5gye - Revision history

OCA at 11:46, 2 August 2023

OCA — Wed, 02 Aug 2023 11:46:07 GMT

←Older revision		Revision as of 11:46, 2 August 2023
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	<StructureSection load='5gye' size='340' side='right'caption='[[5gye]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5gye' size='340' side='right'caption='[[5gye]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gye]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5GYE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gye]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GYE FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5gv1\|5gv1]], [[5gy8\|5gy8]], [[5gy9\|5gy9]], [[5gya\|5gya]], [[5gyb\|5gyb]], [[5gyc\|5gyc]], [[5gyf\|5gyf]], [[5gyg\|5gyg]], [[5gyh\|5gyh]], [[5gyi\|5gyi]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo~~-1,~~4-beta-xylanase Endo-1,4-beta-xylanase~~]~~, with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.~~1.~~8 3.2.1.8] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gye OCA], [https://pdbe.org/5gye PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gye RCSB], [https://www.ebi.ac.uk/pdbsum/5gye PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gye ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5gye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gye OCA], [~~http~~://pdbe.org/5gye PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gye RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gye PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gye ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Termite gut metagenome]]		[[Category: Termite gut metagenome]]
-	[[Category: Boonyapakorn, K]]	+	[[Category: Boonyapakorn K]]
-	[[Category: Chitnumsub, P]]	+	[[Category: Chitnumsub P]]
-	[[Category: Jaruwat, A]]	+	[[Category: Jaruwat A]]
-	~~[[Category: 4-beta-xylanase]]~~	+
-	~~[[Category: Endo-1]]~~	+
-	~~[[Category: Gh11 xylanase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:46, 20 May 2020

OCA — Wed, 20 May 2020 06:46:19 GMT

←Older revision		Revision as of 06:46, 20 May 2020
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	==Crystal structure of ENZbleach xylanase T28C+T60C and T77C+E249C mutant==		==Crystal structure of ENZbleach xylanase T28C+T60C and T77C+E249C mutant==
-	<StructureSection load='5gye' size='340' side='right' caption='[[5gye]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5gye' size='340' side='right'caption='[[5gye]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gye]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYE OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GYE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gye]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GYE FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gv1\|5gv1]], [[5gy8\|5gy8]], [[5gy9\|5gy9]], [[5gya\|5gya]], [[5gyb\|5gyb]], [[5gyc\|5gyc]], [[5gyf\|5gyf]], [[5gyg\|5gyg]], [[5gyh\|5gyh]], [[5gyi\|5gyi]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gv1\|5gv1]], [[5gy8\|5gy8]], [[5gy9\|5gy9]], [[5gya\|5gya]], [[5gyb\|5gyb]], [[5gyc\|5gyc]], [[5gyf\|5gyf]], [[5gyg\|5gyg]], [[5gyh\|5gyh]], [[5gyi\|5gyi]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gye OCA], [http://pdbe.org/5gye PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gye RCSB], [http://www.ebi.ac.uk/pdbsum/5gye PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gye ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gye OCA], [http://pdbe.org/5gye PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gye RCSB], [http://www.ebi.ac.uk/pdbsum/5gye PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gye ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Large Structures]]
	[[Category: Termite gut metagenome]]		[[Category: Termite gut metagenome]]
	[[Category: Boonyapakorn, K]]		[[Category: Boonyapakorn, K]]

OCA at 17:18, 15 November 2017

OCA — Wed, 15 Nov 2017 17:18:56 GMT

←Older revision		Revision as of 17:18, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5gye' size='340' side='right' caption='[[5gye]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5gye' size='340' side='right' caption='[[5gye]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gye]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GYE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gye]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GYE FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gv1\|5gv1]], [[5gy8\|5gy8]], [[5gy9\|5gy9]], [[5gya\|5gya]], [[5gyb\|5gyb]], [[5gyc\|5gyc]], [[5gyf\|5gyf]], [[5gyg\|5gyg]], [[5gyh\|5gyh]], [[5gyi\|5gyi]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gv1\|5gv1]], [[5gy8\|5gy8]], [[5gy9\|5gy9]], [[5gya\|5gya]], [[5gyb\|5gyb]], [[5gyc\|5gyc]], [[5gyf\|5gyf]], [[5gyg\|5gyg]], [[5gyh\|5gyh]], [[5gyi\|5gyi]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Termite gut metagenome]]
	[[Category: Boonyapakorn, K]]		[[Category: Boonyapakorn, K]]
	[[Category: Chitnumsub, P]]		[[Category: Chitnumsub, P]]

OCA at 04:07, 30 August 2017

OCA — Wed, 30 Aug 2017 04:07:08 GMT

←Older revision		Revision as of 04:07, 30 August 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5gye is ~~ON HOLD~~	+	==Crystal structure of ENZbleach xylanase T28C+T60C and T77C+E249C mutant==
		+	<StructureSection load='5gye' size='340' side='right' caption='[[5gye]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5gye]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GYE FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gv1\|5gv1]], [[5gy8\|5gy8]], [[5gy9\|5gy9]], [[5gya\|5gya]], [[5gyb\|5gyb]], [[5gyc\|5gyc]], [[5gyf\|5gyf]], [[5gyg\|5gyg]], [[5gyh\|5gyh]], [[5gyi\|5gyi]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gye OCA], [http://pdbe.org/5gye PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gye RCSB], [http://www.ebi.ac.uk/pdbsum/5gye PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gye ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the pulp bleaching industry, enzymes with robust activity at high pH and temperatures are desirable for facilitating the pre-bleaching process with simplified processing and minimal use of chlorinated compounds. To engineer an enzyme for this purpose, we determined the crystal structure of the Xyn12.2 xylanase, a xylan-hydrolyzing enzyme derived from the termite gut symbiont metagenome, as the basis for structure-based protein engineering to improve Xyn12.2 stability in high heat and alkaline conditions. Engineered cysteine pairs that generated exterior disulfide bonds increased the kcat of Xyn12.2 variants and melting temperature at all tested conditions. These improvements led to up to 4.2-fold increases in catalytic efficiency at pH 9.0, 50 degrees C for 1h and up to 3-fold increases at 60 degrees C. The most effective variants, XynTT and XynTTTE, exhibited 2-3-fold increases in bagasse hydrolysis at pH 9.0 and 60 degrees C compared to the wild-type enzyme. Overall, engineering arginines and phenylalanines for increased pKa and hydrogen bonding improved enzyme catalytic efficiency at high stringency conditions. These modifications were the keys to enhancing thermostability and alkaliphilicity in our enzyme variants, with XynTT and XynTTTE being especially promising for their application to the pulp and paper industry.

-	~~Authors: Chitnumsub~~, P., ~~Jaruwat~~, A., ~~Boonyapakorn~~, K.	+	Structure-based protein engineering for thermostable and alkaliphilic enhancement of endo-beta-1,4-xylanase for applications in pulp bleaching.,Boonyapakron K, Jaruwat A, Liwnaree B, Nimchua T, Champreda V, Chitnumsub P J Biotechnol. 2017 Jul 31. pii: S0168-1656(17)31572-9. doi:, 10.1016/j.jbiotec.2017.07.035. PMID:28774672<ref>PMID:28774672</ref>

-	~~Description: Crystal structure~~ of ~~ENZbleach xylanase T28C+T60C and T77C+E249C mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Jaruwat, A]]~~	+	<div class="pdbe-citations 5gye" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Chitnumsub~~, P]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Boonyapakorn, K]]		[[Category: Boonyapakorn, K]]
		+	[[Category: Chitnumsub, P]]
		+	[[Category: Jaruwat, A]]
		+	[[Category: 4-beta-xylanase]]
		+	[[Category: Endo-1]]
		+	[[Category: Gh11 xylanase]]
		+	[[Category: Hydrolase]]

OCA: Protected "5gye" [edit=sysop:move=sysop]

OCA — Mon, 03 Oct 2016 11:34:42 GMT

Protected "5gye" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:34, 3 October 2016

OCA: New page: '''Unreleased structure''' The entry 5gye is ON HOLD Authors: Chitnumsub, P., Jaruwat, A., Boonyapakorn, K. Description: Crystal structure of ENZbleach xylanase T28C+T60C and T77C+E249...

OCA — Mon, 03 Oct 2016 11:34:41 GMT

New page: '''Unreleased structure''' The entry 5gye is ON HOLD Authors: Chitnumsub, P., Jaruwat, A., Boonyapakorn, K. Description: Crystal structure of ENZbleach xylanase T28C+T60C and T77C+E249...

New page

'''Unreleased structure'''

The entry 5gye is ON HOLD

Authors: Chitnumsub, P., Jaruwat, A., Boonyapakorn, K.

Description: Crystal structure of ENZbleach xylanase T28C+T60C and T77C+E249C mutant
[[Category: Unreleased Structures]]
[[Category: Jaruwat, A]]
[[Category: Chitnumsub, P]]
[[Category: Boonyapakorn, K]]