5gpd - Revision history

OCA at 09:13, 20 March 2024

OCA — Wed, 20 Mar 2024 09:13:19 GMT

←Older revision		Revision as of 09:13, 20 March 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SREBP_SCHPO SREBP_SCHPO] Transcriptional activator required for transcription of genes required for adaptation to anaerobic growth like those implicated in the nonrespiratory oxygen-consumptive biosynthetic pathways of sterol, heme, sphingolipid, and ubiquinone biosynthesis. May monitor oxygen levels through sterol synthesis steps which require oxygen.<ref>PMID:15797383</ref> <ref>PMID:16537923</ref> <ref>PMID:18276645</ref>		[https://www.uniprot.org/uniprot/SREBP_SCHPO SREBP_SCHPO] Transcriptional activator required for transcription of genes required for adaptation to anaerobic growth like those implicated in the nonrespiratory oxygen-consumptive biosynthetic pathways of sterol, heme, sphingolipid, and ubiquinone biosynthesis. May monitor oxygen levels through sterol synthesis steps which require oxygen.<ref>PMID:15797383</ref> <ref>PMID:16537923</ref> <ref>PMID:18276645</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Sterol regulatory element-binding protein (SREBP) transcription factors are master regulators of cellular lipid homeostasis in mammals and oxygen-responsive regulators of hypoxic adaptation in fungi. SREBP C-terminus binds to the WD40 domain of SREBP cleavage-activating protein (SCAP), which confers sterol regulation by controlling the ER-to-Golgi transport of the SREBP-SCAP complex and access to the activating proteases in the Golgi. Here, we biochemically and structurally show that the carboxyl terminal domains (CTD) of Sre1 and Scp1, the fission yeast SREBP and SCAP, form a functional 4:4 oligomer and Sre1-CTD forms a dimer of dimers. The crystal structure of Sre1-CTD at 3.5 A and cryo-EM structure of the complex at 5.4 A together with in vitro biochemical evidence elucidate three distinct regions in Sre1-CTD required for Scp1 binding, Sre1-CTD dimerization and tetrameric formation. Finally, these structurally identified domains are validated in a cellular context, demonstrating that the proper 4:4 oligomeric complex formation is required for Sre1 activation.
-
-	Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization.,Gong X, Qian H, Shao W, Li J, Wu J, Liu JJ, Li W, Wang HW, Espenshade P, Yan N Cell Res. 2016 Nov;26(11):1197-1211. doi: 10.1038/cr.2016.123. Epub 2016 Nov 4. PMID:27811944<ref>PMID:27811944</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5gpd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:38, 2 August 2023

OCA — Wed, 02 Aug 2023 11:38:42 GMT

←Older revision		Revision as of 11:38, 2 August 2023
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	<StructureSection load='5gpd' size='340' side='right'caption='[[5gpd]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='5gpd' size='340' side='right'caption='[[5gpd]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gpd]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Fission_yeast Fission yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GPD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gpd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GPD FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~sre1~~, ~~SPBC19C2.09 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=284812 Fission yeast])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.501Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5gpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpd OCA], [~~http~~://pdbe.org/5gpd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gpd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gpd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpd OCA], [https://pdbe.org/5gpd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gpd RCSB], [https://www.ebi.ac.uk/pdbsum/5gpd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SREBP_SCHPO SREBP_SCHPO]] Transcriptional activator required for transcription of genes required for adaptation to anaerobic growth like those implicated in the nonrespiratory oxygen-consumptive biosynthetic pathways of sterol, heme, sphingolipid, and ubiquinone biosynthesis. May monitor oxygen levels through sterol synthesis steps which require oxygen.<ref>PMID:15797383</ref> <ref>PMID:16537923</ref> <ref>PMID:18276645</ref>	+	[https://www.uniprot.org/uniprot/SREBP_SCHPO SREBP_SCHPO] Transcriptional activator required for transcription of genes required for adaptation to anaerobic growth like those implicated in the nonrespiratory oxygen-consumptive biosynthetic pathways of sterol, heme, sphingolipid, and ubiquinone biosynthesis. May monitor oxygen levels through sterol synthesis steps which require oxygen.<ref>PMID:15797383</ref> <ref>PMID:16537923</ref> <ref>PMID:18276645</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fission yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gong, X~~]]	+	[[Category: Schizosaccharomyces pombe 972h-]]
-	[[Category: ~~Qian, H W~~]]	+	[[Category: Gong X]]
-	[[Category: ~~Wu, J P~~]]	+	[[Category: Qian HW]]
-	[[Category: ~~Yan, N~~]]	+	[[Category: Wu JP]]
-	[[Category: ~~Dna binding protein]]~~	+	[[Category: Yan N]]
-	~~[[Category: Regulation domain~~]]	+

OCA at 06:49, 13 May 2020

OCA — Wed, 13 May 2020 06:49:26 GMT

←Older revision		Revision as of 06:49, 13 May 2020
Line 1:		Line 1:

	==Crystal structure of the binding domain of SREBP from fission yeast==		==Crystal structure of the binding domain of SREBP from fission yeast==
-	<StructureSection load='5gpd' size='340' side='right' caption='[[5gpd]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='5gpd' size='340' side='right'caption='[[5gpd]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gpd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPD OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GPD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gpd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GPD FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpd OCA], [http://pdbe.org/5gpd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpd RCSB], [http://www.ebi.ac.uk/pdbsum/5gpd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpd ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sre1, SPBC19C2.09 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpd OCA], [http://pdbe.org/5gpd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpd RCSB], [http://www.ebi.ac.uk/pdbsum/5gpd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fission yeast]]
		+	[[Category: Large Structures]]
	[[Category: Gong, X]]		[[Category: Gong, X]]
	[[Category: Qian, H W]]		[[Category: Qian, H W]]

OCA at 10:53, 10 December 2016

OCA — Sat, 10 Dec 2016 10:53:00 GMT

←Older revision		Revision as of 10:53, 10 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5gpd is ~~ON HOLD~~	+	==Crystal structure of the binding domain of SREBP from fission yeast==
		+	<StructureSection load='5gpd' size='340' side='right' caption='[[5gpd]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5gpd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GPD FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gpd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpd OCA], [http://pdbe.org/5gpd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpd RCSB], [http://www.ebi.ac.uk/pdbsum/5gpd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpd ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SREBP_SCHPO SREBP_SCHPO]] Transcriptional activator required for transcription of genes required for adaptation to anaerobic growth like those implicated in the nonrespiratory oxygen-consumptive biosynthetic pathways of sterol, heme, sphingolipid, and ubiquinone biosynthesis. May monitor oxygen levels through sterol synthesis steps which require oxygen.<ref>PMID:15797383</ref> <ref>PMID:16537923</ref> <ref>PMID:18276645</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sterol regulatory element-binding protein (SREBP) transcription factors are master regulators of cellular lipid homeostasis in mammals and oxygen-responsive regulators of hypoxic adaptation in fungi. SREBP C-terminus binds to the WD40 domain of SREBP cleavage-activating protein (SCAP), which confers sterol regulation by controlling the ER-to-Golgi transport of the SREBP-SCAP complex and access to the activating proteases in the Golgi. Here, we biochemically and structurally show that the carboxyl terminal domains (CTD) of Sre1 and Scp1, the fission yeast SREBP and SCAP, form a functional 4:4 oligomer and Sre1-CTD forms a dimer of dimers. The crystal structure of Sre1-CTD at 3.5 A and cryo-EM structure of the complex at 5.4 A together with in vitro biochemical evidence elucidate three distinct regions in Sre1-CTD required for Scp1 binding, Sre1-CTD dimerization and tetrameric formation. Finally, these structurally identified domains are validated in a cellular context, demonstrating that the proper 4:4 oligomeric complex formation is required for Sre1 activation.

-	~~Authors~~:	+	Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization.,Gong X, Qian H, Shao W, Li J, Wu J, Liu JJ, Li W, Wang HW, Espenshade P, Yan N Cell Res. 2016 Nov;26(11):1197-1211. doi: 10.1038/cr.2016.123. Epub 2016 Nov 4. PMID:27811944<ref>PMID:27811944</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5gpd" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gong, X]]
		+	[[Category: Qian, H W]]
		+	[[Category: Wu, J P]]
		+	[[Category: Yan, N]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Regulation domain]]

OCA: Protected "5gpd" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2016 11:09:06 GMT

Protected "5gpd" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:09, 10 August 2016

OCA: New page: '''Unreleased structure''' The entry 5gpd is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Aug 2016 11:09:05 GMT

New page: '''Unreleased structure''' The entry 5gpd is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5gpd is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]