5hl3 - Revision history

OCA at 10:45, 16 August 2023

OCA — Wed, 16 Aug 2023 10:45:31 GMT

←Older revision		Revision as of 10:45, 16 August 2023
Line 3:		Line 3:
	<StructureSection load='5hl3' size='340' side='right'caption='[[5hl3]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='5hl3' size='340' side='right'caption='[[5hl3]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hl3]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lismo Lismo~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HL3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HL3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hl3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HL3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HL3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">lmo2470 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169963 LISMO])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hl3 OCA], [https://pdbe.org/5hl3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hl3 RCSB], [https://www.ebi.ac.uk/pdbsum/5hl3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hl3 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hl3 OCA], [~~http~~://pdbe.org/5hl3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hl3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hl3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hl3 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8Y4H2_LISMO Q8Y4H2_LISMO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During pregnancy, the placenta protects the fetus against the maternal immune response, as well as bacterial and viral pathogens. Bacterial pathogens that have evolved specific mechanisms of breaching this barrier, such as Listeria monocytogenes, present a unique opportunity for learning how the placenta carries out its protective function. We previously identified the L. monocytogenes protein Internalin P (InlP) as a secreted virulence factor critical for placental infection. Here, we show that InlP, but not the highly similar L. monocytogenes internalin Lmo2027, binds to human afadin (encoded by AF-6), a protein associated with cell-cell junctions. A crystal structure of InlP reveals several unique features, including an extended leucine-rich repeat (LRR) domain with a distinctive Ca2+-binding site. Despite afadin's involvement in the formation of cell-cell junctions, MDCK epithelial cells expressing InlP displayed a decrease in the magnitude of the traction stresses they could exert on deformable substrates, similar to the decrease in traction exhibited by AF-6 knock-out MDCK cells. L. monocytogenes DeltainlP mutants were deficient in their ability to form actin-rich protrusions from the basal face of polarized epithelial monolayers, a necessary step in the crossing of such monolayers (transcytosis). A similar phenotype was observed for bacteria expressing an internal in-frame deletion in inlP (inlP DeltaLRR5) that specifically disrupts its interaction with afadin. However, afadin deletion in the host cells did not rescue the transcytosis defect. We conclude that secreted InlP targets cytosolic afadin to specifically promote L. monocytogenes transcytosis across the basal face of epithelial monolayers, which may contribute to the crossing of the basement membrane during placental infection.
		+
		+	Listeria monocytogenes InlP interacts with afadin and facilitates basement membrane crossing.,Faralla C, Bastounis EE, Ortega FE, Light SH, Rizzuto G, Gao L, Marciano DK, Nocadello S, Anderson WF, Robbins JR, Theriot JA, Bakardjiev AI PLoS Pathog. 2018 May 30;14(5):e1007094. doi: 10.1371/journal.ppat.1007094. , eCollection 2018 May. PMID:29847585<ref>PMID:29847585</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hl3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lismo~~]]	+	[[Category: Listeria monocytogenes EGD-e]]
-	[[Category: Anderson~~, W F~~]]	+	[[Category: Anderson WF]]
-	[[Category: Bakardjiev, A]]	+	[[Category: Bakardjiev A]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Faralla C]]
-	[[Category: Faralla, C]]	+	[[Category: Kiryukhina O]]
-	[[Category: Kiryukhina, O]]	+	[[Category: Kwon K]]
-	[[Category: Kwon, K]]	+	[[Category: Light SH]]
-	[[Category: Light~~, S H~~]]	+	[[Category: Minasov G]]
-	[[Category: Minasov, G]]	+	[[Category: Nocadello S]]
-	[[Category: Nocadello, S]]	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Internalin]]~~	+
-	~~[[Category: Lrr]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 08:20, 27 May 2020

OCA — Wed, 27 May 2020 08:20:37 GMT

←Older revision		Revision as of 08:20, 27 May 2020
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	==Crystal structure of Listeria monocytogenes InlP==		==Crystal structure of Listeria monocytogenes InlP==
-	<StructureSection load='5hl3' size='340' side='right' caption='[[5hl3]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='5hl3' size='340' side='right'caption='[[5hl3]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hl3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HL3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HL3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hl3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lismo Lismo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HL3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HL3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hl3 OCA], [http://pdbe.org/5hl3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hl3 RCSB], [http://www.ebi.ac.uk/pdbsum/5hl3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hl3 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lmo2470 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169963 LISMO])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hl3 OCA], [http://pdbe.org/5hl3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hl3 RCSB], [http://www.ebi.ac.uk/pdbsum/5hl3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hl3 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lismo]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Bakardjiev, A]]		[[Category: Bakardjiev, A]]

OCA at 00:42, 19 January 2017

OCA — Thu, 19 Jan 2017 00:42:15 GMT

←Older revision		Revision as of 00:42, 19 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hl3 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Listeria monocytogenes InlP==
-		+	<StructureSection load='5hl3' size='340' side='right' caption='[[5hl3]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5hl3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HL3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HL3 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hl3 OCA], [http://pdbe.org/5hl3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hl3 RCSB], [http://www.ebi.ac.uk/pdbsum/5hl3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hl3 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Anderson, W F]]
		+	[[Category: Bakardjiev, A]]
		+	[[Category: Structural genomic]]
		+	[[Category: Faralla, C]]
		+	[[Category: Kiryukhina, O]]
		+	[[Category: Kwon, K]]
		+	[[Category: Light, S H]]
		+	[[Category: Minasov, G]]
		+	[[Category: Nocadello, S]]
		+	[[Category: Csgid]]
		+	[[Category: Internalin]]
		+	[[Category: Lrr]]
		+	[[Category: Unknown function]]

OCA at 13:41, 15 May 2016

OCA — Sun, 15 May 2016 13:41:49 GMT

←Older revision		Revision as of 13:41, 15 May 2016
Line 3:		Line 3:
	The entry 5hl3 is ON HOLD until Paper Publication		The entry 5hl3 is ON HOLD until Paper Publication

-	Authors: ~~Nocadello, S., Light, S.H., Minasov, G., Kiryukhina, O., Kwon, K., Faralla, C., Bakardjiev, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Light, S.H]]
-	[[Category: Bakardjiev, A]]
-	[[Category: Minasov, G]]
-	[[Category: Faralla, C]]
-	[[Category: Kiryukhina, O]]
-	[[Category: Anderson, W.F]]
-	[[Category: Nocadello, S]]
-	[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
-	[[Category: Kwon, K]]

OCA at 15:25, 10 February 2016

OCA — Wed, 10 Feb 2016 15:25:02 GMT

←Older revision		Revision as of 15:25, 10 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hl3 is ON HOLD	+	The entry 5hl3 is ON HOLD until Paper Publication

	Authors: Nocadello, S., Light, S.H., Minasov, G., Kiryukhina, O., Kwon, K., Faralla, C., Bakardjiev, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)		Authors: Nocadello, S., Light, S.H., Minasov, G., Kiryukhina, O., Kwon, K., Faralla, C., Bakardjiev, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

OCA at 00:49, 28 January 2016

OCA — Thu, 28 Jan 2016 00:49:18 GMT

←Older revision		Revision as of 00:49, 28 January 2016
Line 3:		Line 3:
	The entry 5hl3 is ON HOLD		The entry 5hl3 is ON HOLD

-	Authors: Nocadello, S. , Light, S.H., Minasov, G., Kiryukhina, O., Kwon, K., Faralla, C, Bakardjiev. A, Anderson, W.F.	+	Authors: Nocadello, S., Light, S.H., Minasov, G., Kiryukhina, O., Kwon, K., Faralla, C., Bakardjiev, A., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Nocadello, S. ,~~ Light, S.H]]	+	[[Category: Light, S.H]]
-	[[Category: ~~Faralla~~, ~~C, Bakardjiev.~~ A~~, Anderson, W.F~~]]	+	[[Category: Bakardjiev, A]]
	[[Category: Minasov, G]]		[[Category: Minasov, G]]
		+	[[Category: Faralla, C]]
	[[Category: Kiryukhina, O]]		[[Category: Kiryukhina, O]]
		+	[[Category: Anderson, W.F]]
		+	[[Category: Nocadello, S]]
		+	[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
	[[Category: Kwon, K]]		[[Category: Kwon, K]]

OCA: Protected "5hl3" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2016 15:41:29 GMT

Protected "5hl3" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:41, 20 January 2016

OCA: New page: '''Unreleased structure''' The entry 5hl3 is ON HOLD Authors: Nocadello, S. , Light, S.H., Minasov, G., Kiryukhina, O., Kwon, K., Faralla, C, Bakardjiev. A, Anderson, W.F. Description:...

OCA — Wed, 20 Jan 2016 15:41:28 GMT

New page: '''Unreleased structure''' The entry 5hl3 is ON HOLD Authors: Nocadello, S. , Light, S.H., Minasov, G., Kiryukhina, O., Kwon, K., Faralla, C, Bakardjiev. A, Anderson, W.F. Description:...

New page

'''Unreleased structure'''

The entry 5hl3 is ON HOLD

Authors: Nocadello, S. , Light, S.H., Minasov, G., Kiryukhina, O., Kwon, K., Faralla, C, Bakardjiev. A, Anderson, W.F.

Description:
[[Category: Unreleased Structures]]
[[Category: Nocadello, S. , Light, S.H]]
[[Category: Faralla, C, Bakardjiev. A, Anderson, W.F]]
[[Category: Minasov, G]]
[[Category: Kiryukhina, O]]
[[Category: Kwon, K]]