5gy3 - Revision history

OCA at 09:14, 20 March 2024

OCA — Wed, 20 Mar 2024 09:14:11 GMT

←Older revision		Revision as of 09:14, 20 March 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A0J4VP90_KLEVA A0A0J4VP90_KLEVA]		[https://www.uniprot.org/uniprot/A0A0J4VP90_KLEVA A0A0J4VP90_KLEVA]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Cellulases are produced by microorganisms that grow on cellulose biomass. Here, a cellulase, Cel10, was identified in a strain of Klebsiella pneumoniae isolated from Chinese bamboo rat gut. Analysis of substrate specificity showed that Cel10 is able to hydrolyze amorphous carboxymethyl cellulose (CMC) and crystalline forms of cellulose (Avicel and xylan) but is unable to hydrolyze p-nitrophenol beta-D-glucopyranoside (p-NPG), proving that Cel10 is an endoglucanase. A phylogenetic tree analysis indicates that Cel10 belongs to the glycoside hydrolase 8 (GH8) subfamily. In order to further understanding of its substrate specificity, the structure of Cel10 was solved by molecular replacement and refined to 1.76 A resolution. The overall fold is distinct from those of most other enzymes belonging to the GH8 subfamily. Although it forms the typical (alpha/alpha)6-barrel motif fold, like Acetobacterxylinum CMCax, one helix is missing. Structural comparisons with Clostridium thermocellum CelA (CtCelA), the best characterized GH8 endoglucanase, revealed that sugar-recognition subsite -3 is completely missing in Cel10. The absence of this subsite correlates to a more open substrate-binding cleft on the cellooligosaccharide reducing-end side.
-
-	The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae.,Attigani A, Sun L, Wang Q, Liu Y, Bai D, Li S, Huang X Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):870-876. Epub 2016, Nov 25. PMID:27917834<ref>PMID:27917834</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5gy3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:45, 2 August 2023

OCA — Wed, 02 Aug 2023 11:45:51 GMT

←Older revision		Revision as of 11:45, 2 August 2023
Line 3:		Line 3:
	<StructureSection load='5gy3' size='340' side='right'caption='[[5gy3]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='5gy3' size='340' side='right'caption='[[5gy3]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gy3]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus~~ pneumoniae~~" (schroeter 1886) flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GY3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GY3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gy3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GY3 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>cel10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~573~~ "~~Bacillus pneumoniae~~" ~~(Schroeter 1886) Flugge 1886~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.77Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5gy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gy3 OCA], [~~http~~://pdbe.org/5gy3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gy3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gy3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gy3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gy3 OCA], [https://pdbe.org/5gy3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gy3 RCSB], [https://www.ebi.ac.uk/pdbsum/5gy3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gy3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0J4VP90_KLEVA A0A0J4VP90_KLEVA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klebsiella pneumoniae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Attigani, A]]	+	[[Category: Attigani A]]
-	[[Category: Li~~, S P~~]]	+	[[Category: Li SP]]
-	[[Category: Sun~~, L F]]~~	+	[[Category: Sun LF]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:46, 20 May 2020

OCA — Wed, 20 May 2020 06:46:01 GMT

←Older revision		Revision as of 06:46, 20 May 2020
Line 1:		Line 1:

	==The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae==		==The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae==
-	<StructureSection load='5gy3' size='340' side='right' caption='[[5gy3]], [[Resolution\|resolution]] 1.77Å' scene=''>	+	<StructureSection load='5gy3' size='340' side='right'caption='[[5gy3]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gy3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GY3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GY3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gy3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GY3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GY3 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gy3 OCA], [http://pdbe.org/5gy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gy3 RCSB], [http://www.ebi.ac.uk/pdbsum/5gy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gy3 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cel10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gy3 OCA], [http://pdbe.org/5gy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gy3 RCSB], [http://www.ebi.ac.uk/pdbsum/5gy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gy3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 5gy3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5gy3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Attigani, A]]		[[Category: Attigani, A]]
	[[Category: Li, S P]]		[[Category: Li, S P]]
	[[Category: Sun, L F]]		[[Category: Sun, L F]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 21:24, 22 December 2016

OCA — Thu, 22 Dec 2016 21:24:02 GMT

←Older revision		Revision as of 21:24, 22 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5gy3 is ~~ON HOLD~~	+	==The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae==
		+	<StructureSection load='5gy3' size='340' side='right' caption='[[5gy3]], [[Resolution\|resolution]] 1.77Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5gy3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GY3 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gy3 OCA], [http://pdbe.org/5gy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gy3 RCSB], [http://www.ebi.ac.uk/pdbsum/5gy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gy3 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cellulases are produced by microorganisms that grow on cellulose biomass. Here, a cellulase, Cel10, was identified in a strain of Klebsiella pneumoniae isolated from Chinese bamboo rat gut. Analysis of substrate specificity showed that Cel10 is able to hydrolyze amorphous carboxymethyl cellulose (CMC) and crystalline forms of cellulose (Avicel and xylan) but is unable to hydrolyze p-nitrophenol beta-D-glucopyranoside (p-NPG), proving that Cel10 is an endoglucanase. A phylogenetic tree analysis indicates that Cel10 belongs to the glycoside hydrolase 8 (GH8) subfamily. In order to further understanding of its substrate specificity, the structure of Cel10 was solved by molecular replacement and refined to 1.76 A resolution. The overall fold is distinct from those of most other enzymes belonging to the GH8 subfamily. Although it forms the typical (alpha/alpha)6-barrel motif fold, like Acetobacterxylinum CMCax, one helix is missing. Structural comparisons with Clostridium thermocellum CelA (CtCelA), the best characterized GH8 endoglucanase, revealed that sugar-recognition subsite -3 is completely missing in Cel10. The absence of this subsite correlates to a more open substrate-binding cleft on the cellooligosaccharide reducing-end side.

-	~~Authors: Attigani~~, A., Li, S.P., ~~Sun, L.F~~.	+	The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae.,Attigani A, Sun L, Wang Q, Liu Y, Bai D, Li S, Huang X Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):870-876. Epub 2016, Nov 25. PMID:27917834<ref>PMID:27917834</ref>

-	~~Description: The crystal structure of endoglucanase Cel10~~, a ~~family 8 glycosyl hydrolase from Klebsiella pneumoniae~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5gy3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Attigani, A]]		[[Category: Attigani, A]]
-	[[Category: Sun, L.F]]	+	[[Category: Li, S P]]
-	[[Category: ~~Li, S.P~~]]	+	[[Category: Sun, L F]]
		+	[[Category: Hydrolase]]

OCA: Protected "5gy3" [edit=sysop:move=sysop]

OCA — Mon, 03 Oct 2016 11:33:46 GMT

Protected "5gy3" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:33, 3 October 2016

OCA: New page: '''Unreleased structure''' The entry 5gy3 is ON HOLD Authors: Attigani, A., Li, S.P., Sun, L.F. Description: The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase...

OCA — Mon, 03 Oct 2016 11:33:45 GMT

New page: '''Unreleased structure''' The entry 5gy3 is ON HOLD Authors: Attigani, A., Li, S.P., Sun, L.F. Description: The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase...

New page

'''Unreleased structure'''

The entry 5gy3 is ON HOLD

Authors: Attigani, A., Li, S.P., Sun, L.F.

Description: The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
[[Category: Unreleased Structures]]
[[Category: Attigani, A]]
[[Category: Sun, L.F]]
[[Category: Li, S.P]]