5gwr - Revision history

OCA at 11:44, 2 August 2023

OCA — Wed, 02 Aug 2023 11:44:55 GMT

←Older revision		Revision as of 11:44, 2 August 2023
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	==4-hydroxyisoleucine dehydrogenase complexed with NADH==		==4-hydroxyisoleucine dehydrogenase complexed with NADH==
-	<StructureSection load='5gwr' size='340' side='right' caption='[[5gwr]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='5gwr' size='340' side='right'caption='[[5gwr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gwr]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var.~~ thuringiensis~~" smith et al. 1952~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GWR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gwr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GWR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5gws\|5gws]]~~, ~~[[5gwt\|5gwt]]</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">AC241_05390 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1428 "Bacillus cereus var. thuringiensis" Smith et al. 1952])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwr OCA], [https://pdbe.org/5gwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gwr RCSB], [https://www.ebi.ac.uk/pdbsum/5gwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwr ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwr OCA], [~~http~~://pdbe.org/5gwr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gwr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gwr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwr ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0K0Q8K4_BACTU A0A0K0Q8K4_BACTU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~cereus var.~~ thuringiensis ~~smith et al. 1952~~]]	+	[[Category: Bacillus thuringiensis]]
-	[[Category: Miyakawa, T]]	+	[[Category: Large Structures]]
-	[[Category: Nakamura, A]]	+	[[Category: Miyakawa T]]
-	[[Category: Shi, X]]	+	[[Category: Nakamura A]]
-	[[Category: Tanokura, M]]	+	[[Category: Shi X]]
-	~~[[Category: Dehydrogenase]]~~	+	[[Category: Tanokura M]]
-	~~[[Category: Nadh-dependent]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Reductase]]~~	+
-	~~[[Category: Short-chain~~]]	+

OCA at 07:35, 8 November 2017

OCA — Wed, 08 Nov 2017 07:35:37 GMT

←Older revision		Revision as of 07:35, 8 November 2017
Line 3:		Line 3:
	<StructureSection load='5gwr' size='340' side='right' caption='[[5gwr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='5gwr' size='340' side='right' caption='[[5gwr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gwr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GWR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gwr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GWR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gws\|5gws]], [[5gwt\|5gwt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gws\|5gws]], [[5gwt\|5gwt]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AC241_05390 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1428 "Bacillus cereus var. thuringiensis" Smith et al. 1952])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwr OCA], [http://pdbe.org/5gwr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gwr RCSB], [http://www.ebi.ac.uk/pdbsum/5gwr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwr OCA], [http://pdbe.org/5gwr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gwr RCSB], [http://www.ebi.ac.uk/pdbsum/5gwr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwr ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fenugreek is a dietary supplement for anti-aging and human health. (2S,3R,4S)-4-hydroxyisoleucine (4-HIL), which is extracted from fenugreek seeds, is expected to be a promising orally active drug for diabetes and diabetic nephropathy because of its insulinotropic effect. Although several chemical synthesis methods of 4-HIL have been proposed, these methods require multistep reactions to control the stereochemistry of 4-HIL. In this study, we modified the key enzyme 4-HIL dehydrogenase (HILDH) to overcome the biggest limitation in commercial-scale production of 4-HIL. As a result, an effective one-step carbonyl reduction to produce (2S,3R,4S)-4-HIL was successfully accomplished with strict stereoselectivity (>99% de). Mass production of (2S,3R,4S)-4-HIL by our synthetic method could have a significant contribution to the prevention of diabetes, dyslipidemia, and Alzheimer's disease. (120 words/200 words).
		+
		+	Engineering a short-chain dehydrogenase/reductase for the stereoselective production of (2S,3R,4S)-4-hydroxyisoleucine with three asymmetric centers.,Shi X, Miyakawa T, Nakamura A, Hou F, Hibi M, Ogawa J, Kwon Y, Tanokura M Sci Rep. 2017 Oct 20;7(1):13703. doi: 10.1038/s41598-017-13978-w. PMID:29057974<ref>PMID:29057974</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5gwr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus cereus var. thuringiensis smith et al. 1952]]
	[[Category: Miyakawa, T]]		[[Category: Miyakawa, T]]
	[[Category: Nakamura, A]]		[[Category: Nakamura, A]]

OCA at 07:09, 25 October 2017

OCA — Wed, 25 Oct 2017 07:09:51 GMT

←Older revision		Revision as of 07:09, 25 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5gwr is ~~ON HOLD until Paper Publication~~	+	==4-hydroxyisoleucine dehydrogenase complexed with NADH==
-		+	<StructureSection load='5gwr' size='340' side='right' caption='[[5gwr]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~Authors~~: ~~Shi~~, X., ~~Miyakawa~~, T., ~~Nakamura~~, A., ~~Tanokura, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5gwr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GWR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GWR FirstGlance]. <br>
-	~~Description~~: ~~4-hydroxyisoleucine dehydrogenase complexed with NADH~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gws\|5gws]], [[5gwt\|5gwt]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gwr OCA], [http://pdbe.org/5gwr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gwr RCSB], [http://www.ebi.ac.uk/pdbsum/5gwr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gwr ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Miyakawa, T]]
		+	[[Category: Nakamura, A]]
	[[Category: Shi, X]]		[[Category: Shi, X]]
	[[Category: Tanokura, M]]		[[Category: Tanokura, M]]
-	[[Category: ~~Miyakawa, T~~]]	+	[[Category: Dehydrogenase]]
-	[[Category: ~~Nakamura, A~~]]	+	[[Category: Nadh-dependent]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Reductase]]
		+	[[Category: Short-chain]]

OCA at 11:30, 3 October 2016

OCA — Mon, 03 Oct 2016 11:30:51 GMT

←Older revision		Revision as of 11:30, 3 October 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5gwr is ON HOLD	+	The entry 5gwr is ON HOLD until Paper Publication

	Authors: Shi, X., Miyakawa, T., Nakamura, A., Tanokura, M.		Authors: Shi, X., Miyakawa, T., Nakamura, A., Tanokura, M.

OCA: Protected "5gwr" [edit=sysop:move=sysop]

OCA — Wed, 21 Sep 2016 08:43:58 GMT

Protected "5gwr" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:43, 21 September 2016

OCA: New page: '''Unreleased structure''' The entry 5gwr is ON HOLD Authors: Shi, X., Miyakawa, T., Nakamura, A., Tanokura, M. Description: 4-hydroxyisoleucine dehydrogenase complexed with NADH [[Cat...

OCA — Wed, 21 Sep 2016 08:43:57 GMT

New page: '''Unreleased structure''' The entry 5gwr is ON HOLD Authors: Shi, X., Miyakawa, T., Nakamura, A., Tanokura, M. Description: 4-hydroxyisoleucine dehydrogenase complexed with NADH [[Cat...

New page

'''Unreleased structure'''

The entry 5gwr is ON HOLD

Authors: Shi, X., Miyakawa, T., Nakamura, A., Tanokura, M.

Description: 4-hydroxyisoleucine dehydrogenase complexed with NADH
[[Category: Unreleased Structures]]
[[Category: Shi, X]]
[[Category: Tanokura, M]]
[[Category: Miyakawa, T]]
[[Category: Nakamura, A]]