5gpo - Revision history

OCA at 09:13, 20 March 2024

OCA — Wed, 20 Mar 2024 09:13:19 GMT

←Older revision		Revision as of 09:13, 20 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q9I0V9_PSEAE Q9I0V9_PSEAE] Member of a two-component regulatory system.[RuleBase:RU364088]		[https://www.uniprot.org/uniprot/Q9I0V9_PSEAE Q9I0V9_PSEAE] Member of a two-component regulatory system.[RuleBase:RU364088]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Pseudomonas aeruginosa (P. aeruginosa) is a major opportunistic human pathogen, causing serious nosocomial infections among immunocompromised patients by multi-determinant virulence and high antibiotic resistance. The CzcR-CzcS signal transduction system in P. aeruginosa is primarily involved in metal detoxification and antibiotic resistance through co-regulating cross-resistance between Zn(II) and carbapenem antibiotics. Although the intracellular regulatory pathway is well-established, the mechanism by which extracellular sensor domain of histidine kinase (HK) CzcS responds to Zn(II) stimulus to trigger downstream signal transduction remains unclear. Here we determined the crystal structure of the CzcS sensor domain (CzcS SD) in complex with Zn(II) at 1.7 A resolution. This is the first three-dimensional structural view of Zn(II)-sensor domain of the two-component system (TCS). The CzcS SD is of alpha/beta-fold in nature, and it senses the Zn(II) stimulus at micromole level in a tetrahedral geometry through its symmetry-related residues (His55 and Asp60) on the dimer interface. Though the CzcS SD resembles the PhoQ-DcuS-CitA (PDC) superfamily member, it interacts with the effector in a novel domain with the N-terminal alpha-helices rather than the conserved beta-sheets pocket. The dimerization of the N-terminal H1 and H1' alpha-helices is of primary importance for the activity of HK CzcS. This study provides preliminary insight into the molecular mechanism of Zn(II) sensing and signaling transduction by the HK CzcS, which will be beneficial to understand how the pathogen P. aeruginosa resists to high levels of heavy metals and antimicrobial agents.
-
-	Structural basis of Zn(II) induced metal detoxification and antibiotic resistance by histidine kinase CzcS in Pseudomonas aeruginosa.,Wang D, Chen W, Huang S, He Y, Liu X, Hu Q, Wei T, Sang H, Gan J, Chen H PLoS Pathog. 2017 Jul 21;13(7):e1006533. doi: 10.1371/journal.ppat.1006533., eCollection 2017 Jul. PMID:28732057<ref>PMID:28732057</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5gpo" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:38, 2 August 2023

OCA — Wed, 02 Aug 2023 11:38:57 GMT

←Older revision		Revision as of 11:38, 2 August 2023
Line 3:		Line 3:
	<StructureSection load='5gpo' size='340' side='right'caption='[[5gpo]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5gpo' size='340' side='right'caption='[[5gpo]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gpo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gpo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GPO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.701Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Histidine_kinase Histidine kinase]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5gpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpo OCA], [~~http~~://pdbe.org/5gpo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gpo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gpo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpo OCA], [https://pdbe.org/5gpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gpo RCSB], [https://www.ebi.ac.uk/pdbsum/5gpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9I0V9_PSEAE Q9I0V9_PSEAE] Member of a two-component regulatory system.[RuleBase:RU364088]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Histidine kinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, H]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Chen~~, W Z~~]]	+	[[Category: Chen H]]
-	[[Category: Gan~~, J H~~]]	+	[[Category: Chen WZ]]
-	[[Category: Hu~~, Q Y~~]]	+	[[Category: Gan JH]]
-	[[Category: Huang~~, S Q~~]]	+	[[Category: Hu QY]]
-	[[Category: Liu~~, X C~~]]	+	[[Category: Huang SQ]]
-	[[Category: Wang, D]]	+	[[Category: Liu XC]]
-	[[Category: Wei~~, T B]]~~	+	[[Category: Wang D]]
-	~~[[Category: Heavy metal]]~~	+	[[Category: Wei TB]]
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Two-component system~~]]	+

OCA at 06:49, 13 May 2020

OCA — Wed, 13 May 2020 06:49:40 GMT

←Older revision		Revision as of 06:49, 13 May 2020
Line 1:		Line 1:

	==The sensor domain structure of the zinc-responsive histidine kinase CzcS from Pseudomonas Aeruginosa==		==The sensor domain structure of the zinc-responsive histidine kinase CzcS from Pseudomonas Aeruginosa==
-	<StructureSection load='5gpo' size='340' side='right' caption='[[5gpo]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5gpo' size='340' side='right'caption='[[5gpo]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gpo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gpo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GPO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpo OCA], [http://pdbe.org/5gpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpo RCSB], [http://www.ebi.ac.uk/pdbsum/5gpo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpo OCA], [http://pdbe.org/5gpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpo RCSB], [http://www.ebi.ac.uk/pdbsum/5gpo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
		+	[[Category: Large Structures]]
	[[Category: Chen, H]]		[[Category: Chen, H]]
	[[Category: Chen, W Z]]		[[Category: Chen, W Z]]

OCA at 09:14, 9 August 2017

OCA — Wed, 09 Aug 2017 09:14:50 GMT

←Older revision		Revision as of 09:14, 9 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5gpo is ~~ON HOLD until Paper~~ Publication	+	==The sensor domain structure of the zinc-responsive histidine kinase CzcS from Pseudomonas Aeruginosa==
		+	<StructureSection load='5gpo' size='340' side='right' caption='[[5gpo]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5gpo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GPO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gpo OCA], [http://pdbe.org/5gpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gpo RCSB], [http://www.ebi.ac.uk/pdbsum/5gpo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gpo ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudomonas aeruginosa (P. aeruginosa) is a major opportunistic human pathogen, causing serious nosocomial infections among immunocompromised patients by multi-determinant virulence and high antibiotic resistance. The CzcR-CzcS signal transduction system in P. aeruginosa is primarily involved in metal detoxification and antibiotic resistance through co-regulating cross-resistance between Zn(II) and carbapenem antibiotics. Although the intracellular regulatory pathway is well-established, the mechanism by which extracellular sensor domain of histidine kinase (HK) CzcS responds to Zn(II) stimulus to trigger downstream signal transduction remains unclear. Here we determined the crystal structure of the CzcS sensor domain (CzcS SD) in complex with Zn(II) at 1.7 A resolution. This is the first three-dimensional structural view of Zn(II)-sensor domain of the two-component system (TCS). The CzcS SD is of alpha/beta-fold in nature, and it senses the Zn(II) stimulus at micromole level in a tetrahedral geometry through its symmetry-related residues (His55 and Asp60) on the dimer interface. Though the CzcS SD resembles the PhoQ-DcuS-CitA (PDC) superfamily member, it interacts with the effector in a novel domain with the N-terminal alpha-helices rather than the conserved beta-sheets pocket. The dimerization of the N-terminal H1 and H1' alpha-helices is of primary importance for the activity of HK CzcS. This study provides preliminary insight into the molecular mechanism of Zn(II) sensing and signaling transduction by the HK CzcS, which will be beneficial to understand how the pathogen P. aeruginosa resists to high levels of heavy metals and antimicrobial agents.

-	~~Authors:~~ Wang, D., Chen, W~~.Z.~~, Huang, S~~.Q.~~, Liu, X~~.C.~~, Hu, Q~~.Y.~~, Wei, T~~.B.~~, Gan, J~~.H.~~, Chen, H.	+	Structural basis of Zn(II) induced metal detoxification and antibiotic resistance by histidine kinase CzcS in Pseudomonas aeruginosa.,Wang D, Chen W, Huang S, He Y, Liu X, Hu Q, Wei T, Sang H, Gan J, Chen H PLoS Pathog. 2017 Jul 21;13(7):e1006533. doi: 10.1371/journal.ppat.1006533., eCollection 2017 Jul. PMID:28732057<ref>PMID:28732057</ref>

-	~~Description: The sensor domain structure~~ of the ~~zinc-responsive histidine kinase CzcS from Pseudomonas Aeruginosa~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Hu, Q.Y]]~~	+	<div class="pdbe-citations 5gpo" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Wang, D~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Histidine kinase]]
	[[Category: Chen, H]]		[[Category: Chen, H]]
-	[[Category: ~~Liu~~, ~~X.C~~]]	+	[[Category: Chen, W Z]]
-	[[Category: Gan, J.H]]	+	[[Category: Gan, J H]]
-	[[Category: ~~Chen~~, ~~W.Z~~]]	+	[[Category: Hu, Q Y]]
-	[[Category: Wei, T.B]]	+	[[Category: Huang, S Q]]
-	[[Category: ~~Huang, S.Q~~]]	+	[[Category: Liu, X C]]
		+	[[Category: Wang, D]]
		+	[[Category: Wei, T B]]
		+	[[Category: Heavy metal]]
		+	[[Category: Transferase]]
		+	[[Category: Two-component system]]

OCA at 09:02, 9 March 2017

OCA — Thu, 09 Mar 2017 09:02:38 GMT

←Older revision		Revision as of 09:02, 9 March 2017
Line 9:		Line 9:
	[[Category: Hu, Q.Y]]		[[Category: Hu, Q.Y]]
	[[Category: Wang, D]]		[[Category: Wang, D]]
-	[[Category: Liu, X.C]]
-	[[Category: Wei, T.B]]
	[[Category: Chen, H]]		[[Category: Chen, H]]
		+	[[Category: Liu, X.C]]
	[[Category: Gan, J.H]]		[[Category: Gan, J.H]]
	[[Category: Chen, W.Z]]		[[Category: Chen, W.Z]]
		+	[[Category: Wei, T.B]]
	[[Category: Huang, S.Q]]		[[Category: Huang, S.Q]]

OCA at 11:54, 9 September 2016

OCA — Fri, 09 Sep 2016 11:54:52 GMT

←Older revision		Revision as of 11:54, 9 September 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5gpo is ON HOLD	+	The entry 5gpo is ON HOLD until Paper Publication

	Authors: Wang, D., Chen, W.Z., Huang, S.Q., Liu, X.C., Hu, Q.Y., Wei, T.B., Gan, J.H., Chen, H.		Authors: Wang, D., Chen, W.Z., Huang, S.Q., Liu, X.C., Hu, Q.Y., Wei, T.B., Gan, J.H., Chen, H.

OCA: Protected "5gpo" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2016 11:10:30 GMT

Protected "5gpo" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:10, 10 August 2016

OCA: New page: '''Unreleased structure''' The entry 5gpo is ON HOLD Authors: Wang, D., Chen, W.Z., Huang, S.Q., Liu, X.C., Hu, Q.Y., Wei, T.B., Gan, J.H., Chen, H. Description: The sensor domain stru...

OCA — Wed, 10 Aug 2016 11:10:29 GMT

New page: '''Unreleased structure''' The entry 5gpo is ON HOLD Authors: Wang, D., Chen, W.Z., Huang, S.Q., Liu, X.C., Hu, Q.Y., Wei, T.B., Gan, J.H., Chen, H. Description: The sensor domain stru...

New page

'''Unreleased structure'''

The entry 5gpo is ON HOLD

Authors: Wang, D., Chen, W.Z., Huang, S.Q., Liu, X.C., Hu, Q.Y., Wei, T.B., Gan, J.H., Chen, H.

Description: The sensor domain structure of the zinc-responsive histidine kinase CzcS from Pseudomonas Aeruginosa
[[Category: Unreleased Structures]]
[[Category: Hu, Q.Y]]
[[Category: Wang, D]]
[[Category: Liu, X.C]]
[[Category: Wei, T.B]]
[[Category: Chen, H]]
[[Category: Gan, J.H]]
[[Category: Chen, W.Z]]
[[Category: Huang, S.Q]]