5gwo - Revision history

OCA at 11:44, 2 August 2023

OCA — Wed, 02 Aug 2023 11:44:50 GMT

←Older revision		Revision as of 11:44, 2 August 2023
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	<StructureSection load='5gwo' size='340' side='right'caption='[[5gwo]], [[Resolution\|resolution]] 2.82Å' scene=''>		<StructureSection load='5gwo' size='340' side='right'caption='[[5gwo]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gwo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GWO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gwo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa] and [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GWO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.816Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gwp\|5gwp]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwo OCA], [https://pdbe.org/5gwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gwo RCSB], [https://www.ebi.ac.uk/pdbsum/5gwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwo ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5gwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwo OCA], [~~http~~://pdbe.org/5gwo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gwo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gwo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwo ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/P2C50_ORYSJ P2C50_ORYSJ]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phosphoprotein phosphatase~~]]	+	[[Category: Oryza sativa]]
-	[[Category: ~~Han, S~~]]	+	[[Category: Oryza sativa Japonica Group]]
-	[[Category: ~~Lee,~~ S]]	+	[[Category: Han S]]
-	[[Category: ~~Aba receptor]]~~	+	[[Category: Lee S]]
-	~~[[Category: Abscisic acid]]~~	+
-	~~[[Category: Hydrolase-receptor complex]]~~	+
-	~~[[Category: Pp2c~~]]	+

OCA at 06:45, 20 May 2020

OCA — Wed, 20 May 2020 06:45:16 GMT

←Older revision		Revision as of 06:45, 20 May 2020
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	==Crystal structure of RCAR3:PP2C S265F/I267M with (+)-ABA==		==Crystal structure of RCAR3:PP2C S265F/I267M with (+)-ABA==
-	<StructureSection load='5gwo' size='340' side='right' caption='[[5gwo]], [[Resolution\|resolution]] 2.82Å' scene=''>	+	<StructureSection load='5gwo' size='340' side='right'caption='[[5gwo]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gwo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GWO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gwo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GWO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gwp\|5gwp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gwp\|5gwp]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwo OCA], [http://pdbe.org/5gwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gwo RCSB], [http://www.ebi.ac.uk/pdbsum/5gwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwo OCA], [http://pdbe.org/5gwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gwo RCSB], [http://www.ebi.ac.uk/pdbsum/5gwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
	[[Category: Han, S]]		[[Category: Han, S]]

OCA at 10:39, 13 September 2017

OCA — Wed, 13 Sep 2017 10:39:08 GMT

←Older revision		Revision as of 10:39, 13 September 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5gwo is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of RCAR3:PP2C S265F/I267M with (+)-ABA==
		+	<StructureSection load='5gwo' size='340' side='right' caption='[[5gwo]], [[Resolution\|resolution]] 2.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5gwo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GWO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gwp\|5gwp]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwo OCA], [http://pdbe.org/5gwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gwo RCSB], [http://www.ebi.ac.uk/pdbsum/5gwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwo ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Abscisic acid (ABA) signaling pathway is regulated by Clade A type 2C protein phosphatases (PP2CAs) in plant. In the presence of ABA, PP2Cs release stress/ABA-activated protein kinases by binding to ABA-bound receptors (PYL/RCARs) for activation. Although the wedging tryptophan in PP2Cs is critical in the interaction with PYL/RCARs in Arabidopsis and rice, it remains elusive how other interface regions are involved in the interaction. Here, we identified a conserved region on PP2Cs, termed VxGPhiL motif, that modulates the interaction with PYL/RCARs through its second and forth residues. The effects of the second and forth residues on the interaction of OsPP2C50 with several OsPYL/RCAR proteins were investigated by systematic mutagenesis. Investigating the interaction between OsPP2C50 and OsPYL/RCAR3, one OsPP2C50 mutant, VFGML ("FM") mutant, lowered the affinity by approximately 15-fold. Comparison of the crystal structures of OsPP2C50:ABA:OsPYL/RCAR3 wild-type and FM mutant revealed local conformational changes near the VxGPhiL motif, further supported by hydrogen-deuterium exchange mass spectrometry. In rice protoplasts, ABA signaling was altered by mutations in the VxGPhiL motif. Transgenic Arabidopsis plants overexpressing OsPP2C50 and OsPP2C50FM changed ABA sensitivity, validating the in vitro results. Taken together, the VxGPhiL motif of PP2Cs seems to modulate the affinity to PYL/RCARs with potentials to alter the ABA signaling, leading to the differential sensitivity to ABA in planta.

-	~~Authors~~:	+	Modulation of ABA signaling by altering VxGPhiL motif of PP2Cs in Oryza sativa.,Han S, Min MK, Lee SY, Lim CW, Bhatnagar N, Lee Y, Shin D, Chung KY, Lee SC, Kim BG, Lee S Mol Plant. 2017 Aug 18. pii: S1674-2052(17)30233-2. doi:, 10.1016/j.molp.2017.08.003. PMID:28827170<ref>PMID:28827170</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5gwo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Phosphoprotein phosphatase]]
		+	[[Category: Han, S]]
		+	[[Category: Lee, S]]
		+	[[Category: Aba receptor]]
		+	[[Category: Abscisic acid]]
		+	[[Category: Hydrolase-receptor complex]]
		+	[[Category: Pp2c]]

OCA at 11:30, 3 October 2016

OCA — Mon, 03 Oct 2016 11:30:42 GMT

←Older revision		Revision as of 11:30, 3 October 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5gwo is ON HOLD	+	The entry 5gwo is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5gwo" [edit=sysop:move=sysop]

OCA — Wed, 21 Sep 2016 08:43:48 GMT

Protected "5gwo" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:43, 21 September 2016

OCA: New page: '''Unreleased structure''' The entry 5gwo is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Sep 2016 08:43:47 GMT

New page: '''Unreleased structure''' The entry 5gwo is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5gwo is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]