5hok - Revision history

OCA at 10:49, 16 August 2023

OCA — Wed, 16 Aug 2023 10:49:04 GMT

←Older revision		Revision as of 10:49, 16 August 2023
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	==MamB-CTD mutant - D247A==		==MamB-CTD mutant - D247A==
-	<StructureSection load='5hok' size='340' side='right' caption='[[5hok]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5hok' size='340' side='right'caption='[[5hok]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hok]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Magnetospira_sp.~~_qh~~-2 Magnetospira sp. qh-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HOK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hok]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Magnetospira_sp._QH-2 Magnetospira sp. QH-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HOK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~mamB, MGMAQ_1061 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288970 Magnetospira sp. QH-2])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hok OCA], [~~http~~://pdbe.org/5hok PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hok RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hok PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hok ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hok OCA], [https://pdbe.org/5hok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hok RCSB], [https://www.ebi.ac.uk/pdbsum/5hok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hok ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MAMB_MAGSQ MAMB_MAGSQ] Plays a dual, essential role in magnetosome formation; required for magnetosome vesicle formation as well as biomineralization (By similarity). Probably binds and transports iron (Probable). Requires heterodimerization with MamM for stability (By similarity).[UniProtKB:V6F510]<ref>PMID:29243866</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Magnetospira sp. qh-2]]	+	[[Category: Large Structures]]
-	[[Category: Keren, N]]	+	[[Category: Magnetospira sp. QH-2]]
-	[[Category: Zarivach, R]]	+	[[Category: Keren N]]
-	[[Category: Zeytuni, N]]	+	[[Category: Zarivach R]]
-	~~[[Category: C-terminal domain]]~~	+	[[Category: Zeytuni N]]
-	~~[[Category: Cation diffusion facilitator]]~~	+
-	~~[[Category: Magnetotactic bacteria]]~~	+
-	~~[[Category: Metal transport]]~~	+
-	~~[[Category: Metal-binding site]]~~	+
-	~~[[Category: Metallochaperone like domain~~]]	+

OCA at 06:54, 15 February 2018

OCA — Thu, 15 Feb 2018 06:54:54 GMT

←Older revision		Revision as of 06:54, 15 February 2018
Line 3:		Line 3:
	<StructureSection load='5hok' size='340' side='right' caption='[[5hok]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5hok' size='340' side='right' caption='[[5hok]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hok]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HOK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hok]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Magnetospira_sp._qh-2 Magnetospira sp. qh-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HOK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mamB, MGMAQ_1061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288970 Magnetospira sp. QH-2])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hok OCA], [http://pdbe.org/5hok PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hok RCSB], [http://www.ebi.ac.uk/pdbsum/5hok PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hok ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hok OCA], [http://pdbe.org/5hok PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hok RCSB], [http://www.ebi.ac.uk/pdbsum/5hok PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hok ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Magnetospirillum gryphiswaldense MSR-1 synthesizes membrane-enclosed magnetite (Fe3 O4 ) nanoparticles, magnetosomes, for magnetotaxis. Formation of these organelles involves a complex process comprising key steps which are governed by specific magnetosome-associated proteins. MamB, a cation diffusion facilitator (CDF) family member has been implicated in magnetosome-directed iron transport. However, deletion mutagenesis studies revealed that MamB is essential for the formation of magnetosome membrane vesicles, but its precise role remains elusive. In this study, we employed a multi-disciplinary approach to define the role of MamB during magnetosome formation. Using site-directed mutagenesis complemented by structural analyses, fluorescence microscopy and cryo-electron tomography, we show that MamB is most likely an active magnetosome-directed transporter serving two distinct, yet essential functions. First, MamB initiates magnetosome vesicle formation in a transport-independent process, probably by serving as a landmark protein. Second, MamB transport activity is required for magnetite nucleation. Furthermore, by determining the crystal structure of the MamB cytosolic C-terminal domain, we also provide mechanistic insight into transport regulation. Additionally, we present evidence that magnetosome vesicle growth and chain formation are independent of magnetite nucleation and magnetic interactions respectively. Together, our data provide novel insight into the role of the key bifunctional magnetosome protein MamB, and the early steps of magnetosome formation.
		+
		+	The dual role of MamB in magnetosome membrane assembly and magnetite biomineralization.,Uebe R, Keren-Khadmy N, Zeytuni N, Katzmann E, Navon Y, Davidov G, Bitton R, Plitzko JM, Schuler D, Zarivach R Mol Microbiol. 2018 Feb;107(4):542-557. doi: 10.1111/mmi.13899. Epub 2018 Jan 9. PMID:29243866<ref>PMID:29243866</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hok" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Magnetospira sp. qh-2]]
	[[Category: Keren, N]]		[[Category: Keren, N]]
	[[Category: Zarivach, R]]		[[Category: Zarivach, R]]

OCA at 18:04, 1 February 2017

OCA — Wed, 01 Feb 2017 18:04:07 GMT

←Older revision		Revision as of 18:04, 1 February 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5hok is ~~ON HOLD until Jan 19 2018~~	+	==MamB-CTD mutant - D247A==
-		+	<StructureSection load='5hok' size='340' side='right' caption='[[5hok]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~Authors~~: ~~Keren~~, N., ~~Zeytuni~~, N., ~~Zarivach~~, R.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5hok]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HOK FirstGlance]. <br>
-	~~Description: MamB-CTD mutant -D247A~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hok OCA], [http://pdbe.org/5hok PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hok RCSB], [http://www.ebi.ac.uk/pdbsum/5hok PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hok ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Keren, N]]
	[[Category: Zarivach, R]]		[[Category: Zarivach, R]]
	[[Category: Zeytuni, N]]		[[Category: Zeytuni, N]]
-	[[Category: ~~Keren, N~~]]	+	[[Category: C-terminal domain]]
		+	[[Category: Cation diffusion facilitator]]
		+	[[Category: Magnetotactic bacteria]]
		+	[[Category: Metal transport]]
		+	[[Category: Metal-binding site]]
		+	[[Category: Metallochaperone like domain]]

OCA at 14:05, 3 February 2016

OCA — Wed, 03 Feb 2016 14:05:22 GMT

←Older revision		Revision as of 14:05, 3 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hok is ON HOLD	+	The entry 5hok is ON HOLD until Jan 19 2018

-	Authors: Keren, N., Zeytuni, N.	+	Authors: Keren, N., Zeytuni, N., Zarivach, R.

	Description: MamB-CTD mutant -D247A		Description: MamB-CTD mutant -D247A
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Zarivach, R]]
	[[Category: Zeytuni, N]]		[[Category: Zeytuni, N]]
	[[Category: Keren, N]]		[[Category: Keren, N]]

OCA: Protected "5hok" [edit=sysop:move=sysop]

OCA — Thu, 28 Jan 2016 00:59:10 GMT

Protected "5hok" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:59, 28 January 2016

OCA: New page: '''Unreleased structure''' The entry 5hok is ON HOLD Authors: Keren, N., Zeytuni, N. Description: MamB-CTD mutant -D247A Category: Unreleased Structures Category: Zeytuni, N [[...

OCA — Thu, 28 Jan 2016 00:59:09 GMT

New page: '''Unreleased structure''' The entry 5hok is ON HOLD Authors: Keren, N., Zeytuni, N. Description: MamB-CTD mutant -D247A Category: Unreleased Structures Category: Zeytuni, N [[...

New page

'''Unreleased structure'''

The entry 5hok is ON HOLD

Authors: Keren, N., Zeytuni, N.

Description: MamB-CTD mutant -D247A
[[Category: Unreleased Structures]]
[[Category: Zeytuni, N]]
[[Category: Keren, N]]