5huw - Revision history

OCA at 10:54, 16 August 2023

OCA — Wed, 16 Aug 2023 10:54:40 GMT

←Older revision		Revision as of 10:54, 16 August 2023
Line 3:		Line 3:
	<StructureSection load='5huw' size='340' side='right'caption='[[5huw]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='5huw' size='340' side='right'caption='[[5huw]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5huw]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hhv-1 Hhv-~~1] and [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HUW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5huw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1 Human alphaherpesvirus 1] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HUW FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[3q5u\|3q5u]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">Kpna2, Rch1 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5huw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huw OCA], [https://pdbe.org/5huw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5huw RCSB], [https://www.ebi.ac.uk/pdbsum/5huw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5huw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5huw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huw OCA], [~~http~~://pdbe.org/5huw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5huw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5huw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5huw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.	+	[https://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hhv-~~1]]	+	[[Category: Human alphaherpesvirus 1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Cingolani, G]]	+	[[Category: Cingolani G]]
-	[[Category: Lokareddy~~, R K~~]]	+	[[Category: Lokareddy RK]]
-	[[Category: Sankhala~~, R S]]~~	+	[[Category: Sankhala RS]]
-	~~[[Category: Herpes virus]]~~	+
-	~~[[Category: Importin alpha-1]]~~	+
-	~~[[Category: Nl]]~~	+
-	~~[[Category: Nuclear import]]~~	+
-	~~[[Category: Tranport protein]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 11:43, 25 December 2019

OCA — Wed, 25 Dec 2019 11:43:50 GMT

←Older revision		Revision as of 11:43, 25 December 2019
Line 1:		Line 1:

	==Structure of HSV-1 Large Terminase NLS bound to importin alpha==		==Structure of HSV-1 Large Terminase NLS bound to importin alpha==
-	<StructureSection load='5huw' size='340' side='right' caption='[[5huw]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='5huw' size='340' side='right'caption='[[5huw]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5huw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5huw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hhv-1 Hhv-1] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q5u\|3q5u]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q5u\|3q5u]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kpna2, Rch1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huw OCA], [http://pdbe.org/5huw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huw RCSB], [http://www.ebi.ac.uk/pdbsum/5huw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5huw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huw OCA], [http://pdbe.org/5huw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huw RCSB], [http://www.ebi.ac.uk/pdbsum/5huw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5huw ProSAT]</span></td></tr>
	</table>		</table>
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5huw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5huw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Importin 3D structures\|Importin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hhv-1]]
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Cingolani, G]]		[[Category: Cingolani, G]]
	[[Category: Lokareddy, R K]]		[[Category: Lokareddy, R K]]

OCA at 17:29, 15 November 2017

OCA — Wed, 15 Nov 2017 17:29:27 GMT

←Older revision		Revision as of 17:29, 15 November 2017
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5huw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5huw" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Importin\|Importin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:37, 21 September 2017

OCA — Thu, 21 Sep 2017 04:37:59 GMT

←Older revision		Revision as of 04:37, 21 September 2017
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[5huw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUW FirstGlance]. <br>		<table><tr><td colspan='2'>[[5huw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q5u\|3q5u]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q5u\|3q5u]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huw OCA], [http://pdbe.org/5huw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huw RCSB], [http://www.ebi.ac.uk/pdbsum/5huw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huw OCA], [http://pdbe.org/5huw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huw RCSB], [http://www.ebi.ac.uk/pdbsum/5huw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5huw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5huw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5huw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Importin\|Importin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:05, 10 May 2016

OCA — Tue, 10 May 2016 19:05:52 GMT

←Older revision		Revision as of 19:05, 10 May 2016
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.		[[http://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The tripartite terminase complex of herperviruses assembles in the cytoplasm of infected cells and exploits the host nuclear import machinery to gain access to the nucleus, where capsid assembly and genome-packaging occur. Here, we analyzed the structure and conservation of Nuclear Localization Signal (NLS) sequences previously identified in Herpes Simplex Virus 1 (HSV-1) large terminase and Human Cytomegalovirus (HCMV) small terminase. We found a monopartite NLS at the N-terminus of large terminase, flanking the ATPase domain, that is conserved only in alpha-herpesviruses. In contrast, small terminase exposes a classical NLS at the far C-terminus of its helical structure, which is conserved only in two genera of the beta-subfamily and absent in alpha- and gamma-herpesviruses. In addition, we predicted a classical NLS in the third terminase subunit that is partially conserved among herpesviruses. Bioinformatic analysis revealed that both location and potency of NLSs in terminase subunits evolved more rapidly than the rest of the aminoacid sequence, despite the selective pressure to keep terminase gene products active and localized in the nucleus. We propose that swapping NLSs among terminase subunits is a regulatory mechanism that allows different herpesviruses to regulate the kinetics of terminase nuclear import, reflecting a mechanism of virus:host adaptation.
		+
		+	Divergent Evolution of Nuclear Localization Signal Sequences in Herpesvirus Terminase Subunits.,Sankhala RS, Lokareddy RK, Cingolani G J Biol Chem. 2016 Mar 31. pii: jbc.M116.724393. PMID:27033706<ref>PMID:27033706</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5huw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 03:59, 10 March 2016

OCA — Thu, 10 Mar 2016 03:59:45 GMT

←Older revision		Revision as of 03:59, 10 March 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5huw is ON HOLD~~	+	==Structure of HSV-1 Large Terminase NLS bound to importin alpha==
-		+	<StructureSection load='5huw' size='340' side='right' caption='[[5huw]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~Authors: Sankhala, R.S., Lokareddy, R.K., Cingolani, G.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5huw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HUW FirstGlance]. <br>
-	~~Description:~~ Structure of HSV-1 Large Terminase NLS bound to importin alpha	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q5u\|3q5u]]</td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5huw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5huw OCA], [http://pdbe.org/5huw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5huw RCSB], [http://www.ebi.ac.uk/pdbsum/5huw PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Lokareddy~~, R.K]]	+	</table>
-	[[~~Category~~: ~~Sankhala, R~~.S]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
		+	__TOC__
		+	</StructureSection>
	[[Category: Cingolani, G]]		[[Category: Cingolani, G]]
		+	[[Category: Lokareddy, R K]]
		+	[[Category: Sankhala, R S]]
		+	[[Category: Herpes virus]]
		+	[[Category: Importin alpha-1]]
		+	[[Category: Nl]]
		+	[[Category: Nuclear import]]
		+	[[Category: Tranport protein]]
		+	[[Category: Viral protein]]

OCA at 16:24, 26 February 2016

OCA — Fri, 26 Feb 2016 16:24:48 GMT

←Older revision		Revision as of 16:24, 26 February 2016
Line 3:		Line 3:
	The entry 5huw is ON HOLD		The entry 5huw is ON HOLD

-	Authors: Sankhala, R.S~~., Cingolani, G~~., Lokareddy, R.K.	+	Authors: Sankhala, R.S., Lokareddy, R.K., Cingolani, G.

	Description: Structure of HSV-1 Large Terminase NLS bound to importin alpha		Description: Structure of HSV-1 Large Terminase NLS bound to importin alpha

OCA: Protected "5huw" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2016 14:23:20 GMT

Protected "5huw" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:23, 3 February 2016

OCA: New page: '''Unreleased structure''' The entry 5huw is ON HOLD Authors: Sankhala, R.S., Cingolani, G., Lokareddy, R.K. Description: Structure of HSV-1 Large Terminase NLS bound to importin alpha...

OCA — Wed, 03 Feb 2016 14:23:19 GMT

New page: '''Unreleased structure''' The entry 5huw is ON HOLD Authors: Sankhala, R.S., Cingolani, G., Lokareddy, R.K. Description: Structure of HSV-1 Large Terminase NLS bound to importin alpha...

New page

'''Unreleased structure'''

The entry 5huw is ON HOLD

Authors: Sankhala, R.S., Cingolani, G., Lokareddy, R.K.

Description: Structure of HSV-1 Large Terminase NLS bound to importin alpha
[[Category: Unreleased Structures]]
[[Category: Lokareddy, R.K]]
[[Category: Sankhala, R.S]]
[[Category: Cingolani, G]]