5h9v - Revision history

OCA at 07:28, 9 August 2023

OCA — Wed, 09 Aug 2023 07:28:22 GMT

←Older revision		Revision as of 07:28, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='5h9v' size='340' side='right'caption='[[5h9v]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='5h9v' size='340' side='right'caption='[[5h9v]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5h9v]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H9V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5H9V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5h9v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H9V FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat">[[5h9w\|5h9w]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h9v OCA], [https://pdbe.org/5h9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h9v RCSB], [https://www.ebi.ac.uk/pdbsum/5h9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h9v ProSAT]</span></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">Zc3h12a, Mcpip, Mcpip1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5h9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h9v OCA], [~~http~~://pdbe.org/5h9v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5h9v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5h9v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5h9v ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/ZC12A_MOUSE ZC12A_MOUSE]] Increased expression of ZC3H12A is associated with ischemic heart disease (PubMed:16574901).<ref>PMID:16574901</ref>	+	[https://www.uniprot.org/uniprot/ZC12A_MOUSE ZC12A_MOUSE] Increased expression of ZC3H12A is associated with ischemic heart disease (PubMed:16574901).<ref>PMID:16574901</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ZC12A_MOUSE ZC12A_MOUSE]] Bifunctional enzyme with both endoribonuclease and deubiquitinase activities involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay (PubMed:26000482). Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation (PubMed:19322177, PubMed:21115689, PubMed:23185455, PubMed:26000482). Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines (IL6 and IL2), cell surface receptors (ICOS, TNFRSF4 and TNFR2) and transcription factor (REL) (PubMed:23706741). Self regulates by destabilizing its own mRNA (PubMed:22037600). Cleaves mRNA harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-dependent manner (PubMed:19322177, PubMed:23185455, PubMed:23706741, PubMed:26000482, PubMed:26134560). Plays a role in the inhibition of microRNAs (miRNAs) biogenesis (By similarity). Cleaves the terminal loop of a set of precursor miRNAs (pre-miRNAs) important for the regulation of the inflammatory response leading to their degradation, and thus preventing the biosynthesis of mature miRNAs (By similarity). Plays also a role in promoting angiogenesis in response to inflammatory cytokines by inhibiting the production of antiangiogenic microRNAs via its anti-dicer RNase activity (By similarity). Functions as a deubiquitinase that affects the overall ubiquitination of cellular proteins (PubMed:21115689). Possesses deubiquitinase activity that specifically cleaves 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains on TNF receptor-associated factors (TRAFs), preventing JNK and NF-kappa-B signaling pathway activation, and hence negatively regulates macrophage-mediated inflammatory response and immune homeostasis (PubMed:21115689). Deubiquitinates also the transcription factor HIF1A, probably leading to its stabilization and nuclear import, thereby positively regulating the expression of proangiogenic HIF1A-targeted genes (By similarity). Prevents stress granules (SGs) formation and promotes macrophage apoptosis under stress conditions, including arsenite-induced oxidative stress, heat shock, and energy deprivation, which may be dependent on its deubiquitinase activity (PubMed:21971051). Plays a role in the regulation of macrophage polarization; promotes IL4-induced polarization of macrophages M1 into anti-inflammatory M2 state, depending on both endoribonuclease and deubiquitinase activities (PubMed:25934862). May also act as a transcription factor that regulates the expression of multiple genes involved in inflammatory response, angiogenesis, adipogenesis and apoptosis (PubMed:18178554, PubMed:19666473, PubMed:22739135). Functions as a positive regulator of glial differentiation of neuroprogenitor cells through an amyloid precursor protein (APP)-dependent signaling pathway (By similarity). Attenuates septic myocardial contractile dysfunction in response to lipopolysaccharide (LPS) by reducing I-kappa-B-kinase (IKK)-mediated NF-kappa-B activation, and hence myocardial proinflammatory cytokine production (PubMed:21616078).[UniProtKB:Q5D1E8]<ref>PMID:18178554</ref> <ref>PMID:19322177</ref> <ref>PMID:19666473</ref> <ref>PMID:21115689</ref> <ref>PMID:21616078</ref> <ref>PMID:21971051</ref> <ref>PMID:22037600</ref> <ref>PMID:22739135</ref> <ref>PMID:23185455</ref> <ref>PMID:23706741</ref> <ref>PMID:25934862</ref> <ref>PMID:26000482</ref> <ref>PMID:26134560</ref>	+	[https://www.uniprot.org/uniprot/ZC12A_MOUSE ZC12A_MOUSE] Bifunctional enzyme with both endoribonuclease and deubiquitinase activities involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay (PubMed:26000482). Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation (PubMed:19322177, PubMed:21115689, PubMed:23185455, PubMed:26000482). Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines (IL6 and IL2), cell surface receptors (ICOS, TNFRSF4 and TNFR2) and transcription factor (REL) (PubMed:23706741). Self regulates by destabilizing its own mRNA (PubMed:22037600). Cleaves mRNA harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-dependent manner (PubMed:19322177, PubMed:23185455, PubMed:23706741, PubMed:26000482, PubMed:26134560). Plays a role in the inhibition of microRNAs (miRNAs) biogenesis (By similarity). Cleaves the terminal loop of a set of precursor miRNAs (pre-miRNAs) important for the regulation of the inflammatory response leading to their degradation, and thus preventing the biosynthesis of mature miRNAs (By similarity). Plays also a role in promoting angiogenesis in response to inflammatory cytokines by inhibiting the production of antiangiogenic microRNAs via its anti-dicer RNase activity (By similarity). Functions as a deubiquitinase that affects the overall ubiquitination of cellular proteins (PubMed:21115689). Possesses deubiquitinase activity that specifically cleaves 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains on TNF receptor-associated factors (TRAFs), preventing JNK and NF-kappa-B signaling pathway activation, and hence negatively regulates macrophage-mediated inflammatory response and immune homeostasis (PubMed:21115689). Deubiquitinates also the transcription factor HIF1A, probably leading to its stabilization and nuclear import, thereby positively regulating the expression of proangiogenic HIF1A-targeted genes (By similarity). Prevents stress granules (SGs) formation and promotes macrophage apoptosis under stress conditions, including arsenite-induced oxidative stress, heat shock, and energy deprivation, which may be dependent on its deubiquitinase activity (PubMed:21971051). Plays a role in the regulation of macrophage polarization; promotes IL4-induced polarization of macrophages M1 into anti-inflammatory M2 state, depending on both endoribonuclease and deubiquitinase activities (PubMed:25934862). May also act as a transcription factor that regulates the expression of multiple genes involved in inflammatory response, angiogenesis, adipogenesis and apoptosis (PubMed:18178554, PubMed:19666473, PubMed:22739135). Functions as a positive regulator of glial differentiation of neuroprogenitor cells through an amyloid precursor protein (APP)-dependent signaling pathway (By similarity). Attenuates septic myocardial contractile dysfunction in response to lipopolysaccharide (LPS) by reducing I-kappa-B-kinase (IKK)-mediated NF-kappa-B activation, and hence myocardial proinflammatory cytokine production (PubMed:21616078).[UniProtKB:Q5D1E8]<ref>PMID:18178554</ref> <ref>PMID:19322177</ref> <ref>PMID:19666473</ref> <ref>PMID:21115689</ref> <ref>PMID:21616078</ref> <ref>PMID:21971051</ref> <ref>PMID:22037600</ref> <ref>PMID:22739135</ref> <ref>PMID:23185455</ref> <ref>PMID:23706741</ref> <ref>PMID:25934862</ref> <ref>PMID:26000482</ref> <ref>PMID:26134560</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 31:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Adachi, W]]	+	[[Category: Adachi W]]
-	[[Category: Inagaki, F]]	+	[[Category: Inagaki F]]
-	[[Category: Noda~~, N N~~]]	+	[[Category: Noda NN]]
-	[[Category: Tsushima, T]]	+	[[Category: Tsushima T]]
-	[[Category: Yokogawa, M]]	+	[[Category: Yokogawa M]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Rnase~~]]	+

OCA at 07:15, 19 February 2020

OCA — Wed, 19 Feb 2020 07:15:39 GMT

←Older revision		Revision as of 07:15, 19 February 2020
Line 1:		Line 1:

	==Crystal structure of Regnase PIN domain, form I==		==Crystal structure of Regnase PIN domain, form I==
-	<StructureSection load='5h9v' size='340' side='right' caption='[[5h9v]], [[Resolution\|resolution]] 2.75Å' scene=''>	+	<StructureSection load='5h9v' size='340' side='right'caption='[[5h9v]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5h9v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H9V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H9V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5h9v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H9V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H9V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h9w\|5h9w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h9w\|5h9w]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h9v OCA], [http://pdbe.org/5h9v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h9v RCSB], [http://www.ebi.ac.uk/pdbsum/5h9v PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Zc3h12a, Mcpip, Mcpip1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h9v OCA], [http://pdbe.org/5h9v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h9v RCSB], [http://www.ebi.ac.uk/pdbsum/5h9v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h9v ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 22:		Line 23:
	</div>		</div>
	<div class="pdbe-citations 5h9v" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5h9v" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Adachi, W]]		[[Category: Adachi, W]]
	[[Category: Inagaki, F]]		[[Category: Inagaki, F]]

OCA at 03:52, 11 May 2016

OCA — Wed, 11 May 2016 03:52:00 GMT

←Older revision		Revision as of 03:52, 11 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5h9v is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of Regnase PIN domain, form I==
		+	<StructureSection load='5h9v' size='340' side='right' caption='[[5h9v]], [[Resolution\|resolution]] 2.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5h9v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H9V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H9V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5h9w\|5h9w]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h9v OCA], [http://pdbe.org/5h9v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h9v RCSB], [http://www.ebi.ac.uk/pdbsum/5h9v PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/ZC12A_MOUSE ZC12A_MOUSE]] Increased expression of ZC3H12A is associated with ischemic heart disease (PubMed:16574901).<ref>PMID:16574901</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ZC12A_MOUSE ZC12A_MOUSE]] Bifunctional enzyme with both endoribonuclease and deubiquitinase activities involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay (PubMed:26000482). Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation (PubMed:19322177, PubMed:21115689, PubMed:23185455, PubMed:26000482). Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines (IL6 and IL2), cell surface receptors (ICOS, TNFRSF4 and TNFR2) and transcription factor (REL) (PubMed:23706741). Self regulates by destabilizing its own mRNA (PubMed:22037600). Cleaves mRNA harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-dependent manner (PubMed:19322177, PubMed:23185455, PubMed:23706741, PubMed:26000482, PubMed:26134560). Plays a role in the inhibition of microRNAs (miRNAs) biogenesis (By similarity). Cleaves the terminal loop of a set of precursor miRNAs (pre-miRNAs) important for the regulation of the inflammatory response leading to their degradation, and thus preventing the biosynthesis of mature miRNAs (By similarity). Plays also a role in promoting angiogenesis in response to inflammatory cytokines by inhibiting the production of antiangiogenic microRNAs via its anti-dicer RNase activity (By similarity). Functions as a deubiquitinase that affects the overall ubiquitination of cellular proteins (PubMed:21115689). Possesses deubiquitinase activity that specifically cleaves 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains on TNF receptor-associated factors (TRAFs), preventing JNK and NF-kappa-B signaling pathway activation, and hence negatively regulates macrophage-mediated inflammatory response and immune homeostasis (PubMed:21115689). Deubiquitinates also the transcription factor HIF1A, probably leading to its stabilization and nuclear import, thereby positively regulating the expression of proangiogenic HIF1A-targeted genes (By similarity). Prevents stress granules (SGs) formation and promotes macrophage apoptosis under stress conditions, including arsenite-induced oxidative stress, heat shock, and energy deprivation, which may be dependent on its deubiquitinase activity (PubMed:21971051). Plays a role in the regulation of macrophage polarization; promotes IL4-induced polarization of macrophages M1 into anti-inflammatory M2 state, depending on both endoribonuclease and deubiquitinase activities (PubMed:25934862). May also act as a transcription factor that regulates the expression of multiple genes involved in inflammatory response, angiogenesis, adipogenesis and apoptosis (PubMed:18178554, PubMed:19666473, PubMed:22739135). Functions as a positive regulator of glial differentiation of neuroprogenitor cells through an amyloid precursor protein (APP)-dependent signaling pathway (By similarity). Attenuates septic myocardial contractile dysfunction in response to lipopolysaccharide (LPS) by reducing I-kappa-B-kinase (IKK)-mediated NF-kappa-B activation, and hence myocardial proinflammatory cytokine production (PubMed:21616078).[UniProtKB:Q5D1E8]<ref>PMID:18178554</ref> <ref>PMID:19322177</ref> <ref>PMID:19666473</ref> <ref>PMID:21115689</ref> <ref>PMID:21616078</ref> <ref>PMID:21971051</ref> <ref>PMID:22037600</ref> <ref>PMID:22739135</ref> <ref>PMID:23185455</ref> <ref>PMID:23706741</ref> <ref>PMID:25934862</ref> <ref>PMID:26000482</ref> <ref>PMID:26134560</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Regnase-1 is an RNase that directly cleaves mRNAs of inflammatory genes such as IL-6 and IL-12p40, and negatively regulates cellular inflammatory responses. Here, we report the structures of four domains of Regnase-1 from Mus musculus-the N-terminal domain (NTD), PilT N-terminus like (PIN) domain, zinc finger (ZF) domain and C-terminal domain (CTD). The PIN domain harbors the RNase catalytic center; however, it is insufficient for enzymatic activity. We found that the NTD associates with the PIN domain and significantly enhances its RNase activity. The PIN domain forms a head-to-tail oligomer and the dimer interface overlaps with the NTD binding site. Interestingly, mutations blocking PIN oligomerization had no RNase activity, indicating that both oligomerization and NTD binding are crucial for RNase activity in vitro. These results suggest that Regnase-1 RNase activity is tightly controlled by both intramolecular (NTD-PIN) and intermolecular (PIN-PIN) interactions.

-	~~Authors: Yokogawa~~, M., Tsushima, T., ~~Adachi~~, W., ~~Noda~~, ~~N.N.~~, ~~Inagaki~~, F.	+	Structural basis for the regulation of enzymatic activity of Regnase-1 by domain-domain interactions.,Yokogawa M, Tsushima T, Noda NN, Kumeta H, Enokizono Y, Yamashita K, Standley DM, Takeuchi O, Akira S, Inagaki F Sci Rep. 2016 Mar 1;6:22324. doi: 10.1038/srep22324. PMID:26927947<ref>PMID:26927947</ref>

-	~~Description: Crystal structure of Regnase PIN domain~~, ~~form I~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5h9v" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Adachi, W]]		[[Category: Adachi, W]]
	[[Category: Inagaki, F]]		[[Category: Inagaki, F]]
-	[[Category: ~~Yokogawa~~, M]]	+	[[Category: Noda, N N]]
	[[Category: Tsushima, T]]		[[Category: Tsushima, T]]
-	[[Category: ~~Noda~~, ~~N.N~~]]	+	[[Category: Yokogawa, M]]
		+	[[Category: Hydrolase]]
		+	[[Category: Rnase]]

OCA at 18:42, 13 January 2016

OCA — Wed, 13 Jan 2016 18:42:17 GMT

←Older revision		Revision as of 18:42, 13 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5h9v is ON HOLD	+	The entry 5h9v is ON HOLD until Paper Publication

	Authors: Yokogawa, M., Tsushima, T., Adachi, W., Noda, N.N., Inagaki, F.		Authors: Yokogawa, M., Tsushima, T., Adachi, W., Noda, N.N., Inagaki, F.

-	Description: Crystal structure of Regnase PIN domain, form I.	+	Description: Crystal structure of Regnase PIN domain, form I
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Adachi, W]]		[[Category: Adachi, W]]

OCA: Protected "5h9v" [edit=sysop:move=sysop]

OCA — Thu, 07 Jan 2016 10:22:18 GMT

Protected "5h9v" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:22, 7 January 2016

OCA: New page: '''Unreleased structure''' The entry 5h9v is ON HOLD Authors: Yokogawa, M., Tsushima, T., Adachi, W., Noda, N.N., Inagaki, F. Description: Crystal structure of Regnase PIN domain, form...

OCA — Thu, 07 Jan 2016 10:22:16 GMT

New page: '''Unreleased structure''' The entry 5h9v is ON HOLD Authors: Yokogawa, M., Tsushima, T., Adachi, W., Noda, N.N., Inagaki, F. Description: Crystal structure of Regnase PIN domain, form...

New page

'''Unreleased structure'''

The entry 5h9v is ON HOLD

Authors: Yokogawa, M., Tsushima, T., Adachi, W., Noda, N.N., Inagaki, F.

Description: Crystal structure of Regnase PIN domain, form I.
[[Category: Unreleased Structures]]
[[Category: Adachi, W]]
[[Category: Inagaki, F]]
[[Category: Yokogawa, M]]
[[Category: Tsushima, T]]
[[Category: Noda, N.N]]