5hs0 - Revision history

OCA at 10:52, 16 August 2023

OCA — Wed, 16 Aug 2023 10:52:23 GMT

←Older revision		Revision as of 10:52, 16 August 2023
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	<StructureSection load='5hs0' size='340' side='right'caption='[[5hs0]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5hs0' size='340' side='right'caption='[[5hs0]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hs0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HS0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HS0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hs0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HS0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hs0 OCA], [~~http~~://pdbe.org/5hs0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hs0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hs0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hs0 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hs0 OCA], [https://pdbe.org/5hs0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hs0 RCSB], [https://www.ebi.ac.uk/pdbsum/5hs0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hs0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Baker, D]]	+	[[Category: Baker D]]
-	[[Category: Cascio, D]]	+	[[Category: Cascio D]]
-	[[Category: Fallas~~, J A~~]]	+	[[Category: Fallas JA]]
-	[[Category: McNamara~~, D E~~]]	+	[[Category: McNamara DE]]
-	[[Category: Yeates~~, T O]]~~	+	[[Category: Yeates TO]]
-	~~[[Category: De novo protein]]~~	+
-	~~[[Category: Designed oligomeric interface]]~~	+
-	~~[[Category: Protein design~~]]	+

OCA at 07:36, 27 November 2019

OCA — Wed, 27 Nov 2019 07:36:06 GMT

←Older revision		Revision as of 07:36, 27 November 2019
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	==Computationally Designed Cyclic Tetramer ank1C4_7==		==Computationally Designed Cyclic Tetramer ank1C4_7==
-	<StructureSection load='5hs0' size='340' side='right' caption='[[5hs0]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5hs0' size='340' side='right'caption='[[5hs0]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hs0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HS0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hs0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HS0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hs0 OCA], [http://pdbe.org/5hs0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hs0 RCSB], [http://www.ebi.ac.uk/pdbsum/5hs0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hs0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hs0 OCA], [http://pdbe.org/5hs0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hs0 RCSB], [http://www.ebi.ac.uk/pdbsum/5hs0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hs0 ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Baker, D]]		[[Category: Baker, D]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]

OCA at 11:37, 12 April 2017

OCA — Wed, 12 Apr 2017 11:37:25 GMT

←Older revision		Revision as of 11:37, 12 April 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5hs0 is ~~ON HOLD until Paper~~ Publication	+	==Computationally Designed Cyclic Tetramer ank1C4_7==
		+	<StructureSection load='5hs0' size='340' side='right' caption='[[5hs0]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5hs0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HS0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hs0 OCA], [http://pdbe.org/5hs0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hs0 RCSB], [http://www.ebi.ac.uk/pdbsum/5hs0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hs0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Self-assembling cyclic protein homo-oligomers play important roles in biology, and the ability to generate custom homo-oligomeric structures could enable new approaches to probe biological function. Here we report a general approach to design cyclic homo-oligomers that employs a new residue-pair-transform method to assess the designability of a protein-protein interface. This method is sufficiently rapid to enable the systematic enumeration of cyclically docked arrangements of a monomer followed by sequence design of the newly formed interfaces. We use this method to design interfaces onto idealized repeat proteins that direct their assembly into complexes that possess cyclic symmetry. Of 96 designs that were characterized experimentally, 21 were found to form stable monodisperse homo-oligomers in solution, and 15 (four homodimers, six homotrimers, six homotetramers and one homopentamer) had solution small-angle X-ray scattering data consistent with the design models. X-ray crystal structures were obtained for five of the designs and each is very close to their corresponding computational model.

-	~~Authors: McNamara, D.E~~., ~~Cascio~~, D., ~~Fallas~~, ~~J.A.~~, ~~Baker~~, D., Yeates, T.O.	+	Computational design of self-assembling cyclic protein homo-oligomers.,Fallas JA, Ueda G, Sheffler W, Nguyen V, McNamara DE, Sankaran B, Pereira JH, Parmeggiani F, Brunette TJ, Cascio D, Yeates TR, Zwart P, Baker D Nat Chem. 2017 Apr;9(4):353-360. doi: 10.1038/nchem.2673. Epub 2016 Dec 5. PMID:28338692<ref>PMID:28338692</ref>

-	~~Description: Computationally Designed Cyclic Tetramer ank1C4_7~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5hs0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Baker, D]]		[[Category: Baker, D]]
-	[[Category: Mcnamara, D.E]]
-	[[Category: Yeates, T.O]]
-	[[Category: Fallas, J.A]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]
		+	[[Category: Fallas, J A]]
		+	[[Category: McNamara, D E]]
		+	[[Category: Yeates, T O]]
		+	[[Category: De novo protein]]
		+	[[Category: Designed oligomeric interface]]
		+	[[Category: Protein design]]

OCA at 09:30, 9 March 2017

OCA — Thu, 09 Mar 2017 09:30:16 GMT

←Older revision		Revision as of 09:30, 9 March 2017
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	Description: Computationally Designed Cyclic Tetramer ank1C4_7		Description: Computationally Designed Cyclic Tetramer ank1C4_7
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Baker, D]]
	[[Category: Mcnamara, D.E]]		[[Category: Mcnamara, D.E]]
	[[Category: Yeates, T.O]]		[[Category: Yeates, T.O]]
	[[Category: Fallas, J.A]]		[[Category: Fallas, J.A]]
-	[[Category: Baker, D]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]

OCA at 01:06, 21 February 2016

OCA — Sun, 21 Feb 2016 01:06:28 GMT

←Older revision		Revision as of 01:06, 21 February 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hs0 is ON HOLD	+	The entry 5hs0 is ON HOLD until Paper Publication

	Authors: McNamara, D.E., Cascio, D., Fallas, J.A., Baker, D., Yeates, T.O.		Authors: McNamara, D.E., Cascio, D., Fallas, J.A., Baker, D., Yeates, T.O.

OCA: Protected "5hs0" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2016 14:14:44 GMT

Protected "5hs0" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:14, 3 February 2016

OCA: New page: '''Unreleased structure''' The entry 5hs0 is ON HOLD Authors: McNamara, D.E., Cascio, D., Fallas, J.A., Baker, D., Yeates, T.O. Description: Computationally Designed Cyclic Tetramer an...

OCA — Wed, 03 Feb 2016 14:14:42 GMT

New page: '''Unreleased structure''' The entry 5hs0 is ON HOLD Authors: McNamara, D.E., Cascio, D., Fallas, J.A., Baker, D., Yeates, T.O. Description: Computationally Designed Cyclic Tetramer an...

New page

'''Unreleased structure'''

The entry 5hs0 is ON HOLD

Authors: McNamara, D.E., Cascio, D., Fallas, J.A., Baker, D., Yeates, T.O.

Description: Computationally Designed Cyclic Tetramer ank1C4_7
[[Category: Unreleased Structures]]
[[Category: Mcnamara, D.E]]
[[Category: Yeates, T.O]]
[[Category: Fallas, J.A]]
[[Category: Baker, D]]
[[Category: Cascio, D]]