5hvi - Revision history

OCA at 10:55, 16 August 2023

OCA — Wed, 16 Aug 2023 10:55:28 GMT

←Older revision		Revision as of 10:55, 16 August 2023
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	<StructureSection load='5hvi' size='340' side='right'caption='[[5hvi]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='5hvi' size='340' side='right'caption='[[5hvi]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hvi]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HVI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HVI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hvi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HVI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HVI FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5hw5\|5hw5]], [[5hw1\|5hw1]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.64Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">bla, blaT-3, blaT-4, blaT-5, blaT-6 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hvi OCA], [https://pdbe.org/5hvi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hvi RCSB], [https://www.ebi.ac.uk/pdbsum/5hvi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hvi ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hvi OCA], [~~http~~://pdbe.org/5hvi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hvi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hvi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hvi ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX]] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.	+	[https://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dmochowski~~, I J~~]]	+	[[Category: Dmochowski IJ]]
-	[[Category: Roose~~, B W]]~~	+	[[Category: Roose BW]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 11:44, 25 December 2019

OCA — Wed, 25 Dec 2019 11:44:02 GMT

←Older revision		Revision as of 11:44, 25 December 2019
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	==Crystal structure of TEM1 beta-lactamase==		==Crystal structure of TEM1 beta-lactamase==
-	<StructureSection load='5hvi' size='340' side='right' caption='[[5hvi]], [[Resolution\|resolution]] 1.64Å' scene=''>	+	<StructureSection load='5hvi' size='340' side='right'caption='[[5hvi]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5hvi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HVI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HVI FirstGlance]. <br>		<table><tr><td colspan='2'>[[5hvi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HVI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HVI FirstGlance]. <br>
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]	+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Dmochowski, I J]]		[[Category: Dmochowski, I J]]
	[[Category: Roose, B W]]		[[Category: Roose, B W]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 12:20, 16 January 2019

OCA — Wed, 16 Jan 2019 12:20:45 GMT

←Older revision		Revision as of 12:20, 16 January 2019
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX]] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.		[[http://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX]] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Genetically encoded (GE) contrast agents detectable by magnetic resonance imaging (MRI) enable non-invasive visualization of gene expression and cell proliferation at virtually unlimited penetration depths. Using hyperpolarized (129) Xe in combination with chemical exchange saturation transfer, an MR contrast approach known as hyper-CEST, enables ultrasensitive protein detection and biomolecular imaging. GE MRI contrast agents developed to date include nanoscale proteinaceous gas vesicles as well as the monomeric bacterial proteins TEM-1 beta-lactamase (bla) and maltose binding protein (MBP). To improve understanding of hyper-CEST NMR with proteins, structural and computational studies were performed to further characterize the Xe-bla interaction. X-ray crystallography validated the location of a high-occupancy Xe binding site predicted by MD simulations, and mutagenesis experiments confirmed this Xe site as the origin of the observed CEST contrast. Structural studies and MD simulations with representative bla mutants offered additional insight regarding the relationship between local protein structure and CEST contrast.
		+
		+	A Structural Basis for (129) Xe Hyper-CEST Signal in TEM-1 beta-Lactamase.,Roose BW, Zemerov SD, Wang Y, Kasimova MA, Carnevale V, Dmochowski IJ Chemphyschem. 2018 Aug 27. doi: 10.1002/cphc.201800624. PMID:30151973<ref>PMID:30151973</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5hvi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:49, 6 November 2017

OCA — Mon, 06 Nov 2017 10:49:12 GMT

←Older revision		Revision as of 10:49, 6 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hvi is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of TEM1 beta-lactamase==
-		+	<StructureSection load='5hvi' size='340' side='right' caption='[[5hvi]], [[Resolution\|resolution]] 1.64Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5hvi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HVI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HVI FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hw5\|5hw5]], [[5hw1\|5hw1]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, blaT-3, blaT-4, blaT-5, blaT-6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hvi OCA], [http://pdbe.org/5hvi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hvi RCSB], [http://www.ebi.ac.uk/pdbsum/5hvi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hvi ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX]] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Beta-lactamase]]
		+	[[Category: Dmochowski, I J]]
		+	[[Category: Roose, B W]]
		+	[[Category: Hydrolase]]

OCA at 13:56, 15 May 2016

OCA — Sun, 15 May 2016 13:56:39 GMT

←Older revision		Revision as of 13:56, 15 May 2016
Line 3:		Line 3:
	The entry 5hvi is ON HOLD until Paper Publication		The entry 5hvi is ON HOLD until Paper Publication

-	Authors: ~~Roose, B.W., Dmochowski, I.J.~~	+	Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Dmochowski, I.J]]
-	[[Category: Roose, B.W]]

OCA at 01:13, 21 February 2016

OCA — Sun, 21 Feb 2016 01:13:32 GMT

←Older revision		Revision as of 01:13, 21 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hvi is ON HOLD	+	The entry 5hvi is ON HOLD until Paper Publication

	Authors: Roose, B.W., Dmochowski, I.J.		Authors: Roose, B.W., Dmochowski, I.J.

OCA: Protected "5hvi" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2016 14:25:28 GMT

Protected "5hvi" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:25, 3 February 2016

OCA: New page: '''Unreleased structure''' The entry 5hvi is ON HOLD Authors: Roose, B.W., Dmochowski, I.J. Description: Category: Unreleased Structures Category: Dmochowski, I.J [[Category: ...

OCA — Wed, 03 Feb 2016 14:25:26 GMT

New page: '''Unreleased structure''' The entry 5hvi is ON HOLD Authors: Roose, B.W., Dmochowski, I.J. Description: Category: Unreleased Structures Category: Dmochowski, I.J [[Category: ...

New page

'''Unreleased structure'''

The entry 5hvi is ON HOLD

Authors: Roose, B.W., Dmochowski, I.J.

Description:
[[Category: Unreleased Structures]]
[[Category: Dmochowski, I.J]]
[[Category: Roose, B.W]]