5h3a - Revision history

OCA at 07:22, 9 August 2023

OCA — Wed, 09 Aug 2023 07:22:30 GMT

←Older revision		Revision as of 07:22, 9 August 2023
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	<StructureSection load='5h3a' size='340' side='right'caption='[[5h3a]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5h3a' size='340' side='right'caption='[[5h3a]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5h3a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hhv-8 Hhv-~~8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H3A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5H3A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5h3a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_gammaherpesvirus_8 Human gammaherpesvirus 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H3A FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=D16:TOMUDEX'>D16</scene>~~, ~~<scene name='pdbligand=UMP:~~2~~-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5h38\|5h38]], [[5h39\|5h39]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D16:TOMUDEX'>D16</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class~~=~~"sblockDat">ORF70 ([http~~:~~//www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=~~5~~&id=37296 HHV~~-~~8])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h3a OCA], [https://pdbe.org/5h3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h3a RCSB], [https://www.ebi.ac.uk/pdbsum/5h3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h3a ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5h3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h3a OCA], [~~http~~://pdbe.org/5h3a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5h3a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5h3a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5h3a ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TYSY_HHV8P TYSY_HHV8P]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5h3a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5h3a" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thymidylate synthase 3D structures\|Thymidylate synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hhv-~~8]]	+	[[Category: Human gammaherpesvirus 8]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Choi~~, Y M~~]]	+	[[Category: Choi YM]]
-	[[Category: Lee~~, J Y~~]]	+	[[Category: Lee JY]]
-	[[Category: Park~~, Y W~~]]	+	[[Category: Park YW]]
-	[[Category: Yeo~~, H K]]~~	+	[[Category: Yeo HK]]
-	~~[[Category: Dump]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Raltitrexed]]~~	+
-	~~[[Category: Thymidylate synthase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:51, 20 May 2020

OCA — Wed, 20 May 2020 06:51:17 GMT

←Older revision		Revision as of 06:51, 20 May 2020
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	==Structural analysis of KSHV thymidylate synthase==		==Structural analysis of KSHV thymidylate synthase==
-	<StructureSection load='5h3a' size='340' side='right' caption='[[5h3a]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5h3a' size='340' side='right'caption='[[5h3a]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5h3a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H3A OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5H3A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5h3a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hhv-8 Hhv-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H3A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H3A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=D16:TOMUDEX'>D16</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D16:TOMUDEX'>D16</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h38\|5h38]], [[5h39\|5h39]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h38\|5h38]], [[5h39\|5h39]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5h3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h3a OCA], [http://pdbe.org/5h3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h3a RCSB], [http://www.ebi.ac.uk/pdbsum/5h3a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h3a ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORF70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37296 HHV-8])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h3a OCA], [http://pdbe.org/5h3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h3a RCSB], [http://www.ebi.ac.uk/pdbsum/5h3a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h3a ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hhv-8]]
		+	[[Category: Large Structures]]
	[[Category: Choi, Y M]]		[[Category: Choi, Y M]]
	[[Category: Lee, J Y]]		[[Category: Lee, J Y]]

OCA at 16:16, 18 January 2017

OCA — Wed, 18 Jan 2017 16:16:09 GMT

←Older revision		Revision as of 16:16, 18 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5h3a is ~~ON HOLD until Paper~~ Publication	+	==Structural analysis of KSHV thymidylate synthase==
		+	<StructureSection load='5h3a' size='340' side='right' caption='[[5h3a]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5h3a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H3A FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=D16:TOMUDEX'>D16</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h38\|5h38]], [[5h39\|5h39]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h3a OCA], [http://pdbe.org/5h3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h3a RCSB], [http://www.ebi.ac.uk/pdbsum/5h3a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h3a ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kaposi's sarcoma-associated herpesvirus (KSHV) is a highly infectious human herpesvirus that causes Kaposi's sarcoma. KSHV encodes functional thymidylate synthase, which is a target for anticancer drugs such as raltitrexed or 5-fluorouracil. Thymidylate synthase catalyzes the conversion of 2'-deoxyuridine-5'-monophosphate (dUMP) to thymidine-5'-monophosphate (dTMP) using 5,10-methylenetetrahydrofolate (mTHF) as a co-substrate. The crystal structures of thymidylate synthase from KSHV (apo), complexes with dUMP (binary), and complexes with both dUMP and raltitrexed (ternary) were determined at 1.7 A, 2.0 A, and 2.4 A, respectively. While the ternary complex structures of human thymidylate synthase and E. coli thymidylate synthase had a closed conformation, the ternary complex structure of KSHV thymidylate synthase was observed in an open conformation, similar to that of rat thymidylate synthase. The complex structures of KSHV thymidylate synthase did not have a covalent bond between the sulfhydryl group of Cys219 and C6 atom of dUMP, unlike the human thymidylate synthase. The catalytic Cys residue demonstrated a dual conformation in the apo structure, and its sulfhydryl group was oriented toward the C6 atom of dUMP with no covalent bond upon ligand binding in the complex structures. These structural data provide the potential use of antifolates such as raltitrexed as a viral induced anticancer drug and structural basis to design drugs for targeting the thymidylate synthase of KSHV.

-	~~Authors: Choi, Y.M~~., Yeo~~, H.K.~~, Park, Y.W.~~, Lee, J~~.Y.	+	Structural Analysis of Thymidylate Synthase from Kaposi's Sarcoma-Associated Herpesvirus with the Anticancer Drug Raltitrexed.,Choi YM, Yeo HK, Park YW, Lee JY PLoS One. 2016 Dec 9;11(12):e0168019. doi: 10.1371/journal.pone.0168019., eCollection 2016. PMID:27936107<ref>PMID:27936107</ref>

-	~~Description: Structural analysis~~ of ~~KSHV thymidylate synthase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Park~~, Y.W]]	+	<div class="pdbe-citations 5h3a" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Choi~~, Y.M]]	+	== References ==
-	[[Category: Yeo, H.K]]	+	<references/>
-	[[Category: ~~Lee, J.Y~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Choi, Y M]]
		+	[[Category: Lee, J Y]]
		+	[[Category: Park, Y W]]
		+	[[Category: Yeo, H K]]
		+	[[Category: Dump]]
		+	[[Category: Inhibitor]]
		+	[[Category: Raltitrexed]]
		+	[[Category: Thymidylate synthase]]
		+	[[Category: Transferase]]

OCA at 13:41, 9 December 2016

OCA — Fri, 09 Dec 2016 13:41:23 GMT

←Older revision		Revision as of 13:41, 9 December 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5h3a is ON HOLD	+	The entry 5h3a is ON HOLD until Paper Publication

	Authors: Choi, Y.M., Yeo, H.K., Park, Y.W., Lee, J.Y.		Authors: Choi, Y.M., Yeo, H.K., Park, Y.W., Lee, J.Y.

OCA at 12:55, 2 November 2016

OCA — Wed, 02 Nov 2016 12:55:48 GMT

←Older revision		Revision as of 12:55, 2 November 2016
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	Authors: Choi, Y.M., Yeo, H.K., Park, Y.W., Lee, J.Y.		Authors: Choi, Y.M., Yeo, H.K., Park, Y.W., Lee, J.Y.

-	Description: KSHV thymidylate synthase	+	Description: Structural analysis of KSHV thymidylate synthase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Park, Y.W]]		[[Category: Park, Y.W]]

OCA: Protected "5h3a" [edit=sysop:move=sysop]

OCA — Wed, 26 Oct 2016 13:17:32 GMT

Protected "5h3a" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:17, 26 October 2016

OCA: New page: '''Unreleased structure''' The entry 5h3a is ON HOLD Authors: Choi, Y.M., Yeo, H.K., Park, Y.W., Lee, J.Y. Description: KSHV thymidylate synthase Category: Unreleased Structures [[...

OCA — Wed, 26 Oct 2016 13:17:31 GMT

New page: '''Unreleased structure''' The entry 5h3a is ON HOLD Authors: Choi, Y.M., Yeo, H.K., Park, Y.W., Lee, J.Y. Description: KSHV thymidylate synthase Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 5h3a is ON HOLD

Authors: Choi, Y.M., Yeo, H.K., Park, Y.W., Lee, J.Y.

Description: KSHV thymidylate synthase
[[Category: Unreleased Structures]]
[[Category: Park, Y.W]]
[[Category: Choi, Y.M]]
[[Category: Yeo, H.K]]
[[Category: Lee, J.Y]]