5gm4 - Revision history

OCA at 11:35, 2 August 2023

OCA — Wed, 02 Aug 2023 11:35:48 GMT

←Older revision		Revision as of 11:35, 2 August 2023
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	<StructureSection load='5gm4' size='340' side='right'caption='[[5gm4]], [[Resolution\|resolution]] 1.92Å' scene=''>		<StructureSection load='5gm4' size='340' side='right'caption='[[5gm4]], [[Resolution\|resolution]] 1.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gm4]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aspac Aspac~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GM4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GM4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gm4]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_aculeatus Aspergillus aculeatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GM4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CTT:BETA~~-~~D-GLUCOPYRANOSYL-(~~1~~- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.92Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5gm5\|5gm5]], [[5gm3\|5gm3]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gm4 OCA], [https://pdbe.org/5gm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gm4 RCSB], [https://www.ebi.ac.uk/pdbsum/5gm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gm4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5gm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gm4 OCA], [~~http~~://pdbe.org/5gm4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gm4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gm4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gm4 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GUN_ASPAC GUN_ASPAC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspac]]~~	+	[[Category: Aspergillus aculeatus]]
-	~~[[Category: Cellulase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen~~, C C~~]]	+	[[Category: Chen CC]]
-	[[Category: Guo~~, R T~~]]	+	[[Category: Guo RT]]
-	[[Category: Huang~~, J W~~]]	+	[[Category: Huang JW]]
-	[[Category: Liu~~, W D~~]]	+	[[Category: Liu WD]]
-	[[Category: Zheng~~, Y Y]]~~	+	[[Category: Zheng YY]]
-	~~[[Category: Hydrolase-inhibitor complex]]~~	+
-	~~[[Category: Substrate binding~~]]	+

OCA at 06:46, 13 May 2020

OCA — Wed, 13 May 2020 06:46:37 GMT

←Older revision		Revision as of 06:46, 13 May 2020
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	==Crystal structure of FI-CMCase from Aspergillus aculeatus F-50 in complex with cellotetrose==		==Crystal structure of FI-CMCase from Aspergillus aculeatus F-50 in complex with cellotetrose==
-	<StructureSection load='5gm4' size='340' side='right' caption='[[5gm4]], [[Resolution\|resolution]] 1.92Å' scene=''>	+	<StructureSection load='5gm4' size='340' side='right'caption='[[5gm4]], [[Resolution\|resolution]] 1.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gm4]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GM4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GM4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gm4]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspac Aspac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GM4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GM4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gm5\|5gm5]], [[5gm3\|5gm3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gm5\|5gm5]], [[5gm3\|5gm3]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gm4 OCA], [http://pdbe.org/5gm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gm4 RCSB], [http://www.ebi.ac.uk/pdbsum/5gm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gm4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gm4 OCA], [http://pdbe.org/5gm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gm4 RCSB], [http://www.ebi.ac.uk/pdbsum/5gm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gm4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5gm4" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5gm4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspac]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Guo, R T]]		[[Category: Guo, R T]]

OCA at 15:41, 17 May 2017

OCA — Wed, 17 May 2017 15:41:03 GMT

←Older revision		Revision as of 15:41, 17 May 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5gm4 is ~~ON HOLD until Jul~~ 12 ~~2018~~	+	==Crystal structure of FI-CMCase from Aspergillus aculeatus F-50 in complex with cellotetrose==
		+	<StructureSection load='5gm4' size='340' side='right' caption='[[5gm4]], [[Resolution\|resolution]] 1.92Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5gm4]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GM4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gm5\|5gm5]], [[5gm3\|5gm3]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gm4 OCA], [http://pdbe.org/5gm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gm4 RCSB], [http://www.ebi.ac.uk/pdbsum/5gm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gm4 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cellulose is the major component of the plant cell wall and the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in many commercial applications. Endo-1,4-beta-d-glucanase (EC 3.2.1.4; endoglucanase), which catalyzes the random hydrolysis of 1,4-beta-glycosidic bonds of the cellulose main chain to cleave cellulose into smaller fragments, is the key cellulolytic enzyme. An endoglucanase isolated from Aspergillus aculeatus F-50 (FI-CMCase), which is classified into the glycoside hydrolase (GH) family 12, was demonstrated to be effectively expressed in the industrial strain Pichia pastoris. Here, the crystal structure and complex structures of P. pastoris-expressed FI-CMCase were solved to high resolution. The overall structure is analyzed and compared to other GH12 members. In addition, the substrate-surrounding residues were engineered to search for variants with improved enzymatic activity. Among 14 mutants constructed, one with two-fold increase in protein expression was identified, which possesses a potential to be further developed as a commercial enzyme product.

-	~~Authors:~~ Huang, J.W., ~~Liu~~, ~~W.D.~~, Zheng, Y~~.Y.~~, ~~Chen~~, ~~C.C.~~, Guo, R.T.	+	Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50.,Huang JW, Liu W, Lai HL, Cheng YS, Zheng Y, Li Q, Sun H, Kuo CJ, Guo RT, Chen CC Biochem Biophys Res Commun. 2016 Sep 16;478(2):565-72. doi:, 10.1016/j.bbrc.2016.07.101. Epub 2016 Jul 25. PMID:27470581<ref>PMID:27470581</ref>

-	~~Description: Crystal structure of~~ a ~~cellulase in complex with cellotetrose~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Huang, J.W]]	+	<div class="pdbe-citations 5gm4" style="background-color:#fffaf0;"></div>
-	[[Category: Liu, W.D]]	+	== References ==
-	[[Category: ~~Chen~~, ~~C.C~~]]	+	<references/>
-	[[Category: ~~Guo, R.T~~]]	+	__TOC__
-	[[Category: ~~Zheng, Y.Y~~]]	+	</StructureSection>
		+	[[Category: Cellulase]]
		+	[[Category: Chen, C C]]
		+	[[Category: Guo, R T]]
		+	[[Category: Huang, J W]]
		+	[[Category: Liu, W D]]
		+	[[Category: Zheng, Y Y]]
		+	[[Category: Hydrolase-inhibitor complex]]
		+	[[Category: Substrate binding]]

OCA at 06:38, 10 May 2017

OCA — Wed, 10 May 2017 06:38:41 GMT

←Older revision		Revision as of 06:38, 10 May 2017
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	Description: Crystal structure of a cellulase in complex with cellotetrose		Description: Crystal structure of a cellulase in complex with cellotetrose
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Chen, C.C]]
	[[Category: Huang, J.W]]		[[Category: Huang, J.W]]
	[[Category: Liu, W.D]]		[[Category: Liu, W.D]]
		+	[[Category: Chen, C.C]]
	[[Category: Guo, R.T]]		[[Category: Guo, R.T]]
	[[Category: Zheng, Y.Y]]		[[Category: Zheng, Y.Y]]

OCA at 08:59, 9 March 2017

OCA — Thu, 09 Mar 2017 08:59:13 GMT

←Older revision		Revision as of 08:59, 9 March 2017
Line 7:		Line 7:
	Description: Crystal structure of a cellulase in complex with cellotetrose		Description: Crystal structure of a cellulase in complex with cellotetrose
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Huang, J.W]]
	[[Category: Chen, C.C]]		[[Category: Chen, C.C]]
		+	[[Category: Huang, J.W]]
	[[Category: Liu, W.D]]		[[Category: Liu, W.D]]
	[[Category: Guo, R.T]]		[[Category: Guo, R.T]]
	[[Category: Zheng, Y.Y]]		[[Category: Zheng, Y.Y]]

OCA at 23:16, 3 August 2016

OCA — Wed, 03 Aug 2016 23:16:00 GMT

←Older revision		Revision as of 23:16, 3 August 2016
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	The entry 5gm4 is ON HOLD until Jul 12 2018		The entry 5gm4 is ON HOLD until Jul 12 2018

-	Authors: Huang, C.W., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.	+	Authors: Huang, J.W., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.

	Description: Crystal structure of a cellulase in complex with cellotetrose		Description: Crystal structure of a cellulase in complex with cellotetrose
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Huang, J.W]]
	[[Category: Chen, C.C]]		[[Category: Chen, C.C]]
-	[[Category: Huang, C.W]]
	[[Category: Liu, W.D]]		[[Category: Liu, W.D]]
	[[Category: Guo, R.T]]		[[Category: Guo, R.T]]
	[[Category: Zheng, Y.Y]]		[[Category: Zheng, Y.Y]]

OCA at 10:35, 27 July 2016

OCA — Wed, 27 Jul 2016 10:35:44 GMT

←Older revision		Revision as of 10:35, 27 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5gm4 is ON HOLD	+	The entry 5gm4 is ON HOLD until Jul 12 2018

	Authors: Huang, C.W., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.		Authors: Huang, C.W., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.

OCA: Protected "5gm4" [edit=sysop:move=sysop]

OCA — Tue, 26 Jul 2016 09:45:52 GMT

Protected "5gm4" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:45, 26 July 2016

OCA: New page: '''Unreleased structure''' The entry 5gm4 is ON HOLD Authors: Huang, C.W., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T. Description: Crystal structure of a cellulase in complex with c...

OCA — Tue, 26 Jul 2016 09:45:50 GMT

New page: '''Unreleased structure''' The entry 5gm4 is ON HOLD Authors: Huang, C.W., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T. Description: Crystal structure of a cellulase in complex with c...

New page

'''Unreleased structure'''

The entry 5gm4 is ON HOLD

Authors: Huang, C.W., Liu, W.D., Zheng, Y.Y., Chen, C.C., Guo, R.T.

Description: Crystal structure of a cellulase in complex with cellotetrose
[[Category: Unreleased Structures]]
[[Category: Chen, C.C]]
[[Category: Huang, C.W]]
[[Category: Liu, W.D]]
[[Category: Guo, R.T]]
[[Category: Zheng, Y.Y]]