5has - Revision history

OCA at 12:31, 6 March 2024

OCA — Wed, 06 Mar 2024 12:31:29 GMT

←Older revision		Revision as of 12:31, 6 March 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A140UHP8_9PEZI A0A140UHP8_9PEZI]		[https://www.uniprot.org/uniprot/A0A140UHP8_9PEZI A0A140UHP8_9PEZI]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Golgi complex is the central sorting compartment of eukaryotic cells. Arf guanine nucleotide exchange factors (Arf-GEFs) regulate virtually all traffic through the Golgi by activating Arf GTPase trafficking pathways. The Golgi Arf-GEFs contain multiple autoregulatory domains, but the precise mechanisms underlying their function remain largely undefined. We report a crystal structure revealing that the N-terminal DCB and HUS regulatory domains of the Arf-GEF Sec7 form a single structural unit. We demonstrate that the established role of the N-terminal region in dimerization is not conserved; instead, a C-terminal autoinhibitory domain is responsible for dimerization of Sec7. We find that the DCB/HUS domain amplifies the ability of Sec7 to activate Arf1 on the membrane surface by facilitating membrane insertion of the Arf1 amphipathic helix. This enhancing function of the Sec7 N-terminal domains is consistent with the high rate of Arf1-dependent trafficking to the plasma membrane necessary for maximal cell growth.
-
-	The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.,Richardson BC, Halaby SL, Gustafson MA, Fromme JC Elife. 2016 Jan 14;5. pii: e12411. doi: 10.7554/eLife.12411. PMID:26765562<ref>PMID:26765562</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5has" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:29, 9 August 2023

OCA — Wed, 09 Aug 2023 07:29:07 GMT

←Older revision		Revision as of 07:29, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='5has' size='340' side='right'caption='[[5has]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5has' size='340' side='right'caption='[[5has]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5has]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HAS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5has]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothielavioides_terrestris Thermothielavioides terrestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HAS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [~~http~~://pdbe.org/5has PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5has PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5has ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.653Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [https://pdbe.org/5has PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [https://www.ebi.ac.uk/pdbsum/5has PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5has ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A140UHP8_9PEZI A0A140UHP8_9PEZI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Fromme, J C~~]]	+	[[Category: Thermothielavioides terrestris]]
-	[[Category: ~~Richardson, B C~~]]	+	[[Category: Fromme JC]]
-	[[Category: ~~Arf-gef]]~~	+	[[Category: Richardson BC]]
-	~~[[Category: Armadillo]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Tgn]]~~	+
-	~~[[Category: Transport~~]]	+

OCA at 11:39, 25 December 2019

OCA — Wed, 25 Dec 2019 11:39:51 GMT

←Older revision		Revision as of 11:39, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7==		==Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7==
-	<StructureSection load='5has' size='340' side='right' caption='[[5has]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='5has' size='340' side='right'caption='[[5has]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5has]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Acremonium_alabamense Acremonium alabamense]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HAS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5has]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HAS FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Sec7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35720 Acremonium alabamense])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [http://pdbe.org/5has PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [http://www.ebi.ac.uk/pdbsum/5has PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5has ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [http://pdbe.org/5has PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [http://www.ebi.ac.uk/pdbsum/5has PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5has ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acremonium alabamense~~]]	+	[[Category: Large Structures]]
	[[Category: Fromme, J C]]		[[Category: Fromme, J C]]
	[[Category: Richardson, B C]]		[[Category: Richardson, B C]]

OCA at 12:46, 6 November 2017

OCA — Mon, 06 Nov 2017 12:46:09 GMT

←Older revision		Revision as of 12:46, 6 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7==		==Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7==
	<StructureSection load='5has' size='340' side='right' caption='[[5has]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5has' size='340' side='right' caption='[[5has]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5has]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HAS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5has]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Acremonium_alabamense Acremonium alabamense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HAS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [http://pdbe.org/5has PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [http://www.ebi.ac.uk/pdbsum/5has PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Sec7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35720 Acremonium alabamense])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [http://pdbe.org/5has PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [http://www.ebi.ac.uk/pdbsum/5has PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5has ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acremonium alabamense]]
	[[Category: Fromme, J C]]		[[Category: Fromme, J C]]
	[[Category: Richardson, B C]]		[[Category: Richardson, B C]]

OCA at 02:22, 28 January 2016

OCA — Thu, 28 Jan 2016 02:22:16 GMT

←Older revision		Revision as of 02:22, 28 January 2016
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7==
		+	<StructureSection load='5has' size='340' side='right' caption='[[5has]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5has]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HAS FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [http://pdbe.org/5has PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [http://www.ebi.ac.uk/pdbsum/5has PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Golgi complex is the central sorting compartment of eukaryotic cells. Arf guanine nucleotide exchange factors (Arf-GEFs) regulate virtually all traffic through the Golgi by activating Arf GTPase trafficking pathways. The Golgi Arf-GEFs contain multiple autoregulatory domains, but the precise mechanisms underlying their function remain largely undefined. We report a crystal structure revealing that the N-terminal DCB and HUS regulatory domains of the Arf-GEF Sec7 form a single structural unit. We demonstrate that the established role of the N-terminal region in dimerization is not conserved; instead, a C-terminal autoinhibitory domain is responsible for dimerization of Sec7. We find that the DCB/HUS domain amplifies the ability of Sec7 to activate Arf1 on the membrane surface by facilitating membrane insertion of the Arf1 amphipathic helix. This enhancing function of the Sec7 N-terminal domains is consistent with the high rate of Arf1-dependent trafficking to the plasma membrane necessary for maximal cell growth.

-	The ~~entry 5has is ON HOLD until Paper Publication~~	+	The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.,Richardson BC, Halaby SL, Gustafson MA, Fromme JC Elife. 2016 Jan 14;5. pii: e12411. doi: 10.7554/eLife.12411. PMID:26765562<ref>PMID:26765562</ref>

-	~~Authors: Richardson~~, B.C., Fromme, J.C.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	<div class="pdbe-citations 5has" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: ~~Fromme, J.C~~]]	+	<references/>
-	[[Category: ~~Richardson, B.C~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Fromme, J C]]
		+	[[Category: Richardson, B C]]
		+	[[Category: Arf-gef]]
		+	[[Category: Armadillo]]
		+	[[Category: Protein transport]]
		+	[[Category: Tgn]]
		+	[[Category: Transport]]

OCA at 15:11, 20 January 2016

OCA — Wed, 20 Jan 2016 15:11:06 GMT

←Older revision		Revision as of 15:11, 20 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5has is ON HOLD	+	The entry 5has is ON HOLD until Paper Publication

	Authors: Richardson, B.C., Fromme, J.C.		Authors: Richardson, B.C., Fromme, J.C.

OCA: Protected "5has" [edit=sysop:move=sysop]

OCA — Thu, 07 Jan 2016 10:25:21 GMT

Protected "5has" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:25, 7 January 2016

OCA: New page: '''Unreleased structure''' The entry 5has is ON HOLD Authors: Richardson, B.C., Fromme, J.C. Description: Category: Unreleased Structures Category: Fromme, J.C [[Category: Ric...

OCA — Thu, 07 Jan 2016 10:25:20 GMT

New page: '''Unreleased structure''' The entry 5has is ON HOLD Authors: Richardson, B.C., Fromme, J.C. Description: Category: Unreleased Structures Category: Fromme, J.C [[Category: Ric...

New page

'''Unreleased structure'''

The entry 5has is ON HOLD

Authors: Richardson, B.C., Fromme, J.C.

Description:
[[Category: Unreleased Structures]]
[[Category: Fromme, J.C]]
[[Category: Richardson, B.C]]