5hub - Revision history

OCA at 10:54, 16 August 2023

OCA — Wed, 16 Aug 2023 10:54:08 GMT

←Older revision		Revision as of 10:54, 16 August 2023
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	==High-resolution structure of chorismate mutase from Corynebacterium glutamicum==		==High-resolution structure of chorismate mutase from Corynebacterium glutamicum==
-	<StructureSection load='5hub' size='340' side='right' caption='[[5hub]], [[Resolution\|resolution]] 1.06Å' scene=''>	+	<StructureSection load='5hub' size='340' side='right'caption='[[5hub]], [[Resolution\|resolution]] 1.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hub]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HUB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HUB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=FMT:FORMIC+ACID'>FMT</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.06Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Cgl0853 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1718~~ "~~Micrococcus glutamicus~~" ~~Kinoshita et al. 1958])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hub OCA], [https://pdbe.org/5hub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hub RCSB], [https://www.ebi.ac.uk/pdbsum/5hub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hub ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hub OCA], [~~http~~://pdbe.org/5hub PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hub RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hub PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hub ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8NS29_CORGL Q8NS29_CORGL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5hub" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5hub" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D structures of chorismate mutase\|3D structures of chorismate mutase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Micrococcus glutamicus kinoshita et al. 1958~~]]	+	[[Category: Corynebacterium glutamicum]]
-	[[Category: ~~Chorismate mutase~~]]	+	[[Category: Large Structures]]
-	[[Category: Burschowsky, D]]	+	[[Category: Burschowsky D]]
-	[[Category: Heim~~, J B~~]]	+	[[Category: Heim JB]]
-	[[Category: Krengel, U]]	+	[[Category: Krengel U]]
-	[[Category: Thorbjoernsrud~~, H V]]~~	+	[[Category: Thorbjoernsrud HV]]
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Pericyclic reaction]]~~	+
-	~~[[Category: Shikimate pathway~~]]	+

OCA at 06:35, 20 December 2017

OCA — Wed, 20 Dec 2017 06:35:52 GMT

←Older revision		Revision as of 06:35, 20 December 2017
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hub OCA], [http://pdbe.org/5hub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hub RCSB], [http://www.ebi.ac.uk/pdbsum/5hub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hub ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hub OCA], [http://pdbe.org/5hub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hub RCSB], [http://www.ebi.ac.uk/pdbsum/5hub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hub ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Corynebacterium glutamicum is widely used for the industrial production of amino acids, nucleotides, and vitamins. The shikimate pathway enzymes DAHP synthase (CgDS; Cg2391) and chorismate mutase (CgCM; Cgl0853) play a key role for the biosynthesis of aromatic compounds. Here we show that CgCM requires the formation of a complex with CgDS to achieve full activity, and that both CgCM and CgDS are feedback regulated by aromatic amino acids binding to CgDS. Kinetic analysis showed that Phe and Tyr inhibit CgCM activity by inter-enzyme allostery, whereas Trp binding to CgDS strongly activates CgCM. Mechanistic insights were gained from crystal structures of the CgCM homodimer, tetrameric CgDS, and the heterooctameric CgCM-CgDS complex, refined to 1.1, 2.5, and 2.2 A resolution, respectively. Structural details from the allosteric binding sites reveal that DAHP synthase is recruited as the dominant regulatory platform to control the shikimate pathway, similar to the corresponding enzyme complex from Mycobacterium tuberculosis.
		+
		+	Inter-enzyme allosteric regulation of chorismate mutase in Corynebacterium glutamicum: Structural basis of feedback activation by Trp.,Burschowsky D, Thorbjornsrud HV, Heim JB, Fahrig-Kamarauskaite J, Wurth-Roderer K, Kast P, Krengel U Biochemistry. 2017 Nov 27. doi: 10.1021/acs.biochem.7b01018. PMID:29178787<ref>PMID:29178787</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hub" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:54, 13 November 2017

OCA — Mon, 13 Nov 2017 07:54:15 GMT

←Older revision		Revision as of 07:54, 13 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hub is ~~ON HOLD until Paper Publication~~	+	==High-resolution structure of chorismate mutase from Corynebacterium glutamicum==
-		+	<StructureSection load='5hub' size='340' side='right' caption='[[5hub]], [[Resolution\|resolution]] 1.06Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5hub]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_glutamicus"_kinoshita_et_al._1958 "micrococcus glutamicus" kinoshita et al. 1958]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUB FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cgl0853 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1718 "Micrococcus glutamicus" Kinoshita et al. 1958])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hub OCA], [http://pdbe.org/5hub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hub RCSB], [http://www.ebi.ac.uk/pdbsum/5hub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hub ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Micrococcus glutamicus kinoshita et al. 1958]]
		+	[[Category: Chorismate mutase]]
		+	[[Category: Burschowsky, D]]
		+	[[Category: Heim, J B]]
		+	[[Category: Krengel, U]]
		+	[[Category: Thorbjoernsrud, H V]]
		+	[[Category: Isomerase]]
		+	[[Category: Pericyclic reaction]]
		+	[[Category: Shikimate pathway]]

OCA at 13:55, 15 May 2016

OCA — Sun, 15 May 2016 13:55:18 GMT

←Older revision		Revision as of 13:55, 15 May 2016
Line 3:		Line 3:
	The entry 5hub is ON HOLD until Paper Publication		The entry 5hub is ON HOLD until Paper Publication

-	Authors: ~~Burschowsky, D., Heim, J.B., Thorbjoernsrud, H.V., Krengel, U.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Thorbjoernsrud, H.V]]
-	[[Category: Heim, J.B]]
-	[[Category: Krengel, U]]
-	[[Category: Burschowsky, D]]

OCA: Protected "5hub" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2016 14:21:46 GMT

Protected "5hub" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:21, 3 February 2016

OCA: New page: '''Unreleased structure''' The entry 5hub is ON HOLD until Paper Publication Authors: Burschowsky, D., Heim, J.B., Thorbjoernsrud, H.V., Krengel, U. Description: [[Category: Unrelease...

OCA — Wed, 03 Feb 2016 14:21:45 GMT

New page: '''Unreleased structure''' The entry 5hub is ON HOLD until Paper Publication Authors: Burschowsky, D., Heim, J.B., Thorbjoernsrud, H.V., Krengel, U. Description: [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 5hub is ON HOLD until Paper Publication

Authors: Burschowsky, D., Heim, J.B., Thorbjoernsrud, H.V., Krengel, U.

Description:
[[Category: Unreleased Structures]]
[[Category: Thorbjoernsrud, H.V]]
[[Category: Heim, J.B]]
[[Category: Krengel, U]]
[[Category: Burschowsky, D]]