5hih - Revision history

OCA at 07:37, 9 August 2023

OCA — Wed, 09 Aug 2023 07:37:56 GMT

←Older revision		Revision as of 07:37, 9 August 2023
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	<StructureSection load='5hih' size='340' side='right'caption='[[5hih]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='5hih' size='340' side='right'caption='[[5hih]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hih]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mers Mers~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HIH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hih]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Middle_East_respiratory_syndrome-related_coronavirus Middle East respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HIH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [~~http~~://pdbe.org/5hih PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hih PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hih ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.66Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [https://pdbe.org/5hih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [https://www.ebi.ac.uk/pdbsum/5hih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hih ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/R1A_MERS1 R1A_MERS1] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.[UniProtKB:P0C6X7] Promotes the degradation of host mRNAs by inducing an endonucleolytic RNA cleavage in template mRNAs, and inhibits of host mRNA translation, a function that is separable from its RNA cleavage activity. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.<ref>PMID:26311885</ref> May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates, together with nsp4, in the assembly of virally induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B. signaling.[UniProtKB:P0C6X7]<ref>PMID:25142582</ref> Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7][PROSITE-ProRule:PRU00772] Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[~~SARS Coronavirus Main Proteinase~~\|~~SARS Coronavirus Main Proteinase~~]]	+	*[[Virus protease 3D structures\|Virus protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mers~~]]	+	[[Category: Middle East respiratory syndrome-related coronavirus]]
-	[[Category: Hilgenfeld, R]]	+	[[Category: Hilgenfeld R]]
-	[[Category: Lei, J]]	+	[[Category: Lei J]]
-	~~[[Category: Macrodomain]]~~	+
-	~~[[Category: Non-structural protein]]~~	+
-	~~[[Category: Sandwich fold]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 08:16, 27 May 2020

OCA — Wed, 27 May 2020 08:16:32 GMT

←Older revision		Revision as of 08:16, 27 May 2020
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	==Crystal structure of the macro domain in Middle-East Respiratory Syndrome Coronavirus==		==Crystal structure of the macro domain in Middle-East Respiratory Syndrome Coronavirus==
-	<StructureSection load='5hih' size='340' side='right' caption='[[5hih]], [[Resolution\|resolution]] 1.66Å' scene=''>	+	<StructureSection load='5hih' size='340' side='right'caption='[[5hih]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hih]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIH OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HIH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hih]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mers Mers]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HIH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [http://pdbe.org/5hih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [http://www.ebi.ac.uk/pdbsum/5hih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hih ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [http://pdbe.org/5hih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [http://www.ebi.ac.uk/pdbsum/5hih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hih ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5hih" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5hih" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[SARS Coronavirus Main Proteinase\|SARS Coronavirus Main Proteinase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mers]]
	[[Category: Hilgenfeld, R]]		[[Category: Hilgenfeld, R]]
	[[Category: Lei, J]]		[[Category: Lei, J]]

OCA at 05:08, 9 September 2016

OCA — Fri, 09 Sep 2016 05:08:45 GMT

←Older revision		Revision as of 05:08, 9 September 2016
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5hih]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HIH FirstGlance]. <br>		<table><tr><td colspan='2'>[[5hih]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HIH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [http://pdbe.org/5hih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [http://www.ebi.ac.uk/pdbsum/5hih PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [http://pdbe.org/5hih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [http://www.ebi.ac.uk/pdbsum/5hih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hih ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:30, 20 June 2016

OCA — Mon, 20 Jun 2016 07:30:03 GMT

←Older revision		Revision as of 07:30, 20 June 2016
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		+
	==Crystal structure of the macro domain in Middle-East Respiratory Syndrome Coronavirus==		==Crystal structure of the macro domain in Middle-East Respiratory Syndrome Coronavirus==
	<StructureSection load='5hih' size='340' side='right' caption='[[5hih]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='5hih' size='340' side='right' caption='[[5hih]], [[Resolution\|resolution]] 1.66Å' scene=''>
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [http://pdbe.org/5hih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [http://www.ebi.ac.uk/pdbsum/5hih PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [http://pdbe.org/5hih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [http://www.ebi.ac.uk/pdbsum/5hih PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The papain-like protease (PLpro) of Middle-East respiratory syndrome coronavirus (MERS-CoV) has proteolytic, deubiquitinating, and deISGylating activities. The latter two are involved in the suppression of the antiviral innate immune response of the host cell. To contribute to an understanding of this process, we present here the X-ray crystal structure of a complex between MERS-CoV PLpro and human ubiquitin (Ub) that is devoid of any covalent linkage between the two proteins. Five regions of the PLpro bind to two areas of the Ub. The C-terminal five residues of Ub, RLRGG, are similar to the P5-P1 residues of the polyprotein substrates of the PLpro and are responsible for the major part of the interaction between the two macromolecules. Through sitedirected mutagenesis, we demonstrate that conserved Asp165 and non-conserved Asp164 are important for the catalytic activities of MERS-CoV PLpro. The enzyme appears not to be optimized for catalytic efficiency; thus, replacement of Phe269 by Tyr leads to increased peptidolytic and deubiquitinating activities. Ubiquitin binding by MERS-CoV PLpro involves remarkable differences compared to the corresponding complex with SARS-CoV PLpro. The structure and the mutational study help understand common and unique features of the deubiquitinating activity of MERS-CoV PLpro.
		+
		+	Structural and mutational analysis of the interaction between the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease and human ubiquitin.,Lei J, Hilgenfeld R Virol Sin. 2016 May 30. PMID:27245450<ref>PMID:27245450</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hih" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA: Protected "5hih" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2016 17:29:07 GMT

Protected "5hih" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:29, 20 January 2016

OCA: New page: ==Crystal structure of the macro domain in Middle-East Respiratory Syndrome Coronavirus== == Structural highlights ==
<table><tr><td colspan='2'>[[5hih]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HIH FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hih OCA], [http://pdbe.org/5hih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hih RCSB], [http://www.ebi.ac.uk/pdbsum/5hih PDBsum]</span></td></tr>
</table>
TOC
</StructureSection>
[[Category: Hilgenfeld, R]]
[[Category: Lei, J]]
[[Category: Macrodomain]]
[[Category: Non-structural protein]]
[[Category: Sandwich fold]]
[[Category: Viral protein]]