5hy5 - Revision history

OCA at 07:23, 1 May 2024

OCA — Wed, 01 May 2024 07:23:40 GMT

←Older revision		Revision as of 07:23, 1 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/E9P162_9ACTN E9P162_9ACTN]		[https://www.uniprot.org/uniprot/E9P162_9ACTN E9P162_9ACTN]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Flavin-dependent halogenase enzymes are potentially useful biocatalysts for the regioselectively halogenation of aromatic compounds. Haloaromatic compounds can be utilised in the synthesis and biosynthesis of pharmaceuticals and other valuable products. Here we report the first X-ray crystal structure of a tryptophan 6-halogenase (SttH), which enabled key residues that contribute to the regioselectivity in tryptophan halogenases to be identified. Structure-guided mutagenesis resulted in a triple mutant (L460F/P461E/P462T) that exhibited a complete switch in regioselectivity; with the substrate 3-indolepropionate 75% 5-chlorination was observed with the mutant in comparison with 90% 6-chlorination for the wild-type SttH. This is the first clear example of how regiocomplementary halogenase enzymes can be created from a single parent enzyme. The biocatalytic repertoire of SttH was also expanded to include a range of indolic and non-indolic substrates.
-
-	A Structure-Guided Switch in the Regioselectivity of a Tryptophan Halogenase Enzyme.,Shepherd SA, Menon BR, Fisk H, Struck AW, Levy C, Leys D, Micklefield J Chembiochem. 2016 Feb 3. doi: 10.1002/cbic.201600051. PMID:26840773<ref>PMID:26840773</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5hy5" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:00, 16 August 2023

OCA — Wed, 16 Aug 2023 11:00:03 GMT

←Older revision		Revision as of 11:00, 16 August 2023
Line 1:		Line 1:

	==Crystal Structure of a Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini==		==Crystal Structure of a Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini==
-	<StructureSection load='5hy5' size='340' side='right' caption='[[5hy5]], [[Resolution\|resolution]] 2.68Å' scene=''>	+	<StructureSection load='5hy5' size='340' side='right'caption='[[5hy5]], [[Resolution\|resolution]] 2.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hy5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"actinomyces_toxytricini"_preobrazhenskaya_and_sveshnikova_in_gauze_et_al._1957 "actinomyces~~ toxytricini~~" preobrazhenskaya and sveshnikova in gauze et al. 1957~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HY5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5HY5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hy5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_toxytricini Streptomyces toxytricini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HY5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HY5 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CL:CHLORIDE+ION'>CL</scene~~>, ~~<scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.68Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~stth ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=67369 "Actinomyces toxytricini" Preobrazhenskaya and Sveshnikova in Gauze et al. 1957])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5hy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hy5 OCA], [~~http~~://pdbe.org/5hy5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5hy5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5hy5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5hy5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hy5 OCA], [https://pdbe.org/5hy5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hy5 RCSB], [https://www.ebi.ac.uk/pdbsum/5hy5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hy5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/E9P162_9ACTN E9P162_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Actinomyces toxytricini preobrazhenskaya and sveshnikova in gauze et al. 1957~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Levy, C~~]]	+	[[Category: Streptomyces toxytricini]]
-	[[Category: ~~Oxidoreductase]]~~	+	[[Category: Levy C]]
-	~~[[Category: Tryptophan halogenase~~]]	+

OCA at 13:32, 16 November 2017

OCA — Thu, 16 Nov 2017 13:32:53 GMT

←Older revision		Revision as of 13:32, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='5hy5' size='340' side='right' caption='[[5hy5]], [[Resolution\|resolution]] 2.68Å' scene=''>		<StructureSection load='5hy5' size='340' side='right' caption='[[5hy5]], [[Resolution\|resolution]] 2.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5hy5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HY5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5hy5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_toxytricini"_preobrazhenskaya_and_sveshnikova_in_gauze_et_al._1957 "actinomyces toxytricini" preobrazhenskaya and sveshnikova in gauze et al. 1957]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HY5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hy5 OCA], [http://pdbe.org/5hy5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hy5 RCSB], [http://www.ebi.ac.uk/pdbsum/5hy5 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">stth ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=67369 "Actinomyces toxytricini" Preobrazhenskaya and Sveshnikova in Gauze et al. 1957])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hy5 OCA], [http://pdbe.org/5hy5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hy5 RCSB], [http://www.ebi.ac.uk/pdbsum/5hy5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hy5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actinomyces toxytricini preobrazhenskaya and sveshnikova in gauze et al. 1957]]
	[[Category: Levy, C]]		[[Category: Levy, C]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Tryptophan halogenase]]		[[Category: Tryptophan halogenase]]

OCA at 02:42, 21 February 2016

OCA — Sun, 21 Feb 2016 02:42:22 GMT

←Older revision		Revision as of 02:42, 21 February 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5hy5 is ~~ON HOLD~~	+	==Crystal Structure of a Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini==
		+	<StructureSection load='5hy5' size='340' side='right' caption='[[5hy5]], [[Resolution\|resolution]] 2.68Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5hy5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HY5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hy5 OCA], [http://pdbe.org/5hy5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hy5 RCSB], [http://www.ebi.ac.uk/pdbsum/5hy5 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flavin-dependent halogenase enzymes are potentially useful biocatalysts for the regioselectively halogenation of aromatic compounds. Haloaromatic compounds can be utilised in the synthesis and biosynthesis of pharmaceuticals and other valuable products. Here we report the first X-ray crystal structure of a tryptophan 6-halogenase (SttH), which enabled key residues that contribute to the regioselectivity in tryptophan halogenases to be identified. Structure-guided mutagenesis resulted in a triple mutant (L460F/P461E/P462T) that exhibited a complete switch in regioselectivity; with the substrate 3-indolepropionate 75% 5-chlorination was observed with the mutant in comparison with 90% 6-chlorination for the wild-type SttH. This is the first clear example of how regiocomplementary halogenase enzymes can be created from a single parent enzyme. The biocatalytic repertoire of SttH was also expanded to include a range of indolic and non-indolic substrates.

-	~~Authors: Levy~~, C.	+	A Structure-Guided Switch in the Regioselectivity of a Tryptophan Halogenase Enzyme.,Shepherd SA, Menon BR, Fisk H, Struck AW, Levy C, Leys D, Micklefield J Chembiochem. 2016 Feb 3. doi: 10.1002/cbic.201600051. PMID:26840773<ref>PMID:26840773</ref>

-	~~Description: Crystal Structure of~~ a ~~Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5hy5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Levy, C]]		[[Category: Levy, C]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Tryptophan halogenase]]

OCA: Protected "5hy5" [edit=sysop:move=sysop]

OCA — Wed, 10 Feb 2016 15:53:07 GMT

Protected "5hy5" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:53, 10 February 2016

OCA: New page: '''Unreleased structure''' The entry 5hy5 is ON HOLD Authors: Levy, C. Description: Crystal Structure of a Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini [[Category: Unre...

OCA — Wed, 10 Feb 2016 15:53:05 GMT

New page: '''Unreleased structure''' The entry 5hy5 is ON HOLD Authors: Levy, C. Description: Crystal Structure of a Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini [[Category: Unre...

New page

'''Unreleased structure'''

The entry 5hy5 is ON HOLD

Authors: Levy, C.

Description: Crystal Structure of a Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini
[[Category: Unreleased Structures]]
[[Category: Levy, C]]