5gyg - Revision history

OCA at 11:46, 2 August 2023

OCA — Wed, 02 Aug 2023 11:46:08 GMT

←Older revision		Revision as of 11:46, 2 August 2023
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	<StructureSection load='5gyg' size='340' side='right'caption='[[5gyg]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='5gyg' size='340' side='right'caption='[[5gyg]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5gyg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5gyg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GYG FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5gv1\|5gv1]], [[5gy8\|5gy8]], [[5gy9\|5gy9]], [[5gya\|5gya]], [[5gyb\|5gyb]], [[5gyc\|5gyc]], [[5gye\|5gye]], [[5gyf\|5gyf]], [[5gyh\|5gyh]], [[5gyi\|5gyi]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.74Å</td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo~~-1,~~4-beta-xylanase Endo-1,4-beta-xylanase~~]~~, with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.~~1.~~8 3.2.1.8] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gyg OCA], [https://pdbe.org/5gyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gyg RCSB], [https://www.ebi.ac.uk/pdbsum/5gyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gyg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5gyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gyg OCA], [~~http~~://pdbe.org/5gyg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5gyg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5gyg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5gyg ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Termite gut metagenome]]		[[Category: Termite gut metagenome]]
-	[[Category: Boonyapakorn, K]]	+	[[Category: Boonyapakorn K]]
-	[[Category: Chitnumsub, P]]	+	[[Category: Chitnumsub P]]
-	[[Category: Jaruwat, A]]	+	[[Category: Jaruwat A]]
-	~~[[Category: 4-beta-xylanase]]~~	+
-	~~[[Category: Endo-1]]~~	+
-	~~[[Category: Gh11 xylanase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:39, 10 April 2019

OCA — Wed, 10 Apr 2019 07:39:30 GMT

←Older revision		Revision as of 07:39, 10 April 2019
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	==Crystal structure of ENZbleach xylanase T28C+T60C mutant with three N-teminal residue truncation==		==Crystal structure of ENZbleach xylanase T28C+T60C mutant with three N-teminal residue truncation==
-	<StructureSection load='5gyg' size='340' side='right' caption='[[5gyg]], [[Resolution\|resolution]] 1.74Å' scene=''>	+	<StructureSection load='5gyg' size='340' side='right'caption='[[5gyg]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5gyg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GYG FirstGlance]. <br>		<table><tr><td colspan='2'>[[5gyg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GYG FirstGlance]. <br>
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gyg OCA], [http://pdbe.org/5gyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gyg RCSB], [http://www.ebi.ac.uk/pdbsum/5gyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gyg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gyg OCA], [http://pdbe.org/5gyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gyg RCSB], [http://www.ebi.ac.uk/pdbsum/5gyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gyg ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the pulp bleaching industry, enzymes with robust activity at high pH and temperatures are desirable for facilitating the pre-bleaching process with simplified processing and minimal use of chlorinated compounds. To engineer an enzyme for this purpose, we determined the crystal structure of the Xyn12.2 xylanase, a xylan-hydrolyzing enzyme derived from the termite gut symbiont metagenome, as the basis for structure-based protein engineering to improve Xyn12.2 stability in high heat and alkaline conditions. Engineered cysteine pairs that generated exterior disulfide bonds increased the kcat of Xyn12.2 variants and melting temperature at all tested conditions. These improvements led to up to 4.2-fold increases in catalytic efficiency at pH 9.0, 50 degrees C for 1h and up to 3-fold increases at 60 degrees C. The most effective variants, XynTT and XynTTTE, exhibited 2-3-fold increases in bagasse hydrolysis at pH 9.0 and 60 degrees C compared to the wild-type enzyme. Overall, engineering arginines and phenylalanines for increased pKa and hydrogen bonding improved enzyme catalytic efficiency at high stringency conditions. These modifications were the keys to enhancing thermostability and alkaliphilicity in our enzyme variants, with XynTT and XynTTTE being especially promising for their application to the pulp and paper industry.
		+
		+	Structure-based protein engineering for thermostable and alkaliphilic enhancement of endo-beta-1,4-xylanase for applications in pulp bleaching.,Boonyapakron K, Jaruwat A, Liwnaree B, Nimchua T, Champreda V, Chitnumsub P J Biotechnol. 2017 Jul 31. pii: S0168-1656(17)31572-9. doi:, 10.1016/j.jbiotec.2017.07.035. PMID:28774672<ref>PMID:28774672</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5gyg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Large Structures]]
	[[Category: Termite gut metagenome]]		[[Category: Termite gut metagenome]]
	[[Category: Boonyapakorn, K]]		[[Category: Boonyapakorn, K]]

OCA at 11:44, 6 November 2017

OCA — Mon, 06 Nov 2017 11:44:08 GMT

←Older revision		Revision as of 11:44, 6 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5gyg is ~~ON HOLD~~	+	==Crystal structure of ENZbleach xylanase T28C+T60C mutant with three N-teminal residue truncation==
-		+	<StructureSection load='5gyg' size='340' side='right' caption='[[5gyg]], [[Resolution\|resolution]] 1.74Å' scene=''>
-	~~Authors~~: ~~Chitnumsub~~, P., ~~Jaruwat~~, A., ~~Boonyapakorn~~, K.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5gyg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Termite_gut_metagenome Termite gut metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GYG FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of ENZbleach xylanase T28C+T60C mutant with three N~~-~~teminal residue truncation~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5gv1\|5gv1]], [[5gy8\|5gy8]], [[5gy9\|5gy9]], [[5gya\|5gya]], [[5gyb\|5gyb]], [[5gyc\|5gyc]], [[5gye\|5gye]], [[5gyf\|5gyf]], [[5gyh\|5gyh]], [[5gyi\|5gyi]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gyg OCA], [http://pdbe.org/5gyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gyg RCSB], [http://www.ebi.ac.uk/pdbsum/5gyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gyg ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Termite gut metagenome]]
		+	[[Category: Boonyapakorn, K]]
		+	[[Category: Chitnumsub, P]]
	[[Category: Jaruwat, A]]		[[Category: Jaruwat, A]]
-	[[Category: ~~Chitnumsub, P~~]]	+	[[Category: 4-beta-xylanase]]
-	[[Category: ~~Boonyapakorn, K~~]]	+	[[Category: Endo-1]]
		+	[[Category: Gh11 xylanase]]
		+	[[Category: Hydrolase]]

OCA: Protected "5gyg" [edit=sysop:move=sysop]

OCA — Mon, 03 Oct 2016 11:34:49 GMT

Protected "5gyg" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:34, 3 October 2016

OCA: New page: '''Unreleased structure''' The entry 5gyg is ON HOLD Authors: Chitnumsub, P., Jaruwat, A., Boonyapakorn, K. Description: Crystal structure of ENZbleach xylanase T28C+T60C mutant with t...

OCA — Mon, 03 Oct 2016 11:34:47 GMT

New page: '''Unreleased structure''' The entry 5gyg is ON HOLD Authors: Chitnumsub, P., Jaruwat, A., Boonyapakorn, K. Description: Crystal structure of ENZbleach xylanase T28C+T60C mutant with t...

New page

'''Unreleased structure'''

The entry 5gyg is ON HOLD

Authors: Chitnumsub, P., Jaruwat, A., Boonyapakorn, K.

Description: Crystal structure of ENZbleach xylanase T28C+T60C mutant with three N-teminal residue truncation
[[Category: Unreleased Structures]]
[[Category: Jaruwat, A]]
[[Category: Chitnumsub, P]]
[[Category: Boonyapakorn, K]]