5hh7 - Revision history

OCA at 09:15, 20 March 2024

OCA — Wed, 20 Mar 2024 09:15:42 GMT

←Older revision		Revision as of 09:15, 20 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ORC1B_ARATH ORC1B_ARATH] Essential protein required for ovules fertilization (PubMed:15634699, PubMed:19171893). Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication (By similarity). H3K4me3 effector that regulates positively the transcription of a subset of genes (PubMed:19171893).[UniProtKB:P54784]<ref>PMID:15634699</ref> <ref>PMID:19171893</ref>		[https://www.uniprot.org/uniprot/ORC1B_ARATH ORC1B_ARATH] Essential protein required for ovules fertilization (PubMed:15634699, PubMed:19171893). Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication (By similarity). H3K4me3 effector that regulates positively the transcription of a subset of genes (PubMed:19171893).[UniProtKB:P54784]<ref>PMID:15634699</ref> <ref>PMID:19171893</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	DNA replication initiation relies on the formation of the origin recognition complex (ORC). The plant ORC subunit 1 (ORC1) protein possesses a conserved N-terminal BAH domain with an embedded plant-specific PHD finger, whose function may be potentially regulated by an epigenetic mechanism. Here, we report structural and biochemical studies on the Arabidopsis thaliana ORC1b BAH-PHD cassette which specifically recognizes the unmodified H3 tail. The crystal structure of ORC1b BAH-PHD cassette in complex with an H3(1-15) peptide reveals a strict requirement for the unmodified state of R2, T3, and K4 on the H3 tail and a novel multivalent BAH and PHD readout mode for H3 peptide recognition. Such recognition may contribute to epigenetic regulation of the initiation of DNA replication.
-
-	Structural Basis for the Unique Multivalent Readout of Unmodified H3 Tail by Arabidopsis ORC1b BAH-PHD Cassette.,Li S, Yang Z, Du X, Liu R, Wilkinson AW, Gozani O, Jacobsen SE, Patel DJ, Du J Structure. 2016 Mar 1;24(3):486-94. doi: 10.1016/j.str.2016.01.004. Epub 2016 Feb, 11. PMID:26876097<ref>PMID:26876097</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5hh7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:36, 9 August 2023

OCA — Wed, 09 Aug 2023 07:36:25 GMT

←Older revision		Revision as of 07:36, 9 August 2023
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	<StructureSection load='5hh7' size='340' side='right'caption='[[5hh7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5hh7' size='340' side='right'caption='[[5hh7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hh7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HH7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hh7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HH7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.901Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ORC1B, UNE13, At4g12620, T1P17.210 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh7 OCA], [~~http~~://pdbe.org/5hh7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hh7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hh7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hh7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh7 OCA], [https://pdbe.org/5hh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hh7 RCSB], [https://www.ebi.ac.uk/pdbsum/5hh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hh7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ORC1B_ARATH ORC1B_ARATH]] Essential protein required for ovules fertilization (PubMed:15634699, PubMed:19171893). Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication (By similarity). H3K4me3 effector that regulates positively the transcription of a subset of genes (PubMed:19171893).[UniProtKB:P54784]<ref>PMID:15634699</ref> <ref>PMID:19171893</ref>	+	[https://www.uniprot.org/uniprot/ORC1B_ARATH ORC1B_ARATH] Essential protein required for ovules fertilization (PubMed:15634699, PubMed:19171893). Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication (By similarity). H3K4me3 effector that regulates positively the transcription of a subset of genes (PubMed:19171893).[UniProtKB:P54784]<ref>PMID:15634699</ref> <ref>PMID:19171893</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Du, J]]	+	[[Category: Du J]]
-	[[Category: Li, S]]	+	[[Category: Li S]]
-	~~[[Category: Bah domain]]~~	+
-	~~[[Category: Multivalent readout]]~~	+
-	~~[[Category: Phd finger]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 07:05, 20 May 2020

OCA — Wed, 20 May 2020 07:05:44 GMT

←Older revision		Revision as of 07:05, 20 May 2020
Line 1:		Line 1:

	==crystal structure of Arabidopsis ORC1b BAH-PHD cassette in complex with unmodified H3 peptide==		==crystal structure of Arabidopsis ORC1b BAH-PHD cassette in complex with unmodified H3 peptide==
-	<StructureSection load='5hh7' size='340' side='right' caption='[[5hh7]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5hh7' size='340' side='right'caption='[[5hh7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hh7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HH7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hh7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HH7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh7 OCA], [http://pdbe.org/5hh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/5hh7 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORC1B, UNE13, At4g12620, T1P17.210 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh7 OCA], [http://pdbe.org/5hh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/5hh7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hh7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
		+	[[Category: Large Structures]]
	[[Category: Du, J]]		[[Category: Du, J]]
	[[Category: Li, S]]		[[Category: Li, S]]

OCA at 03:35, 10 March 2016

OCA — Thu, 10 Mar 2016 03:35:15 GMT

←Older revision		Revision as of 03:35, 10 March 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hh7 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==crystal structure of Arabidopsis ORC1b BAH-PHD cassette in complex with unmodified H3 peptide==
		+	<StructureSection load='5hh7' size='340' side='right' caption='[[5hh7]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5hh7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HH7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hh7 OCA], [http://pdbe.org/5hh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/5hh7 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ORC1B_ARATH ORC1B_ARATH]] Essential protein required for ovules fertilization (PubMed:15634699, PubMed:19171893). Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication (By similarity). H3K4me3 effector that regulates positively the transcription of a subset of genes (PubMed:19171893).[UniProtKB:P54784]<ref>PMID:15634699</ref> <ref>PMID:19171893</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DNA replication initiation relies on the formation of the origin recognition complex (ORC). The plant ORC subunit 1 (ORC1) protein possesses a conserved N-terminal BAH domain with an embedded plant-specific PHD finger, whose function may be potentially regulated by an epigenetic mechanism. Here, we report structural and biochemical studies on the Arabidopsis thaliana ORC1b BAH-PHD cassette which specifically recognizes the unmodified H3 tail. The crystal structure of ORC1b BAH-PHD cassette in complex with an H3(1-15) peptide reveals a strict requirement for the unmodified state of R2, T3, and K4 on the H3 tail and a novel multivalent BAH and PHD readout mode for H3 peptide recognition. Such recognition may contribute to epigenetic regulation of the initiation of DNA replication.

-	~~Authors: Li~~, S., Du, J.	+	Structural Basis for the Unique Multivalent Readout of Unmodified H3 Tail by Arabidopsis ORC1b BAH-PHD Cassette.,Li S, Yang Z, Du X, Liu R, Wilkinson AW, Gozani O, Jacobsen SE, Patel DJ, Du J Structure. 2016 Mar 1;24(3):486-94. doi: 10.1016/j.str.2016.01.004. Epub 2016 Feb, 11. PMID:26876097<ref>PMID:26876097</ref>

-	~~Description: crystal structure~~ of ~~Arabidopsis ORC1b BAH-PHD cassette in complex with unmodified H3 peptide~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Li, S]]~~	+	<div class="pdbe-citations 5hh7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Du, J]]		[[Category: Du, J]]
		+	[[Category: Li, S]]
		+	[[Category: Bah domain]]
		+	[[Category: Multivalent readout]]
		+	[[Category: Phd finger]]
		+	[[Category: Transcription]]

OCA at 00:40, 28 January 2016

OCA — Thu, 28 Jan 2016 00:40:50 GMT

←Older revision		Revision as of 00:40, 28 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hh7 is ON HOLD	+	The entry 5hh7 is ON HOLD until Paper Publication

	Authors: Li, S., Du, J.		Authors: Li, S., Du, J.

OCA: Protected "5hh7" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2016 15:25:37 GMT

Protected "5hh7" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:25, 20 January 2016

OCA: New page: '''Unreleased structure''' The entry 5hh7 is ON HOLD Authors: Li, S., Du, J. Description: crystal structure of Arabidopsis ORC1b BAH-PHD cassette in complex with unmodified H3 peptide ...

OCA — Wed, 20 Jan 2016 15:25:36 GMT

New page: '''Unreleased structure''' The entry 5hh7 is ON HOLD Authors: Li, S., Du, J. Description: crystal structure of Arabidopsis ORC1b BAH-PHD cassette in complex with unmodified H3 peptide ...

New page

'''Unreleased structure'''

The entry 5hh7 is ON HOLD

Authors: Li, S., Du, J.

Description: crystal structure of Arabidopsis ORC1b BAH-PHD cassette in complex with unmodified H3 peptide
[[Category: Unreleased Structures]]
[[Category: Li, S]]
[[Category: Du, J]]