5hwk - Revision history

OCA at 10:57, 16 August 2023

OCA — Wed, 16 Aug 2023 10:57:13 GMT

←Older revision		Revision as of 10:57, 16 August 2023
Line 3:		Line 3:
	<StructureSection load='5hwk' size='340' side='right'caption='[[5hwk]], [[Resolution\|resolution]] 1.34Å' scene=''>		<StructureSection load='5hwk' size='340' side='right'caption='[[5hwk]], [[Resolution\|resolution]] 1.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hwk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HWK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5HWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hwk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HWK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.344Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5hwi\|5hwi]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">GCG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hwk OCA], [https://pdbe.org/5hwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hwk RCSB], [https://www.ebi.ac.uk/pdbsum/5hwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hwk ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5hwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hwk OCA], [~~http~~://pdbe.org/5hwk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hwk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hwk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hwk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CHAC_YEAST CHAC_YEAST]] Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Allows utilization of gluthathione through subsequent cleavage of the Cys-Gly dipeptide by Cys-Gly metallodipeptidase DUG1.<ref>PMID:23070364</ref>	+	[https://www.uniprot.org/uniprot/CHAC_YEAST CHAC_YEAST] Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Allows utilization of gluthathione through subsequent cleavage of the Cys-Gly dipeptide by Cys-Gly metallodipeptidase DUG1.<ref>PMID:23070364</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bachhawat~~, A K~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Chandel, A]]	+	[[Category: Bachhawat AK]]
-	[[Category: Gautam, R]]	+	[[Category: Chandel A]]
-	[[Category: Karthikeyan, S]]	+	[[Category: Gautam R]]
-	[[Category: Kaur, A]]	+	[[Category: Karthikeyan S]]
-	[[Category: Kumar, A]]	+	[[Category: Kaur A]]
-	[[Category: Srivastava, R]]	+	[[Category: Kumar A]]
-	~~[[Category: Chac]]~~	+	[[Category: Srivastava R]]
-	~~[[Category: Gcg1]]~~	+
-	~~[[Category: Glutathione]]~~	+
-	~~[[Category: Oxo-proline]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Yer163c~~]]	+

OCA at 08:34, 27 May 2020

OCA — Wed, 27 May 2020 08:34:47 GMT

←Older revision		Revision as of 08:34, 27 May 2020
Line 1:		Line 1:

	==Crystal structure of gama glutamyl cyclotransferease specific to glutathione from yeast==		==Crystal structure of gama glutamyl cyclotransferease specific to glutathione from yeast==
-	<StructureSection load='5hwk' size='340' side='right' caption='[[5hwk]], [[Resolution\|resolution]] 1.34Å' scene=''>	+	<StructureSection load='5hwk' size='340' side='right'caption='[[5hwk]], [[Resolution\|resolution]] 1.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hwk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HWK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HWK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hwk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HWK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HWK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hwi\|5hwi]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hwi\|5hwi]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hwk OCA], [http://pdbe.org/5hwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hwk RCSB], [http://www.ebi.ac.uk/pdbsum/5hwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hwk ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GCG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hwk OCA], [http://pdbe.org/5hwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hwk RCSB], [http://www.ebi.ac.uk/pdbsum/5hwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hwk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Bachhawat, A K]]		[[Category: Bachhawat, A K]]
	[[Category: Chandel, A]]		[[Category: Chandel, A]]

OCA at 19:06, 15 December 2016

OCA — Thu, 15 Dec 2016 19:06:10 GMT

←Older revision		Revision as of 19:06, 15 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hwk is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of gama glutamyl cyclotransferease specific to glutathione from yeast==
		+	<StructureSection load='5hwk' size='340' side='right' caption='[[5hwk]], [[Resolution\|resolution]] 1.34Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5hwk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HWK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HWK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5hwi\|5hwi]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hwk OCA], [http://pdbe.org/5hwk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hwk RCSB], [http://www.ebi.ac.uk/pdbsum/5hwk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hwk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHAC_YEAST CHAC_YEAST]] Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Allows utilization of gluthathione through subsequent cleavage of the Cys-Gly dipeptide by Cys-Gly metallodipeptidase DUG1.<ref>PMID:23070364</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutathione degradation plays an important role in glutathione and redox homeostasis and thus it is imperative to understand the enzymes and the mechanisms involved in glutathione degradation in detail. We describe here ChaC2, a member of the ChaC family of gamma-glutamylcyclotransferases, as an enzyme that degrades glutathione in the cytosol of mammalian cells. ChaC2 is distinct from the previously described ChaC1, to which ChaC2 shows about 50% sequence identity. Human and mouse ChaC2 proteins, purified in vitro, show 10-20 fold lower catalytic efficiency than ChaC1, although they showed comparable Km values (Km of 3.7+/-0.4 mM and kcat of 15.9+/-1.0 min-1 towards glutathione for human ChaC2; Km of 2.2+/-0.4 mM and kcat of 225.2+/-15 min-1 towards glutathione for human ChaC1). The ChaC1 and ChaC2 proteins also shared the same specificity for reduced glutathione, with no activity against either gamma-glutamyl amino acids or oxidized glutathione. The ChaC2 proteins were found to be expressed constitutively in cells, unlike the tightly regulated ChaC1. Moreover, lower eukaryotes have a single member of the ChaC family that appears to be orthologous to ChaC2. In addition, we determined the crystal structure of yeast ChaC2 homologue, GCG1 at 1.34 A resolution which represents the first structure of the ChaC family of proteins. The catalytic site is defined by a fortuitous benzoic acid molecule bound to the crystal structure. The mechanism for binding and catalytic activity of this new enzyme of glutathione degradation, which is involved in continuous, but basal turnover of cytosolic glutathione, is proposed.

-	~~Authors~~: ~~Kaur~~, A., Gautam, R., Srivastava, R., Chandel, A., Kumar, A., Karthikeyan, S., Bachhawat~~, A~~.K.	+	ChaC2: An Enzyme for Slow Turnover of Cytosolic Glutathione.,Kaur A, Gautam R, Srivastava R, Chandel A, Kumar A, Karthikeyan S, Bachhawat AK J Biol Chem. 2016 Dec 2. pii: jbc.M116.727479. PMID:27913623<ref>PMID:27913623</ref>

-	~~Description: Crystal structure~~ of ~~gama glutamyl cyclotransferease specific to glutathione from yeast~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5hwk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bachhawat, A K]]
		+	[[Category: Chandel, A]]
	[[Category: Gautam, R]]		[[Category: Gautam, R]]
	[[Category: Karthikeyan, S]]		[[Category: Karthikeyan, S]]
-	[[Category: Kumar, A]]
-	[[Category: Chandel, A]]
	[[Category: Kaur, A]]		[[Category: Kaur, A]]
-	[[Category: ~~Bachhawat~~, A.K]]	+	[[Category: Kumar, A]]
	[[Category: Srivastava, R]]		[[Category: Srivastava, R]]
		+	[[Category: Chac]]
		+	[[Category: Gcg1]]
		+	[[Category: Glutathione]]
		+	[[Category: Oxo-proline]]
		+	[[Category: Transferase]]
		+	[[Category: Yer163c]]

OCA at 16:28, 26 February 2016

OCA — Fri, 26 Feb 2016 16:28:13 GMT

←Older revision		Revision as of 16:28, 26 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5hwk is ON HOLD	+	The entry 5hwk is ON HOLD until Paper Publication

	Authors: Kaur, A., Gautam, R., Srivastava, R., Chandel, A., Kumar, A., Karthikeyan, S., Bachhawat, A.K.		Authors: Kaur, A., Gautam, R., Srivastava, R., Chandel, A., Kumar, A., Karthikeyan, S., Bachhawat, A.K.

OCA at 15:48, 10 February 2016

OCA — Wed, 10 Feb 2016 15:48:34 GMT

←Older revision		Revision as of 15:48, 10 February 2016
Line 3:		Line 3:
	The entry 5hwk is ON HOLD		The entry 5hwk is ON HOLD

-	Authors: ~~Amandeep~~ Kaur, ~~Ruchi~~ Gautam, ~~Ritika~~ Srivastava, ~~Avinash~~ Chandel, ~~Akhilesh~~ Kumar, ~~Subramanian~~ Karthikeyan, ~~Anand K~~.Bachhawat	+	Authors: Kaur, A., Gautam, R., Srivastava, R., Chandel, A., Kumar, A., Karthikeyan, S., Bachhawat, A.K.

	Description: Crystal structure of gama glutamyl cyclotransferease specific to glutathione from yeast		Description: Crystal structure of gama glutamyl cyclotransferease specific to glutathione from yeast
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Amandeep Kaur, Ruchi~~ Gautam, ~~Ritika Srivastava~~, ~~Avinash~~ Chandel, ~~Akhilesh Kumar~~, ~~Subramanian Karthikeyan~~, ~~Anand K~~.~~Bachhawat~~]]	+	[[Category: Gautam, R]]
		+	[[Category: Karthikeyan, S]]
		+	[[Category: Kumar, A]]
		+	[[Category: Chandel, A]]
		+	[[Category: Kaur, A]]
		+	[[Category: Bachhawat, A.K]]
		+	[[Category: Srivastava, R]]

OCA: Protected "5hwk" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2016 14:28:56 GMT

Protected "5hwk" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:28, 3 February 2016

OCA: New page: '''Unreleased structure''' The entry 5hwk is ON HOLD Authors: Amandeep Kaur, Ruchi Gautam, Ritika Srivastava, Avinash Chandel, Akhilesh Kumar, Subramanian Karthikeyan, Anand K.Bachhawat...

OCA — Wed, 03 Feb 2016 14:28:55 GMT

New page: '''Unreleased structure''' The entry 5hwk is ON HOLD Authors: Amandeep Kaur, Ruchi Gautam, Ritika Srivastava, Avinash Chandel, Akhilesh Kumar, Subramanian Karthikeyan, Anand K.Bachhawat...

New page

'''Unreleased structure'''

The entry 5hwk is ON HOLD

Authors: Amandeep Kaur, Ruchi Gautam, Ritika Srivastava, Avinash Chandel, Akhilesh Kumar, Subramanian Karthikeyan, Anand K.Bachhawat

Description: Crystal structure of gama glutamyl cyclotransferease specific to glutathione from yeast
[[Category: Unreleased Structures]]
[[Category: Amandeep Kaur, Ruchi Gautam, Ritika Srivastava, Avinash Chandel, Akhilesh Kumar, Subramanian Karthikeyan, Anand K.Bachhawat]]