5hhz - Revision history

OCA at 07:37, 9 August 2023

OCA — Wed, 09 Aug 2023 07:37:03 GMT

←Older revision		Revision as of 07:37, 9 August 2023
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	<StructureSection load='5hhz' size='340' side='right'caption='[[5hhz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5hhz' size='340' side='right'caption='[[5hhz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hhz]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Maize Maize~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HHZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HHZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hhz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HHZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~, ~~<scene name='pdbligand=ZME:6-(3-METHYL-1H-PYRROL-1-YL)-9H-PURINE'>ZME</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[4o95\|4o95]], [[4oal\|4oal~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=ZME:6-(3-METHYL-1H-PYRROL-1-YL)-9H-PURINE'>ZME</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CKX4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~4577 MAIZE])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hhz OCA], [https://pdbe.org/5hhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hhz RCSB], [https://www.ebi.ac.uk/pdbsum/5hhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hhz ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~1~~.5.99.12 1.5.99.12]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hhz OCA], [~~http~~://pdbe.org/5hhz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hhz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hhz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hhz ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/E3T1W8_MAIZE E3T1W8_MAIZE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cytokinin dehydrogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Maize~~]]	+	[[Category: Zea mays]]
-	[[Category: Briozzo, P]]	+	[[Category: Briozzo P]]
-	[[Category: Kopecny, D]]	+	[[Category: Kopecny D]]
-	~~[[Category: Flavoenzyme]]~~	+
-	~~[[Category: Oxidase/dehydrogenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Plant hormone degradation]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 07:06, 20 May 2020

OCA — Wed, 20 May 2020 07:06:34 GMT

←Older revision		Revision as of 07:06, 20 May 2020
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	==Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with 6-(3-methylpyrrol-1-yl)-9H-purine==		==Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with 6-(3-methylpyrrol-1-yl)-9H-purine==
-	<StructureSection load='5hhz' size='340' side='right' caption='[[5hhz]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5hhz' size='340' side='right'caption='[[5hhz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hhz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HHZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HHZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hhz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HHZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HHZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=ZME:6-(3-METHYL-1H-PYRROL-1-YL)-9H-PURINE'>ZME</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=ZME:6-(3-METHYL-1H-PYRROL-1-YL)-9H-PURINE'>ZME</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o95\|4o95]], [[4oal\|4oal]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o95\|4o95]], [[4oal\|4oal]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hhz OCA], [http://pdbe.org/5hhz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hhz RCSB], [http://www.ebi.ac.uk/pdbsum/5hhz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hhz OCA], [http://pdbe.org/5hhz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hhz RCSB], [http://www.ebi.ac.uk/pdbsum/5hhz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hhz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Cytokinin dehydrogenase]]		[[Category: Cytokinin dehydrogenase]]
		+	[[Category: Large Structures]]
		+	[[Category: Maize]]
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
	[[Category: Kopecny, D]]		[[Category: Kopecny, D]]

OCA at 03:38, 11 May 2016

OCA — Wed, 11 May 2016 03:38:51 GMT

←Older revision		Revision as of 03:38, 11 May 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5hhz is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with 6-(3-methylpyrrol-1-yl)-9H-purine==
		+	<StructureSection load='5hhz' size='340' side='right' caption='[[5hhz]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5hhz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HHZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HHZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=ZME:6-(3-METHYL-1H-PYRROL-1-YL)-9H-PURINE'>ZME</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o95\|4o95]], [[4oal\|4oal]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hhz OCA], [http://pdbe.org/5hhz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hhz RCSB], [http://www.ebi.ac.uk/pdbsum/5hhz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytokinins (CKs) are an important group of phytohormones. Their tightly regulated and balanced levels are essential for proper cell division and plant organ development. Here we report precise quantification of CK metabolites and other phytohormones in maize reproductive organs in the course of pollination and kernel maturation. A novel enzymatic activity dependent on NADP(+) converting trans-zeatin (tZ) to 6-(3-methylpyrrol-1-yl)purine (MPP) was detected. MPP shows weak anticytokinin properties and inhibition of CK dehydrogenases due to their ability to bind to an active site in the opposite orientation than substrates. Although the physiological significance of tZ side-chain cyclization is not anticipated as the MPP occurrence in maize tissue is very low, properties of the novel CK metabolite indicate its potential for utilization in plant in vitro tissue culture. Furthermore, feeding experiments with different isoprenoid CKs revealed distinct preferences in glycosylation of tZ and cis-zeatin (cZ). While tZ is preferentially glucosylated at the N9 position, cZ forms mainly O-glucosides. Since O-glucosides, in contrast to N9-glucosides, are resistant to irreversible cleavage catalyzed by CK dehydrogenases, the observed preference of maize CK glycosyltransferases to O-glycosylate zeatin in the cis-position might be a reason why cZ derivatives are over-accumulated in different maize tissues and organs.

-	~~Authors~~: ~~Kopecny~~, D., Briozzo, P.	+	Cytokinin metabolism in maize: Novel evidence of cytokinin abundance, interconversions and formation of a new trans-zeatin metabolic product with a weak anticytokinin activity.,Hluska T, Dobrev PI, Tarkowska D, Frebortova J, Zalabak D, Kopecny D, Plihal O, Kokas F, Briozzo P, Zatloukal M, Motyka V, Galuszka P Plant Sci. 2016 Jun;247:127-37. doi: 10.1016/j.plantsci.2016.03.014. Epub 2016, Mar 29. PMID:27095406<ref>PMID:27095406</ref>

-	~~Description: Crystal structure~~ of ~~maize cytokinin oxidase~~/~~dehydrogenase 4 (ZmCKO4) in complex with 6~~-(3-~~methylpyrrol-1-yl)-9H-purine~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5hhz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cytokinin dehydrogenase]]
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
	[[Category: Kopecny, D]]		[[Category: Kopecny, D]]
		+	[[Category: Flavoenzyme]]
		+	[[Category: Oxidase/dehydrogenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Plant hormone degradation]]
		+	[[Category: Rossmann fold]]

OCA at 15:57, 26 February 2016

OCA — Fri, 26 Feb 2016 15:57:47 GMT

←Older revision		Revision as of 15:57, 26 February 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hhz is ON HOLD	+	The entry 5hhz is ON HOLD until Paper Publication

	Authors: Kopecny, D., Briozzo, P.		Authors: Kopecny, D., Briozzo, P.

OCA: Protected "5hhz" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2016 15:28:00 GMT

Protected "5hhz" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:28, 20 January 2016

OCA: New page: '''Unreleased structure''' The entry 5hhz is ON HOLD Authors: Kopecny, D., Briozzo, P. Description: Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex wit...

OCA — Wed, 20 Jan 2016 15:27:58 GMT

New page: '''Unreleased structure''' The entry 5hhz is ON HOLD Authors: Kopecny, D., Briozzo, P. Description: Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex wit...

New page

'''Unreleased structure'''

The entry 5hhz is ON HOLD

Authors: Kopecny, D., Briozzo, P.

Description: Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with 6-(3-methylpyrrol-1-yl)-9H-purine
[[Category: Unreleased Structures]]
[[Category: Briozzo, P]]
[[Category: Kopecny, D]]