5goo - Revision history

OCA at 11:38, 2 August 2023

OCA — Wed, 02 Aug 2023 11:38:12 GMT

←Older revision		Revision as of 11:38, 2 August 2023
Line 3:		Line 3:
	<StructureSection load='5goo' size='340' side='right'caption='[[5goo]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='5goo' size='340' side='right'caption='[[5goo]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5goo]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Anabaena_7120 Anabaena~~ 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GOO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5goo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GOO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.11Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat">[[5gop\|5gop]], [[5goq\|5goq]], [[5gor\|5gor]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5goo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5goo OCA], [https://pdbe.org/5goo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5goo RCSB], [https://www.ebi.ac.uk/pdbsum/5goo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5goo ProSAT]</span></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">invA~~, ~~alr1521 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Beta-fructofuranosidase Beta-fructofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.26 3.2.1.26]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5goo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5goo OCA], [~~http~~://pdbe.org/5goo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5goo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5goo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5goo ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8YWS9_NOSS1 Q8YWS9_NOSS1]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Anabaena 7120]]
-	[[Category: Beta-fructofuranosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cai, K]]	+	[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
-	[[Category: Chen, Y]]	+	[[Category: Cai K]]
-	[[Category: Hu~~, H X~~]]	+	[[Category: Chen Y]]
-	[[Category: Hu~~, P F~~]]	+	[[Category: Hu HX]]
-	[[Category: Jiang~~, Y L~~]]	+	[[Category: Hu PF]]
-	[[Category: Xie, J]]	+	[[Category: Jiang YL]]
-	[[Category: Yang, F]]	+	[[Category: Xie J]]
-	[[Category: Zhou~~, C Z]]~~	+	[[Category: Yang F]]
-	~~[[Category: Alkaline invertase]]~~	+	[[Category: Zhou CZ]]
-	~~[[Category: Cyanobacteria]]~~	+
-	~~[[Category: Glycoside hydrolase family 100]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Sucrose hydrolysis~~]]	+

OCA at 06:48, 13 May 2020

OCA — Wed, 13 May 2020 06:48:43 GMT

←Older revision		Revision as of 06:48, 13 May 2020
Line 1:		Line 1:

	==Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with fructose==		==Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with fructose==
-	<StructureSection load='5goo' size='340' side='right' caption='[[5goo]], [[Resolution\|resolution]] 2.11Å' scene=''>	+	<StructureSection load='5goo' size='340' side='right'caption='[[5goo]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5goo]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GOO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5goo]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GOO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gop\|5gop]], [[5goq\|5goq]], [[5gor\|5gor]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gop\|5gop]], [[5goq\|5goq]], [[5gor\|5gor]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">invA, alr1521 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-fructofuranosidase Beta-fructofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.26 3.2.1.26] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-fructofuranosidase Beta-fructofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.26 3.2.1.26] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5goo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5goo OCA], [http://pdbe.org/5goo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5goo RCSB], [http://www.ebi.ac.uk/pdbsum/5goo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5goo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5goo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5goo OCA], [http://pdbe.org/5goo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5goo RCSB], [http://www.ebi.ac.uk/pdbsum/5goo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5goo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5goo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5goo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Invertase\|Invertase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Anabaena 7120]]
	[[Category: Beta-fructofuranosidase]]		[[Category: Beta-fructofuranosidase]]
		+	[[Category: Large Structures]]
	[[Category: Cai, K]]		[[Category: Cai, K]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]

OCA at 11:04, 15 December 2016

OCA — Thu, 15 Dec 2016 11:04:55 GMT

←Older revision		Revision as of 11:04, 15 December 2016
Line 10:		Line 10:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5goo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5goo OCA], [http://pdbe.org/5goo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5goo RCSB], [http://www.ebi.ac.uk/pdbsum/5goo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5goo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5goo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5goo OCA], [http://pdbe.org/5goo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5goo RCSB], [http://www.ebi.ac.uk/pdbsum/5goo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5goo ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Invertases catalyze the hydrolysis of sucrose to glucose and fructose, thereby playing a key role in primary metabolism and plant development. According to the optimum pH, invertases are classified into acid invertases (Ac-Invs) and alkaline/neutral invertases (A/N-Invs), which share no sequence homology. Compared with Ac-Invs that have been extensively studied, the structure and catalytic mechanism of A/N-Invs remain unknown. Here we report the crystal structures of Anabaena alkaline invertase InvA, which was proposed to be the ancestor of modern plant A/N-Invs. These structures are the first in the GH100 family. InvA exists as a hexamer in both crystal and solution. Each subunit consists of an (alpha/alpha)6 barrel core structure in addition to an insertion of three helices. A couple of structures in complex with the substrate or products enabled us to assign the subsites -1 and +1 specifically binding glucose and fructose, respectively. Structural comparison combined with enzymatic assays indicated that Asp-188 and Glu-414 are putative catalytic residues. Further analysis of the substrate binding pocket demonstrated that InvA possesses a stringent substrate specificity toward the alpha1,2-glycosidic bond of sucrose. Together, we suggest that InvA and homologs represent a novel family of glucosidases.
		+
		+	Structural Analysis of the Catalytic Mechanism and Substrate Specificity of Anabaena Alkaline Invertase InvA Reveals a Novel Glucosidase.,Xie J, Cai K, Hu HX, Jiang YL, Yang F, Hu PF, Cao DD, Li WF, Chen Y, Zhou CZ J Biol Chem. 2016 Dec 2;291(49):25667-25677. Epub 2016 Oct 24. PMID:27777307<ref>PMID:27777307</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5goo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 18:27, 2 November 2016

OCA — Wed, 02 Nov 2016 18:27:36 GMT

←Older revision		Revision as of 18:27, 2 November 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5goo ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with fructose==
-		+	<StructureSection load='5goo' size='340' side='right' caption='[[5goo]], [[Resolution\|resolution]] 2.11Å' scene=''>
-	~~Authors~~: ~~Xie, J~~., ~~Cai~~, K., ~~Hu, H~~.X.~~, Jiang, Y~~.L., ~~Yang~~, F., ~~Hu, P~~.F., ~~Chen, Y~~., ~~Zhou, C~~.Z.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5goo]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GOO FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with fructose~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gop\|5gop]], [[5goq\|5goq]], [[5gor\|5gor]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-fructofuranosidase Beta-fructofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.26 3.2.1.26] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5goo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5goo OCA], [http://pdbe.org/5goo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5goo RCSB], [http://www.ebi.ac.uk/pdbsum/5goo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5goo ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-fructofuranosidase]]
		+	[[Category: Cai, K]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
-	~~[[Category: Zhou, C.Z]]~~	+	[[Category: Hu, H X]]
-	~~[[Category: Cai, K]]~~	+	[[Category: Hu, P F]]
-	[[Category: Hu, H.X]]	+	[[Category: Jiang, Y L]]
-	[[Category: Hu, P.F]]	+
-	[[Category: Jiang, Y.L]]	+
	[[Category: Xie, J]]		[[Category: Xie, J]]
	[[Category: Yang, F]]		[[Category: Yang, F]]
		+	[[Category: Zhou, C Z]]
		+	[[Category: Alkaline invertase]]
		+	[[Category: Cyanobacteria]]
		+	[[Category: Glycoside hydrolase family 100]]
		+	[[Category: Hydrolase]]
		+	[[Category: Sucrose hydrolysis]]

OCA at 11:37, 19 October 2016

OCA — Wed, 19 Oct 2016 11:37:41 GMT

←Older revision		Revision as of 11:37, 19 October 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5goo is ON HOLD	+	The entry 5goo is ON HOLD until Paper Publication

	Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z.		Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z.

OCA at 16:00, 5 October 2016

OCA — Wed, 05 Oct 2016 16:00:15 GMT

←Older revision		Revision as of 16:00, 5 October 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5goo is ON HOLD ~~until Paper Publication~~	+	The entry 5goo is ON HOLD

	Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z.		Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z.

OCA at 11:52, 9 September 2016

OCA — Fri, 09 Sep 2016 11:52:54 GMT

←Older revision		Revision as of 11:52, 9 September 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5goo is ON HOLD	+	The entry 5goo is ON HOLD until Paper Publication

	Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z.		Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z.

OCA: Protected "5goo" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2016 23:22:04 GMT

Protected "5goo" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:22, 3 August 2016

OCA: New page: '''Unreleased structure''' The entry 5goo is ON HOLD Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z. Description: Crystal structure of alkali...

OCA — Wed, 03 Aug 2016 23:22:02 GMT

New page: '''Unreleased structure''' The entry 5goo is ON HOLD Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z. Description: Crystal structure of alkali...

New page

'''Unreleased structure'''

The entry 5goo is ON HOLD

Authors: Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Chen, Y., Zhou, C.Z.

Description: Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with fructose
[[Category: Unreleased Structures]]
[[Category: Chen, Y]]
[[Category: Zhou, C.Z]]
[[Category: Cai, K]]
[[Category: Hu, H.X]]
[[Category: Hu, P.F]]
[[Category: Jiang, Y.L]]
[[Category: Xie, J]]
[[Category: Yang, F]]