5hek - Revision history

OCA at 07:33, 9 August 2023

OCA — Wed, 09 Aug 2023 07:33:28 GMT

←Older revision		Revision as of 07:33, 9 August 2023
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	<StructureSection load='5hek' size='340' side='right'caption='[[5hek]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5hek' size='340' side='right'caption='[[5hek]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hek]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Campylobacter_pylori Campylobacter~~ pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HEK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HEK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hek]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HEK FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5hfj\|5hfj]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">HP_0050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=85962 Campylobacter pylori])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hek OCA], [https://pdbe.org/5hek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hek RCSB], [https://www.ebi.ac.uk/pdbsum/5hek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hek ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hek OCA], [~~http~~://pdbe.org/5hek PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hek RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hek PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hek ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O24891_HELPY O24891_HELPY]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Campylobacter~~ pylori]]	+	[[Category: Helicobacter pylori 26695]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Liu, W]]	+	[[Category: Liu W]]
-	[[Category: Ma, B]]	+	[[Category: Ma B]]
-	[[Category: Zhang, H]]	+	[[Category: Zhang H]]
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: M1 hpyavi~~]]	+

OCA at 07:02, 20 May 2020

OCA — Wed, 20 May 2020 07:02:38 GMT

←Older revision		Revision as of 07:02, 20 May 2020
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	==crystal structure of M1.HpyAVI==		==crystal structure of M1.HpyAVI==
-	<StructureSection load='5hek' size='340' side='right' caption='[[5hek]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5hek' size='340' side='right'caption='[[5hek]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hek]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HEK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HEK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hek]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HEK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HEK FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hfj\|5hfj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hfj\|5hfj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hek OCA], [http://pdbe.org/5hek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hek RCSB], [http://www.ebi.ac.uk/pdbsum/5hek PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hek ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HP_0050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hek OCA], [http://pdbe.org/5hek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hek RCSB], [http://www.ebi.ac.uk/pdbsum/5hek PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hek ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5hek" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5hek" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA methyltransferase 3D structures\|DNA methyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter pylori]]
		+	[[Category: Large Structures]]
	[[Category: Liu, W]]		[[Category: Liu, W]]
	[[Category: Ma, B]]		[[Category: Ma, B]]

OCA at 03:25, 10 December 2016

OCA — Sat, 10 Dec 2016 03:25:53 GMT

←Older revision		Revision as of 03:25, 10 December 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5hek is ~~ON HOLD until Paper~~ Publication	+	==crystal structure of M1.HpyAVI==
		+	<StructureSection load='5hek' size='340' side='right' caption='[[5hek]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5hek]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HEK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HEK FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hfj\|5hfj]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hek OCA], [http://pdbe.org/5hek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hek RCSB], [http://www.ebi.ac.uk/pdbsum/5hek PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hek ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DNA N6-methyladenine modification plays an important role in regulating a variety of biological functions in bacteria. However, the mechanism of sequence-specific recognition in N6-methyladenine modification remains elusive. M1.HpyAVI, a DNA N6-adenine methyltransferase from Helicobacter pylori, shows more promiscuous substrate specificity than other enzymes. Here, we present the crystal structures of cofactor-free and AdoMet-bound structures of this enzyme, which were determined at resolutions of 3.0 A and 3.1 A, respectively. The core structure of M1.HpyAVI resembles the canonical AdoMet-dependent MTase fold, while the putative DNA binding regions considerably differ from those of the other MTases, which may account for the substrate promiscuity of this enzyme. Site-directed mutagenesis experiments identified residues D29 and E216 as crucial amino acids for cofactor binding and the methyl transfer activity of the enzyme, while P41, located in a highly flexible loop, playing a determinant role for substrate specificity. Taken together, our data revealed the structural basis underlying DNA N6-adenine methyltransferase substrate promiscuity.

-	~~Authors: Ma~~, B., ~~Zhang~~, H., ~~Liu~~, W.	+	Biochemical and structural characterization of a DNA N6-adenine methyltransferase from Helicobacter pylori.,Ma B, Ma J, Liu D, Guo L, Chen H, Ding J, Liu W, Zhang H Oncotarget. 2016 Jul 5;7(27):40965-40977. doi: 10.18632/oncotarget.9692. PMID:27259995<ref>PMID:27259995</ref>

-	~~Description: crystal structure~~ of M1.~~HpyAVI~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Zhang, H]]~~	+	<div class="pdbe-citations 5hek" style="background-color:#fffaf0;"></div>
-	~~[[Category: Ma, B]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Liu, W]]		[[Category: Liu, W]]
		+	[[Category: Ma, B]]
		+	[[Category: Zhang, H]]
		+	[[Category: Dna binding protein]]
		+	[[Category: M1 hpyavi]]

OCA at 13:41, 3 February 2016

OCA — Wed, 03 Feb 2016 13:41:27 GMT

←Older revision		Revision as of 13:41, 3 February 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hek is ON HOLD	+	The entry 5hek is ON HOLD until Paper Publication

-	Authors: ~~BoMa~~, ~~Hongquan~~ Zhang, ~~Wei~~ Liu	+	Authors: Ma, B., Zhang, H., Liu, W.

	Description: crystal structure of M1.HpyAVI		Description: crystal structure of M1.HpyAVI
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Boma, Hongquan~~ Zhang, ~~Wei~~ Liu]]	+	[[Category: Zhang, H]]
		+	[[Category: Ma, B]]
		+	[[Category: Liu, W]]

OCA: Protected "5hek" [edit=sysop:move=sysop]

OCA — Wed, 13 Jan 2016 18:56:08 GMT

Protected "5hek" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:56, 13 January 2016

OCA: New page: '''Unreleased structure''' The entry 5hek is ON HOLD Authors: BoMa, Hongquan Zhang, Wei Liu Description: crystal structure of M1.HpyAVI Category: Unreleased Structures [[Category: ...

OCA — Wed, 13 Jan 2016 18:56:06 GMT

New page: '''Unreleased structure''' The entry 5hek is ON HOLD Authors: BoMa, Hongquan Zhang, Wei Liu Description: crystal structure of M1.HpyAVI Category: Unreleased Structures [[Category: ...

New page

'''Unreleased structure'''

The entry 5hek is ON HOLD

Authors: BoMa, Hongquan Zhang, Wei Liu

Description: crystal structure of M1.HpyAVI
[[Category: Unreleased Structures]]
[[Category: Boma, Hongquan Zhang, Wei Liu]]