5h1x - Revision history

OCA at 07:21, 9 August 2023

OCA — Wed, 09 Aug 2023 07:21:17 GMT

←Older revision		Revision as of 07:21, 9 August 2023
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	<StructureSection load='5h1x' size='340' side='right'caption='[[5h1x]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='5h1x' size='340' side='right'caption='[[5h1x]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5h1x]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5H1X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5h1x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H1X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5h1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1x OCA], [~~http~~://pdbe.org/5h1x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5h1x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5h1x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.41Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1x OCA], [https://pdbe.org/5h1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h1x RCSB], [https://www.ebi.ac.uk/pdbsum/5h1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NUP62_RAT NUP62_RAT]] Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.	+	[https://www.uniprot.org/uniprot/NUP62_RAT NUP62_RAT] Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pravin, D~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Coiled-coil]]~~	+	[[Category: Pravin D]]
-	~~[[Category: Structural protein~~]]	+

OCA at 06:49, 20 May 2020

OCA — Wed, 20 May 2020 06:49:42 GMT

←Older revision		Revision as of 06:49, 20 May 2020
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	==Crystal Structure of rat Nup62 Coiled-coil motif==		==Crystal Structure of rat Nup62 Coiled-coil motif==
-	<StructureSection load='5h1x' size='340' side='right' caption='[[5h1x]], [[Resolution\|resolution]] 2.41Å' scene=''>	+	<StructureSection load='5h1x' size='340' side='right'caption='[[5h1x]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5h1x]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1X OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5H1X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5h1x]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H1X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5h1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1x OCA], [http://pdbe.org/5h1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h1x RCSB], [http://www.ebi.ac.uk/pdbsum/5h1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1x ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1x OCA], [http://pdbe.org/5h1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h1x RCSB], [http://www.ebi.ac.uk/pdbsum/5h1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5h1x" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5h1x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nucleoporin 3D structures\|Nucleoporin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pravin, D]]		[[Category: Pravin, D]]
	[[Category: Coiled-coil]]		[[Category: Coiled-coil]]
	[[Category: Structural protein]]		[[Category: Structural protein]]

OCA at 09:14, 4 October 2017

OCA — Wed, 04 Oct 2017 09:14:12 GMT

←Older revision		Revision as of 09:14, 4 October 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5h1x is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of rat Nup62 Coiled-coil motif==
		+	<StructureSection load='5h1x' size='340' side='right' caption='[[5h1x]], [[Resolution\|resolution]] 2.41Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5h1x]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H1X FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h1x OCA], [http://pdbe.org/5h1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h1x RCSB], [http://www.ebi.ac.uk/pdbsum/5h1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h1x ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NUP62_RAT NUP62_RAT]] Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The central transport channel of the vertebrate nuclear pore complex (NPC) consists of nucleoporins: Nup62, Nup54, and Nup58. The coiled-coil domains in alpha-helical regions of these nucleoporins are thought to be crucial for several protein-protein interactions in the NPC subcomplexes. In this study, we determined the crystal structure of the coiled-coil domain of rat Nup62 fragment (residues 362-425) to 2.4 A resolution. The crystal structure shows the conserved coiled-coil domain as a parallel three-helix bundle for the Nup62(362-425) fragment. On the basis of our size exclusion chromatography coupled to multiangle light scattering analysis and glutaraldehyde cross-linking experiments, we conclude that the Nup62(362-425) fragment displays dynamic behavior in solution and can also exist in either homodimeric or homotrimeric states. Our comparative analysis of the rat Nup62(362-425) homotrimeric structure with previously reported heterotrimeric structures [rat Nup62(362-425).Nup54(346-407) and Xenopus Nup62(358-485).Nup54(315-450).Nup58(283-406) complexes] demonstrates the structural basis for parallel triple-helix bundle formation for Nup62 with different partners. Moreover, we show that the coiled-coil domain of Nup62 is sufficient for interaction with the coiled-coil domain of rat Exo70, a protein in an exocyst complex. On the basis of these observations, we suggest the plausible chain replacement mechanism that yields to diverse protein assemblies with Nup62. In summary, the coiled-coil motif present in Nup62 imparts the ability to form a homotrimer and heterotrimers either with Nup54 or with Nup54-Nup58 within the NPCs as well as with Exo70 beyond the NPCs. These complexes of Nup62 suggest the crucial role of the coiled-coil motifs in providing plasticity to various modular assemblies.

-	~~Authors~~:	+	The Nup62 Coiled-Coil Motif Provides Plasticity for Triple-Helix Bundle Formation.,Dewangan PS, Sonawane PJ, Chouksey AR, Chauhan R Biochemistry. 2017 Jun 6;56(22):2803-2811. doi: 10.1021/acs.biochem.6b01050. Epub, 2017 Apr 26. PMID:28406021<ref>PMID:28406021</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5h1x" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Pravin, D]]
		+	[[Category: Coiled-coil]]
		+	[[Category: Structural protein]]

OCA at 13:38, 9 December 2016

OCA — Fri, 09 Dec 2016 13:38:57 GMT

←Older revision		Revision as of 13:38, 9 December 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5h1x is ON HOLD	+	The entry 5h1x is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5h1x" [edit=sysop:move=sysop]

OCA — Wed, 19 Oct 2016 12:03:22 GMT

Protected "5h1x" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:03, 19 October 2016

OCA: New page: '''Unreleased structure''' The entry 5h1x is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 Oct 2016 12:03:20 GMT

New page: '''Unreleased structure''' The entry 5h1x is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5h1x is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]