5hsz - Revision history

OCA at 12:33, 6 March 2024

OCA — Wed, 06 Mar 2024 12:33:05 GMT

←Older revision		Revision as of 12:33, 6 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SLMA_KLEP7 SLMA_KLEP7] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.		[https://www.uniprot.org/uniprot/SLMA_KLEP7 SLMA_KLEP7] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Cell division in most prokaryotes is mediated by FtsZ, which polymerizes to create the cytokinetic Z ring. Multiple FtsZ-binding proteins regulate FtsZ polymerization to ensure the proper spatiotemporal formation of the Z ring at the division site. The DNA-binding protein SlmA binds to FtsZ and prevents Z-ring formation through the nucleoid in a process called "nucleoid occlusion" (NO). As do most FtsZ-accessory proteins, SlmA interacts with the conserved C-terminal domain (CTD) that is connected to the FtsZ core by a long, flexible linker. However, SlmA is distinct from other regulatory factors in that it must be DNA-bound to interact with the FtsZ CTD. Few structures of FtsZ regulator-CTD complexes are available, but all reveal the CTD bound as a helix. To deduce the molecular basis for the unique SlmA-DNA-FtsZ CTD regulatory interaction and provide insight into FtsZ-regulator protein complex formation, we determined structures of Escherichia coli, Vibrio cholera, and Klebsiella pneumonia SlmA-DNA-FtsZ CTD ternary complexes. Strikingly, the FtsZ CTD does not interact with SlmA as a helix but binds as an extended conformation in a narrow, surface-exposed pocket formed only in the DNA-bound state of SlmA and located at the junction between the DNA-binding and C-terminal dimer domains. Binding studies are consistent with the structure and underscore key interactions in complex formation. Combined, these data reveal the molecular basis for the SlmA-DNA-FtsZ interaction with implications for SlmA's NO function and underscore the ability of the FtsZ CTD to adopt a wide range of conformations, explaining its ability to bind diverse regulatory proteins.
-
-	Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ.,Schumacher MA, Zeng W Proc Natl Acad Sci U S A. 2016 May 3;113(18):4988-93. doi:, 10.1073/pnas.1602327113. Epub 2016 Apr 18. PMID:27091999<ref>PMID:27091999</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5hsz" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:53, 16 August 2023

OCA — Wed, 16 Aug 2023 10:53:10 GMT

←Older revision		Revision as of 10:53, 16 August 2023
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	<StructureSection load='5hsz' size='340' side='right'caption='[[5hsz]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5hsz' size='340' side='right'caption='[[5hsz]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hsz]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Klep7 Klep7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HSZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HSZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hsz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_MGH_78578 Klebsiella pneumoniae subsp. pneumoniae MGH 78578] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HSZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~slmA, KPN78578_39420, KPN_03981 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=272620 KLEP7])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hsz OCA], [~~http~~://pdbe.org/5hsz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hsz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hsz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hsz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hsz OCA], [https://pdbe.org/5hsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hsz RCSB], [https://www.ebi.ac.uk/pdbsum/5hsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hsz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SLMA_KLEP7 SLMA_KLEP7]] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.	+	[https://www.uniprot.org/uniprot/SLMA_KLEP7 SLMA_KLEP7] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Klep7~~]]	+	[[Category: Klebsiella pneumoniae subsp. pneumoniae MGH 78578]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Schumacher, M A~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Zeng, W~~]]	+	[[Category: Schumacher MA]]
-	[[Category: ~~Cell cycle]]~~	+	[[Category: Zeng W]]
-	~~[[Category: Cell division]]~~	+
-	~~[[Category: Cytokinesis]]~~	+
-	~~[[Category: Ftsz]]~~	+
-	~~[[Category: Nucleoid occlusion]]~~	+
-	~~[[Category: Slma]]~~	+
-	~~[[Category: Z-ring~~]]	+

OCA at 08:31, 27 May 2020

OCA — Wed, 27 May 2020 08:31:05 GMT

←Older revision		Revision as of 08:31, 27 May 2020
Line 1:		Line 1:

	==Structure of the K. pneumonia SlmA protein bound to the C-terminal tail of the cytoskeletal cell division protein FtsZ==		==Structure of the K. pneumonia SlmA protein bound to the C-terminal tail of the cytoskeletal cell division protein FtsZ==
-	<StructureSection load='5hsz' size='340' side='right' caption='[[5hsz]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5hsz' size='340' side='right'caption='[[5hsz]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hsz]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HSZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HSZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hsz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HSZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HSZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hsz OCA], [http://pdbe.org/5hsz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hsz RCSB], [http://www.ebi.ac.uk/pdbsum/5hsz PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">slmA, KPN78578_39420, KPN_03981 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hsz OCA], [http://pdbe.org/5hsz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hsz RCSB], [http://www.ebi.ac.uk/pdbsum/5hsz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hsz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klep7]]
		+	[[Category: Large Structures]]
	[[Category: Schumacher, M A]]		[[Category: Schumacher, M A]]
	[[Category: Zeng, W]]		[[Category: Zeng, W]]

OCA at 08:09, 1 June 2016

OCA — Wed, 01 Jun 2016 08:09:48 GMT

←Older revision		Revision as of 08:09, 1 June 2016
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SLMA_KLEP7 SLMA_KLEP7]] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.		[[http://www.uniprot.org/uniprot/SLMA_KLEP7 SLMA_KLEP7]] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cell division in most prokaryotes is mediated by FtsZ, which polymerizes to create the cytokinetic Z ring. Multiple FtsZ-binding proteins regulate FtsZ polymerization to ensure the proper spatiotemporal formation of the Z ring at the division site. The DNA-binding protein SlmA binds to FtsZ and prevents Z-ring formation through the nucleoid in a process called "nucleoid occlusion" (NO). As do most FtsZ-accessory proteins, SlmA interacts with the conserved C-terminal domain (CTD) that is connected to the FtsZ core by a long, flexible linker. However, SlmA is distinct from other regulatory factors in that it must be DNA-bound to interact with the FtsZ CTD. Few structures of FtsZ regulator-CTD complexes are available, but all reveal the CTD bound as a helix. To deduce the molecular basis for the unique SlmA-DNA-FtsZ CTD regulatory interaction and provide insight into FtsZ-regulator protein complex formation, we determined structures of Escherichia coli, Vibrio cholera, and Klebsiella pneumonia SlmA-DNA-FtsZ CTD ternary complexes. Strikingly, the FtsZ CTD does not interact with SlmA as a helix but binds as an extended conformation in a narrow, surface-exposed pocket formed only in the DNA-bound state of SlmA and located at the junction between the DNA-binding and C-terminal dimer domains. Binding studies are consistent with the structure and underscore key interactions in complex formation. Combined, these data reveal the molecular basis for the SlmA-DNA-FtsZ interaction with implications for SlmA's NO function and underscore the ability of the FtsZ CTD to adopt a wide range of conformations, explaining its ability to bind diverse regulatory proteins.
		+
		+	Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ.,Schumacher MA, Zeng W Proc Natl Acad Sci U S A. 2016 May 3;113(18):4988-93. doi:, 10.1073/pnas.1602327113. Epub 2016 Apr 18. PMID:27091999<ref>PMID:27091999</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hsz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:29, 12 May 2016

OCA — Thu, 12 May 2016 11:29:37 GMT

←Older revision		Revision as of 11:29, 12 May 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hsz is ~~ON HOLD until Paper Publication~~	+	==Structure of the K. pneumonia SlmA protein bound to the C-terminal tail of the cytoskeletal cell division protein FtsZ==
-		+	<StructureSection load='5hsz' size='340' side='right' caption='[[5hsz]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~Authors~~: ~~Zeng~~, W., ~~Schumacher~~, M.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5hsz]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HSZ FirstGlance]. <br>
-	~~Description:~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hsz OCA], [http://pdbe.org/5hsz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hsz RCSB], [http://www.ebi.ac.uk/pdbsum/5hsz PDBsum]</span></td></tr>
-	[[Category: Schumacher, M.A]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SLMA_KLEP7 SLMA_KLEP7]] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Schumacher, M A]]
	[[Category: Zeng, W]]		[[Category: Zeng, W]]
		+	[[Category: Cell cycle]]
		+	[[Category: Cell division]]
		+	[[Category: Cytokinesis]]
		+	[[Category: Ftsz]]
		+	[[Category: Nucleoid occlusion]]
		+	[[Category: Slma]]
		+	[[Category: Z-ring]]

OCA at 01:08, 21 February 2016

OCA — Sun, 21 Feb 2016 01:08:23 GMT

←Older revision		Revision as of 01:08, 21 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hsz is ON HOLD	+	The entry 5hsz is ON HOLD until Paper Publication

-	Authors: Schumacher, M.A~~., Zeng, W~~.	+	Authors: Zeng, W., Schumacher, M.A.

	Description:		Description:

OCA: Protected "5hsz" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2016 14:17:49 GMT

Protected "5hsz" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:17, 3 February 2016

OCA: New page: '''Unreleased structure''' The entry 5hsz is ON HOLD Authors: Schumacher, M.A., Zeng, W. Description: Category: Unreleased Structures Category: Schumacher, M.A [[Category: Zen...

OCA — Wed, 03 Feb 2016 14:17:48 GMT

New page: '''Unreleased structure''' The entry 5hsz is ON HOLD Authors: Schumacher, M.A., Zeng, W. Description: Category: Unreleased Structures Category: Schumacher, M.A [[Category: Zen...

New page

'''Unreleased structure'''

The entry 5hsz is ON HOLD

Authors: Schumacher, M.A., Zeng, W.

Description:
[[Category: Unreleased Structures]]
[[Category: Schumacher, M.A]]
[[Category: Zeng, W]]