Category: Liu WD]]
-	[[Category: Zheng~~, Y Y]]~~	+	[[Category: Zheng YY]]
-	~~[[Category: Cellulase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Substrate binding~~]]	+

OCA at 06:46, 13 May 2020

OCA — Wed, 13 May 2020 06:46:16 GMT

←Older revision		Revision as of 06:46, 13 May 2020
Line 1:		Line 1:

	==Crystal structure of a glycoside hydrolase in complex with cellotetrose from Thielavia terrestris NRRL 8126==		==Crystal structure of a glycoside hydrolase in complex with cellotetrose from Thielavia terrestris NRRL 8126==
-	<StructureSection load='5gly' size='340' side='right' caption='[[5gly]], [[Resolution\|resolution]] 1.58Å' scene=''>	+	<StructureSection load='5gly' size='340' side='right'caption='[[5gly]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gly]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GLY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GLY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gly]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thett Thett]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GLY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GLY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5glx\|5glx]], [[5gm9\|5gm9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5glx\|5glx]], [[5gm9\|5gm9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gly OCA], [http://pdbe.org/5gly PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gly RCSB], [http://www.ebi.ac.uk/pdbsum/5gly PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gly ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THITE_2110957 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=578455 THETT])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gly OCA], [http://pdbe.org/5gly PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gly RCSB], [http://www.ebi.ac.uk/pdbsum/5gly PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gly ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Thett]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Gao, J]]		[[Category: Gao, J]]

OCA at 12:56, 19 April 2017

OCA — Wed, 19 Apr 2017 12:56:56 GMT

←Older revision		Revision as of 12:56, 19 April 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5gly is ~~ON HOLD until Jul 12 2018~~	+	==Crystal structure of a glycoside hydrolase in complex with cellotetrose from Thielavia terrestris NRRL 8126==
		+	<StructureSection load='5gly' size='340' side='right' caption='[[5gly]], [[Resolution\|resolution]] 1.58Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5gly]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GLY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GLY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5glx\|5glx]], [[5gm9\|5gm9]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gly OCA], [http://pdbe.org/5gly PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gly RCSB], [http://www.ebi.ac.uk/pdbsum/5gly PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gly ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	1,4-beta-Endoglucanase is one of the most important biocatalysts in modern industries. Here, a glycoside hydrolase (GH) family 45 endoglucanase from thermophilic fungus Theilavia terrestris (TtCel45A) was expressed in Pichia pastoris. The recombinant protein shows optimal activity at 60 degrees C, pH 4-5. The enzyme exhibits extraordinary thermostability that more than 80% activity was detected after heating at 80 degrees C for 2.5h. The high resolution crystal structures of apo-form enzyme and that in complex with cellobiose and cellotetraose were solved to 1.36-1.58A. The protein folds into two overall regions: one is a six-stranded beta-barrel, and the other one consists of several extended loops. Between the two regions lies the substrate-binding channel, which is an open cleft spanning across the protein surface. A continuous substrate-binding cleft from subsite -4 to +3 were clearly identified in the complex structures. Notably, the flexible V-VI loop (113Gly-114Gly-115Asp-116Leu-117Gly-118Ser) is found to open in the presence of -1 sugar, with D115 and L116 swung away to yield a space to accommodate the catalytic acid D122 and the 2,5B boat conformation of -1 sugar during transition state. Collectively, we characterized the enzyme properties of P. pastoris-expressed TtCel45A and solved high-resolution crystal structures of the enzyme. These results are of great interests in industrial applications and provide new insights into the fundamental understanding of enzyme catalytic mechanism of GH45 endoglucanases.

-	~~Authors: Gao~~, J., ~~Liu~~, W~~.D.~~, ~~Zheng~~, Y~~.Y.~~, Chen, C.C.~~, Guo, R~~.T.	+	Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-beta-endoglucanase from Thielavia terrestris.,Gao J, Huang JW, Li Q, Liu W, Ko TP, Zheng Y, Xiao X, Kuo CJ, Chen CC, Guo RT Enzyme Microb Technol. 2017 Apr;99:32-37. doi: 10.1016/j.enzmictec.2017.01.005., Epub 2017 Jan 17. PMID:28193329<ref>PMID:28193329</ref>

-	~~Description: Crystal structure~~ of ~~a glycoside hydrolase in complex with cellotetrose from Thielavia terrestris NRRL 8126~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Chen, C.C]]	+	<div class="pdbe-citations 5gly" style="background-color:#fffaf0;"></div>
-	~~[[Category: Liu, W.D]]~~	+	== References ==
-	~~[[Category: Guo, R.T~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, C C]]
	[[Category: Gao, J]]		[[Category: Gao, J]]
-	[[Category: Zheng, Y.Y]]	+	[[Category: Guo, R T]]
		+	[[Category: Liu, W D]]
		+	[[Category: Zheng, Y Y]]
		+	[[Category: Cellulase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Inhibitor]]
		+	[[Category: Substrate binding]]

OCA at 05:20, 12 April 2017

OCA — Wed, 12 Apr 2017 05:20:34 GMT

←Older revision		Revision as of 05:20, 12 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5gly is ON HOLD	+	The entry 5gly is ON HOLD until Jul 12 2018

	Authors: Gao, J., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.		Authors: Gao, J., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.

OCA: Protected "5gly" [edit=sysop:move=sysop]

OCA — Tue, 26 Jul 2016 09:45:17 GMT

Protected "5gly" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:45, 26 July 2016

OCA: New page: '''Unreleased structure''' The entry 5gly is ON HOLD Authors: Gao, J., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T. Description: Crystal structure of a glycoside hydrolase in complex ...

OCA — Tue, 26 Jul 2016 09:45:15 GMT

New page: '''Unreleased structure''' The entry 5gly is ON HOLD Authors: Gao, J., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T. Description: Crystal structure of a glycoside hydrolase in complex ...

New page

'''Unreleased structure'''

The entry 5gly is ON HOLD

Authors: Gao, J., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.

Description: Crystal structure of a glycoside hydrolase in complex with cellotetrose from Thielavia terrestris NRRL 8126
[[Category: Unreleased Structures]]
[[Category: Chen, C.C]]
[[Category: Liu, W.D]]
[[Category: Guo, R.T]]
[[Category: Gao, J]]
[[Category: Zheng, Y.Y]]